Skip to main content
PMC home page
Infection and Immunity logoLink to Infection and Immunity
. 1979 Oct;26(1):298–304. doi: 10.1128/iai.26.1.298-304.1979

Fatty acid composition of gliding bacteria: oral isolates of Capnocytophaga compared with Sporocytophaga.

S C Holt, G Forcier, B J Takacs
PMCID: PMC414611  PMID: 500207

Abstract

The extractable and bound lipids and cellular fatty acids of the gram-negative gliding bacteria, Capnocytophaga sputigena, C. gingivalis, and C. ochracea were compared to the non-host-related gliding bacterium Sporocytophaga myxococcoides. The extractable lipids represented between 17 and 28% of the cell dry weight, whereas only 2 to 4% of the lipids were in the bound fraction. The methyl esters of the cellular fatty acids were mainly aC15:0, which accounted for 69 to 73% of the total extractable fatty acids; S. myxococcoides had a similar distribution of branched-chain fatty acids; however, aC17:0 was the predominant fatty acid in this free-living gliding organism.

Full text

PDF
298

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ABEL K., DESCHMERTZING H., PETERSON J. I. CLASSIFICATION OF MICROORGANISMS BY ANALYSIS OF CHEMICAL COMPOSITION. I. FEASIBILITY OF UTILIZING GAS CHROMATOGRAPHY. J Bacteriol. 1963 May;85:1039–1044. doi: 10.1128/jb.85.5.1039-1044.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Amdur B. H., Szabo E. I., Socransky S. S. Fatty acids of Gram-positive bacterial rods from human dental plaque. Arch Oral Biol. 1978;23(1):23–29. doi: 10.1016/0003-9969(78)90049-3. [DOI] [PubMed] [Google Scholar]
  3. Breznak J. A., Canale-Parola E. Metabolism of Spirochaeta aurantia. II. Aerobic oxidation oxidation of carbohydrates. Arch Mikrobiol. 1972;83(4):278–292. doi: 10.1007/BF00425240. [DOI] [PubMed] [Google Scholar]
  4. CARROLL K. K. Quantitative estimation of peak areas in gas-liquid chromatography. Nature. 1961 Jul 22;191:377–378. doi: 10.1038/191377a0. [DOI] [PubMed] [Google Scholar]
  5. CHARLES A. B., BARRETT F. C. DETECTION OF VOLATILE FATTY ACIDS PRODUCED BY OBLIGATE GRAM-NEGATIVE ANAEROBES. J Med Lab Technol. 1963 Oct;20:266–268. [PubMed] [Google Scholar]
  6. Carlsson J. Simplified gas chromatographic procedure for identification of bacterial metabolic products. Appl Microbiol. 1973 Feb;25(2):287–289. doi: 10.1128/am.25.2.287-289.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. HUSTON C. K., ALBRO P. W. LIPIDS OF SARCINA LUTEA. I. FATTY ACID COMPOSITION OF THE EXTRACTABLE LIPIDS. J Bacteriol. 1964 Aug;88:425–432. doi: 10.1128/jb.88.2.425-432.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Holt S. C., Leadbetter E. R. Fine structure of Sporocytophaga myxococcoides. Arch Mikrobiol. 1967 Jun 21;57(3):199–213. doi: 10.1007/BF00405947. [DOI] [PubMed] [Google Scholar]
  9. Holt S. C., Leadbetter E. R., Socransky S. S. Capnocytophaga: new genus of gram-negative gliding bacteria. II. Morphology and ultrastructure. Arch Microbiol. 1979 Jul;122(1):17–27. doi: 10.1007/BF00408041. [DOI] [PubMed] [Google Scholar]
  10. Irving J. T., Socransky S. S., Tanner A. C. Histological changes in experimental periodontal disease in rats monoinfected with gram-negative organisms. J Periodontal Res. 1978 Jul;13(4):326–332. doi: 10.1111/j.1600-0765.1978.tb00187.x. [DOI] [PubMed] [Google Scholar]
  11. JAMES A. T., WHEATLEY V. R. Studies of sebum. 6. The determination of the component fatty acids of human forearm sebum by gas-liquid chromatography. Biochem J. 1956 Jun;63(2):269–273. doi: 10.1042/bj0630269. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Joseph R. Fatty acid composition of Spirochaeta stenostrepta. J Bacteriol. 1972 Oct;112(1):629–631. doi: 10.1128/jb.112.1.629-631.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. KANESHIRO T., MARR A. G. Hydroxy fatty acids of Azotobacter agilis. Biochim Biophys Acta. 1963 Jun 18;70:271–277. doi: 10.1016/0006-3002(63)90751-0. [DOI] [PubMed] [Google Scholar]
  14. Kaneda T. Factors affecting the relative ratio of fatty acids in Bacillus cereus. Can J Microbiol. 1971 Feb;17(2):269–275. doi: 10.1139/m71-045. [DOI] [PubMed] [Google Scholar]
  15. Kaneda T. Fatty acids in the genus Bacillus. I. Iso- and anteiso-fatty acids as characteristic constituents of lipids in 10 species. J Bacteriol. 1967 Mar;93(3):894–903. doi: 10.1128/jb.93.3.894-903.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Kaneda T. Fatty acids of the genus Bacillus: an example of branched-chain preference. Bacteriol Rev. 1977 Jun;41(2):391–418. doi: 10.1128/br.41.2.391-418.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Kramzar G. R., Lynch D. L. A qualitative and quantitative study of the fatty acid composition of selected micro-organisms. Microbios. 1976;17(67):7–16. [PubMed] [Google Scholar]
  18. Kunsman J. E. Characterization of the lipids of six strains of Bacteroides ruminicola. J Bacteriol. 1973 Mar;113(3):1121–1126. doi: 10.1128/jb.113.3.1121-1126.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Leadbetter E. R., Holt S. C., Socransky S. S. Capnocytophaga: new genus of gram-negative gliding bacteria. I. General characteristics, taxonomic considerations and significance. Arch Microbiol. 1979 Jul;122(1):9–16. doi: 10.1007/BF00408040. [DOI] [PubMed] [Google Scholar]
  20. Lindner J. G., Marcelis J. H. Quantitative gas chromatography of Bacteroides species under different growth conditions. Antonie Van Leeuwenhoek. 1978;44(1):1–14. doi: 10.1007/BF00400072. [DOI] [PubMed] [Google Scholar]
  21. Livermore B. P., Johnson R. C. Lipids of the Spirochaetales: comparison of the lipids of several members of the genera Spirochaeta, Treponema, and Leptospira. J Bacteriol. 1974 Dec;120(3):1268–1273. doi: 10.1128/jb.120.3.1268-1273.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Marr A. G., Ingraham J. L. EFFECT OF TEMPERATURE ON THE COMPOSITION OF FATTY ACIDS IN ESCHERICHIA COLI. J Bacteriol. 1962 Dec;84(6):1260–1267. doi: 10.1128/jb.84.6.1260-1267.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Moss C. W., Cherry W. B. Characterization of the C15 branched-chain fatty acids of Corynebacterium acnes by gas chromatography. J Bacteriol. 1968 Jan;95(1):241–242. doi: 10.1128/jb.95.1.241-242.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Moss C. W., Dees S. B. Identification of microorganisms by gas chromatographic-mass spectrometric analysis of cellular fatty acids. J Chromatogr. 1975 Oct 29;112:594–604. doi: 10.1016/s0021-9673(00)99988-6. [DOI] [PubMed] [Google Scholar]
  25. Moss C. W., Dowell V. R., Jr, Farshtchi D., Raines L. J., Cherry W. B. Cultural characteristics and fatty acid composition of propionibacteria. J Bacteriol. 1969 Feb;97(2):561–570. doi: 10.1128/jb.97.2.561-570.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Moss C. W., Dowell V. R., Jr, Lewis V. J., Schekter M. A. Cultural characteristics and fatty acid composition of Corynebacterium acnes. J Bacteriol. 1967 Nov;94(5):1300–1305. doi: 10.1128/jb.94.5.1300-1305.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Moss C. W., Weaver R. E., Dees S. B., Cherry W. B. Cellular fatty acid composition of isolates from Legionnaires disease. J Clin Microbiol. 1977 Aug;6(2):140–143. doi: 10.1128/jcm.6.2.140-143.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Newman M. G., Socransky S. S. Predominant cultivable microbiota in periodontosis. J Periodontal Res. 1977 Mar;12(2):120–128. doi: 10.1111/j.1600-0765.1977.tb00114.x. [DOI] [PubMed] [Google Scholar]
  29. Newman M. G., Socransky S. S., Savitt E. D., Propas D. A., Crawford A. Studies of the microbiology of periodontosis. J Periodontol. 1976 Jul;47(7):373–379. doi: 10.1902/jop.1976.47.7.373. [DOI] [PubMed] [Google Scholar]
  30. Oshima M., Miyagawa A. Comparative studies on the fatty acid composition of moderately and extremely thermophilic bacteria. Lipids. 1974 Jul;9(7):476–480. doi: 10.1007/BF02534274. [DOI] [PubMed] [Google Scholar]
  31. Pandhi P. N., Hammond B. F. A glycolipid from Rothia dentocariosa. Arch Oral Biol. 1975 May-Jun;20(5-6):399–401. doi: 10.1016/0003-9969(75)90035-7. [DOI] [PubMed] [Google Scholar]
  32. Raines L. J., Moss C. W., Farshtchi D., Pittman B. Fatty acids of Listeria monocytogenes. J Bacteriol. 1968 Dec;96(6):2175–2177. doi: 10.1128/jb.96.6.2175-2177.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  33. Rizza V., Tucker A. N., White D. C. Lipids of Bacteroides melaninogenicus. J Bacteriol. 1970 Jan;101(1):84–91. doi: 10.1128/jb.101.1.84-91.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  34. Rosenfelder G., Lüderitz O., Westphal O. Composition of lipopolysaccharides from Myxococcus fulvus and other fruiting and non-fruiting myxobacteria. Eur J Biochem. 1974 May 15;44(2):411–420. doi: 10.1111/j.1432-1033.1974.tb03499.x. [DOI] [PubMed] [Google Scholar]
  35. Slots J. The predominant cultivable organisms in juvenile periodontitis. Scand J Dent Res. 1976 Jan;84(1):1–10. doi: 10.1111/j.1600-0722.1976.tb00454.x. [DOI] [PubMed] [Google Scholar]
  36. Stanier R. Y. THE CYTOPHAGA GROUP: A CONTRIBUTION TO THE BIOLOGY OF MYXOBACTERIA. Bacteriol Rev. 1942 Sep;6(3):143–196. doi: 10.1128/br.6.3.143-196.1942. [DOI] [PMC free article] [PubMed] [Google Scholar]
  37. Tornabene T. G., Bennett E. O., Oró J. Fatty acid and aliphatic hydrocarbon composition of Sarcina lutea grown in three different media. J Bacteriol. 1967 Aug;94(2):344–348. doi: 10.1002/path.1700940212. [DOI] [PMC free article] [PubMed] [Google Scholar]
  38. WELLS M. A., DITTMER J. C. THE USE OF SEPHADEX FOR THE REMOVAL OF NONLIPID CONTAMINANTS FROM LIPID EXTRACTS. Biochemistry. 1963 Nov-Dec;2:1259–1263. doi: 10.1021/bi00906a015. [DOI] [PubMed] [Google Scholar]
  39. Walker R. W. Cis-11-hexadecenoic acid from Cytophaga hutchinsonii lipids. Lipids. 1969 Jan;4(1):15–18. doi: 10.1007/BF02531788. [DOI] [PubMed] [Google Scholar]
  40. Ware J. C., Dworkin M. Fatty acids of Myxococcus xanthus. J Bacteriol. 1973 Jul;115(1):253–261. doi: 10.1128/jb.115.1.253-261.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  41. Weinbaum G., Panos C. Fatty acid distribution in normal and filamentous Escherichia coli. J Bacteriol. 1966 Nov;92(5):1576–1577. doi: 10.1128/jb.92.5.1576-1577.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  42. Williams B. L., Pantalone R. M., Sherris J. C. Subgingival microflora and periodontitis. J Periodontal Res. 1976 Feb;11(1):1–18. doi: 10.1111/j.1600-0765.1976.tb00045.x. [DOI] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES