Skip to main content
NCBI home page
Infection and Immunity logoLink to Infection and Immunity
. 1979 Oct;26(1):225–231. doi: 10.1128/iai.26.1.225-231.1979

Surface markers and size of lymphocytes in human umbilical cord blood stimulated into deoxyribonucleic acid synthesis by Epstein-Barr Virus.

J Robinson, A Frank, E Henderson, J Schweitzer, G Miller
PMCID: PMC414599  PMID: 227794

Abstract

We characterized subpopulations of lymphocytes in human umbilical cord blood which are stimulated into deoxyribonucleic acid synthesis by Epstein-Barr virus. Lymphocytes were examined simultaneously for deoxyribonucleic acid synthesis by autoradiography and for surface markers by rosette formation with sheep erythrocytes or erythrocytes coated with antibody and mouse complement (EAC). The subpopulation which incorporated [3H]thymidine after exposure to virus consisted mainly of cells which formed rosettes with EAC. Lymphocytes were enriched or depleted of thymus-derived lymphocytes (T cells), null cells, or cells forming rosettes with EAC. The extent of sensitivity of the cells to stimulation by Epstein-Barr virus correlated with the proportion of the population which formed rosettes with EAC. When mononuclear cell populations were depleted of T lymphocytes and then fractionated by size, small lymphocytes showed higher rates of deoxyribonucleic acid synthesis after virus exposure and higher transformation frequency than did larger cells or unfractionated cells. Thus, the cells which are stimulated into deoxyribonucleic acid synthesis by Epstein-Barr virus appear to be the same as cells which are ultimately transformed.

Full text

PDF
225

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ehlenberger A. G., McWilliams M., Phillips-Quagliata J. M., Lamm M. E., Nussenzweig V. Immunoglobulin-bearing and complement-receptor lymphocytes constitute the same population in human peripheral blood. J Clin Invest. 1976 Jan;57(1):53–56. doi: 10.1172/JCI108268. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Einhorn L., Steinitz M., Yefenof E., Ernberg I., Bakacs T., Klein G. Epstein-Barr virus (EBV) receptors, complement receptors, and EBV infectibility of different lymphocyte fractions of human peripheral blood. II. Epstein-Barr virus studies. Cell Immunol. 1978 Jan;35(1):43–58. doi: 10.1016/0008-8749(78)90125-9. [DOI] [PubMed] [Google Scholar]
  3. Gerber P., Hoyer B. H. Induction of cellular DNA synthesis in human leukocytes by Epstein-Barr virus. Nature. 1971 May 7;231(5297):46–47. doi: 10.1038/231046a0. [DOI] [PubMed] [Google Scholar]
  4. Henderson E., Miller G., Robinson J., Heston L. Efficiency of transformation of lymphocytes by Epstein-Barr virus. Virology. 1977 Jan;76(1):152–163. doi: 10.1016/0042-6822(77)90292-6. [DOI] [PubMed] [Google Scholar]
  5. Henderson E., Robinson J., Frank A., Miller G. Epstein-Barr virus: transformation of lymphocytes separated by size or exposed to bromodeoxyuridine and light. Virology. 1977 Oct 1;82(1):196–205. doi: 10.1016/0042-6822(77)90042-3. [DOI] [PubMed] [Google Scholar]
  6. Jondal M., Klein G. Surface markers on human B and T lymphocytes. II. Presence of Epstein-Barr virus receptors on B lymphocytes. J Exp Med. 1973 Dec 1;138(6):1365–1378. doi: 10.1084/jem.138.6.1365. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Loos J. A., Roos D. Ficoll-isopaque gradients for the determination of density distributions of human blood lymphocytes and other reticulo-endothelial cells. Exp Cell Res. 1974 Jun;86(2):333–341. doi: 10.1016/0014-4827(74)90721-6. [DOI] [PubMed] [Google Scholar]
  8. Miller G., Lipman M. Comparison of the yield of infectious virus from clones of human and simian lymphoblastoid lines transformed by Epstein-Barr virus. J Exp Med. 1973 Dec 1;138(6):1398–1412. doi: 10.1084/jem.138.6.1398. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Mogensen C. E. The glomerular permeability determined by dextran clearance using Sephadex gel filtration. Scand J Clin Lab Invest. 1968;21(1):77–82. doi: 10.3109/00365516809076979. [DOI] [PubMed] [Google Scholar]
  10. Pattengale P. K., Smith R. W., Gerber P. B-cell characteristics of human peripheral and cord blood lymphocytes transformed by Epstein-Barr virus. J Natl Cancer Inst. 1974 Apr;52(4):1081–1086. doi: 10.1093/jnci/52.4.1081. [DOI] [PubMed] [Google Scholar]
  11. Robinson J. E., Andiman W. A., Henderson E., Miller G. Host-determined differences in expression of surface marker characteristics on human and simian lymphoblastoid cell lines transformed by Epstein-Barr virus. Proc Natl Acad Sci U S A. 1977 Feb;74(2):749–753. doi: 10.1073/pnas.74.2.749. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Robinson J. Assay for Epstein-Barr virus based on stimulation of DNA synthesis in mixed leukocytes from human umbilical cord blood. J Virol. 1975 May;15(5):1065–1072. doi: 10.1128/jvi.15.5.1065-1072.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Schneider U., zur Hausen H. Epstein-Barr virus-induced transformation of human leukocytes after cell fractionation. Int J Cancer. 1975 Jan 15;15(1):59–66. doi: 10.1002/ijc.2910150108. [DOI] [PubMed] [Google Scholar]
  14. Splinter T. A., Reiss M. Separation of lymphoid-line cells according to volume and density. Exp Cell Res. 1974 Dec;89(2):343–351. doi: 10.1016/0014-4827(74)90799-x. [DOI] [PubMed] [Google Scholar]
  15. Stoker M. Abortive transformation by polyoma virus. Nature. 1968 Apr 20;218(5138):234–238. doi: 10.1038/218234a0. [DOI] [PubMed] [Google Scholar]
  16. Weiner M. S., Bianco C., Nussenzweig V. Enhanced binding of neuraminidase-treated sheep erythrocytes to human T lymphocytes. Blood. 1973 Dec;42(6):939–946. [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES