Skip to main content
Infection and Immunity logoLink to Infection and Immunity
. 1979 Oct;26(1):305–310. doi: 10.1128/iai.26.1.305-310.1979

Phospholipid composition of gliding bacteria: oral isolates of Capnocytophaga compared with Sporocytophaga.

S C Holt, J Doundowlakis, B J Takacs
PMCID: PMC414612  PMID: 500209

Abstract

The distribution of acetone-soluble (neutral glycolipid) and acetone-insoluble (phospholipid isoprenoids) lipids in oral isolates of gram-negative gliding bacteria of the genus Capnocytophaga was compared with those in a non-host-related gliding bacterium, Sporocytophaga myxococcoides. The acetone-soluble material accounted for 34 to 55% of the extracted lipids; the remainder was acetone-insoluble material. The major phospholipid was phosphatidylethanolamine (67%), with lesser amounts of lysophosphatidylethanolamine and several unidentified phosphate-containing compounds. Capnocytophaga also contained significant amounts of an ornithine-amino lipid.

Full text

PDF
305

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. AMES B. N., DUBIN D. T. The role of polyamines in the neutralization of bacteriophage deoxyribonucleic acid. J Biol Chem. 1960 Mar;235:769–775. [PubMed] [Google Scholar]
  2. Ames G. F. Lipids of Salmonella typhimurium and Escherichia coli: structure and metabolism. J Bacteriol. 1968 Mar;95(3):833–843. doi: 10.1128/jb.95.3.833-843.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. BLIGH E. G., DYER W. J. A rapid method of total lipid extraction and purification. Can J Biochem Physiol. 1959 Aug;37(8):911–917. doi: 10.1139/o59-099. [DOI] [PubMed] [Google Scholar]
  4. Canale-Parola E. Physiology and evolution of spirochetes. Bacteriol Rev. 1977 Mar;41(1):181–204. doi: 10.1128/br.41.1.181-204.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Cronan J. E., Jr Phospholipid alterations during growth of Escherichia coli. J Bacteriol. 1968 Jun;95(6):2054–2061. doi: 10.1128/jb.95.6.2054-2061.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. DITTMER J. C., LESTER R. L. A SIMPLE, SPECIFIC SPRAY FOR THE DETECTION OF PHOSPHOLIPIDS ON THIN-LAYER CHROMATOGRAMS. J Lipid Res. 1964 Jan;5:126–127. [PubMed] [Google Scholar]
  7. De Siervo A. J. Alterations in the phospholipid composition of Escherichia coli B during growth at different temperatures. J Bacteriol. 1969 Dec;100(3):1342–1349. doi: 10.1128/jb.100.3.1342-1349.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. FOLCH J., LEES M., SLOANE STANLEY G. H. A simple method for the isolation and purification of total lipides from animal tissues. J Biol Chem. 1957 May;226(1):497–509. [PubMed] [Google Scholar]
  9. Heefner D. L., Claus G. W. Lipid and fatty acid composition of Gluconobacter oxydans before and after intracytoplasmic membrane formation. J Bacteriol. 1978 Apr;134(1):38–47. doi: 10.1128/jb.134.1.38-47.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Holt S. C. Anatomy and chemistry of spirochetes. Microbiol Rev. 1978 Mar;42(1):114–160. doi: 10.1128/mr.42.1.114-160.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Holt S. C., Forcier G., Takacs B. J. Fatty acid composition of gliding bacteria: oral isolates of Capnocytophaga compared with Sporocytophaga. Infect Immun. 1979 Oct;26(1):298–304. doi: 10.1128/iai.26.1.298-304.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Holt S. C., Leadbetter E. R. Fine structure of Sporocytophaga myxococcoides. Arch Mikrobiol. 1967 Jun 21;57(3):199–213. doi: 10.1007/BF00405947. [DOI] [PubMed] [Google Scholar]
  13. Holt S. C., Leadbetter E. R., Socransky S. S. Capnocytophaga: new genus of gram-negative gliding bacteria. II. Morphology and ultrastructure. Arch Microbiol. 1979 Jul;122(1):17–27. doi: 10.1007/BF00408041. [DOI] [PubMed] [Google Scholar]
  14. Ikawa M. Bacterial phosphatides and natural relationships. Bacteriol Rev. 1967 Mar;31(1):54–64. doi: 10.1128/br.31.1.54-64.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Irving J. T., Socransky S. S., Tanner A. C. Histological changes in experimental periodontal disease in rats monoinfected with gram-negative organisms. J Periodontal Res. 1978 Jul;13(4):326–332. doi: 10.1111/j.1600-0765.1978.tb00187.x. [DOI] [PubMed] [Google Scholar]
  16. Johnson R. C., Livermore B. P., Walby J. K., Jenkin H. M. Lipids of parasitic and saprophytic leptospires. Infect Immun. 1970 Sep;2(3):286–291. doi: 10.1128/iai.2.3.286-291.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Johnson R. C. The spirochetes. Annu Rev Microbiol. 1977;31:89–106. doi: 10.1146/annurev.mi.31.100177.000513. [DOI] [PubMed] [Google Scholar]
  18. Joseph R., Holt S. C., Canale-Parola E. Peptidoglycan of free-living anaerobic spirochetes. J Bacteriol. 1973 Jul;115(1):426–435. doi: 10.1128/jb.115.1.426-435.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. KENNEDY E. P. Biosynthesis of complex lipids. Fed Proc. 1961 Dec;20:934–940. [PubMed] [Google Scholar]
  20. Kanemasa Y., Akamatsu Y., Nojima S. Composition and turnover of the phospholipids in Escherichia coli. Biochim Biophys Acta. 1967 Oct 2;144(2):382–390. [PubMed] [Google Scholar]
  21. Leadbetter E. R., Holt S. C., Socransky S. S. Capnocytophaga: new genus of gram-negative gliding bacteria. I. General characteristics, taxonomic considerations and significance. Arch Microbiol. 1979 Jul;122(1):9–16. doi: 10.1007/BF00408040. [DOI] [PubMed] [Google Scholar]
  22. Livermore B. P., Johnson R. C. Isolation and characterization of a glycolipid from Treponema pallidum, Kazan 5. Biochim Biophys Acta. 1970 Jul 14;210(2):315–318. doi: 10.1016/0005-2760(70)90176-1. [DOI] [PubMed] [Google Scholar]
  23. Livermore B. P., Johnson R. C. Lipids of the Spirochaetales: comparison of the lipids of several members of the genera Spirochaeta, Treponema, and Leptospira. J Bacteriol. 1974 Dec;120(3):1268–1273. doi: 10.1128/jb.120.3.1268-1273.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. MUNIER R. Microchromatographie de partage sur papier des alcaloïdes et de diverses bases azotées biologiques. V. Séparation'des constituants azotés des phosphoaminolipides (choline, colamine, sérine). Bull Soc Chim Biol (Paris) 1951;33(7):862–867. [PubMed] [Google Scholar]
  25. Makula R. A., Finnerty W. R. Isolation and characterization of an ornithine-containing lipid from Desulfovibrio gigas. J Bacteriol. 1975 Aug;123(2):523–529. doi: 10.1128/jb.123.2.523-529.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Makula R. A., Finnerty W. R. Phospholipid composition of Desulfovibrio species. J Bacteriol. 1974 Dec;120(3):1279–1283. doi: 10.1128/jb.120.3.1279-1283.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Meyer H., Meyer F. Lipid metabolism in the parasitic and free-living spirochetes Treponema pallidum (Reiter) and Treponema zuelzerae. Biochim Biophys Acta. 1971 Feb 2;231(1):93–106. doi: 10.1016/0005-2760(71)90257-8. [DOI] [PubMed] [Google Scholar]
  28. Newman M. G., Socransky S. S. Predominant cultivable microbiota in periodontosis. J Periodontal Res. 1977 Mar;12(2):120–128. doi: 10.1111/j.1600-0765.1977.tb00114.x. [DOI] [PubMed] [Google Scholar]
  29. Newman M. G., Socransky S. S., Savitt E. D., Propas D. A., Crawford A. Studies of the microbiology of periodontosis. J Periodontol. 1976 Jul;47(7):373–379. doi: 10.1902/jop.1976.47.7.373. [DOI] [PubMed] [Google Scholar]
  30. Oliver J. D., Colwell R. R. Extractable lipids of gram-negative marine bacteria: phospholipid composition. J Bacteriol. 1973 Jun;114(3):897–908. doi: 10.1128/jb.114.3.897-908.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Olsen R. W., Ballou C. E. Acyl phosphatidylglycerol. A new phospholipid from Salmonella typhimurium. J Biol Chem. 1971 May 25;246(10):3305–3313. [PubMed] [Google Scholar]
  32. PIERINGER R. A., KUNNES R. S. THE BIOSYNTHESIS OF PHOSPHATIDIC ACID AND LYSOPHOSPHATIDIC ACID BY GLYCERIDE PHOSPHOKINASE PATHWAYS IN ESCHERICHIA COLI. J Biol Chem. 1965 Jul;240:2833–2838. [PubMed] [Google Scholar]
  33. Randle C. L., Albro P. W., Dittmer J. C. The phosphoglyceride composition of Gram-negative bacteria and the changes in composition during growth. Biochim Biophys Acta. 1969;187(2):214–220. doi: 10.1016/0005-2760(69)90030-7. [DOI] [PubMed] [Google Scholar]
  34. Schleifer K. H., Joseph R. A directly cross-linked L-ornithine-containing peptidoglycan in cell walls of Spirochaeta stenostrepta. FEBS Lett. 1973 Oct 1;36(1):83–86. doi: 10.1016/0014-5793(73)80342-4. [DOI] [PubMed] [Google Scholar]
  35. Shively J. M., Knoche H. W. Isolation of an ornithine-containing lipid from Thiobacillus thiooxidans. J Bacteriol. 1969 May;98(2):829–830. doi: 10.1128/jb.98.2.829-830.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  36. Slots J. The predominant cultivable organisms in juvenile periodontitis. Scand J Dent Res. 1976 Jan;84(1):1–10. doi: 10.1111/j.1600-0722.1976.tb00454.x. [DOI] [PubMed] [Google Scholar]
  37. Socransky S. S., Holt S. C., Leadbetter E. R., Tanner A. C., Savitt E., Hammond B. F. Capnocytophaga: new genus of gram-negative gliding bacteria. III. Physiological characterization. Arch Microbiol. 1979 Jul;122(1):29–33. doi: 10.1007/BF00408042. [DOI] [PubMed] [Google Scholar]
  38. Sud I. J., Feingold D. S. Mechanism of polymyxin B resistance in Proteus mirabilis. J Bacteriol. 1970 Oct;104(1):289–294. doi: 10.1128/jb.104.1.289-294.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  39. Sud I. J., Feingold D. S. Phospholipids and fatty acids of Neisseria gonorrhoeae. J Bacteriol. 1975 Nov;124(2):713–717. doi: 10.1128/jb.124.2.713-717.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  40. Szabo E. I., Amdur B. H., Socransky S. S. Lipid composition of Streptococcus mutans. Caries Res. 1978;12(1):21–27. doi: 10.1159/000260311. [DOI] [PubMed] [Google Scholar]
  41. Thiele O. W., Schwinn G. Bakterielle Ornithinlipide. Z Allg Mikrobiol. 1974;14(5):435–443. doi: 10.1002/jobm.3630140509. [DOI] [PubMed] [Google Scholar]
  42. Thiele O. W., Schwinn G. The free lipids of Brucella melitensis and Bordetella pertussis. Eur J Biochem. 1973 Apr;34(2):333–344. doi: 10.1111/j.1432-1033.1973.tb02764.x. [DOI] [PubMed] [Google Scholar]
  43. Vaskovsky V. E., Kostetsky E. Y. Modified spray for the detection of phospholipids on thin-layer chromatograms. J Lipid Res. 1968 May;9(3):396–396. [PubMed] [Google Scholar]
  44. White D. C., Frerman F. E. Extraction, characterization, and cellular localization of the lipids of Staphylococcus aureus. J Bacteriol. 1967 Dec;94(6):1854–1867. doi: 10.1128/jb.94.6.1854-1867.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  45. Wilkinson S. G. Composition and structure of the ornithine-containing lipid from Pseudomonas rubescens. Biochim Biophys Acta. 1972 May 23;270(1):1–17. doi: 10.1016/0005-2760(72)90171-3. [DOI] [PubMed] [Google Scholar]
  46. Winkler H. H., Miller E. T. Phospholipid composition of Rickettsia prowazeki grown in chicken embryo yolk sacs. J Bacteriol. 1978 Oct;136(1):175–178. doi: 10.1128/jb.136.1.175-178.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  47. van Palenstein Helderman W. H. Total viable count and differential count of vibrio (campylobacter) sputorum, fusobacterium nucleatum, selenomonas sputigena, bacteroides ochraceus and veillonella in the inflamed and non inflamed human gingival crevice. J Periodontal Res. 1975 Nov;10(5):294–305. doi: 10.1111/j.1600-0765.1975.tb00037.x. [DOI] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES