Abstract
The behavior of a surface antigenic determinant (SA) of lateral flagella of Vibrio parahaemolyticus was studied. SA, located on the surface of flagellar filaments, is responsible for H-agglutination and specific for serotype. Results of H-agglutination inhibition tests demonstrated that SA could not be detected on the flagellin molecule when the flagellar filaments were dissociated to flagellin monomers by heating or treatment with urea, sodium dodecyl sulfate, HCl, or acetone, although SA could be detected on short flagellar fragments obtained by milder heat treatment. When flagellar filaments were reconstituted from flagellin monomers, SA was detected on the surface of the filaments. These results suggest that SA is buried in the flagellin molecule with dissociation of flagellar filaments to flagellin monomers or steric configuration of SA itself is altered to a different form which cannot react with the responsible antibody.
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- ABRAM D., KOFFLER H. IN VITRO FORMATION OF FLAGELLA-LIKE FILAMENTS AND OTHER STRUCTURES FROM FLAGELLIN. J Mol Biol. 1964 Jul;9:168–185. doi: 10.1016/s0022-2836(64)80098-x. [DOI] [PubMed] [Google Scholar]
- ASAKURA S., EGUCHI G., IINO T. RECONSTITUTION OF BACTERIAL FLAGELLA IN VITRO. J Mol Biol. 1964 Oct;10:42–56. doi: 10.1016/s0022-2836(64)80026-7. [DOI] [PubMed] [Google Scholar]
- Allen R. D., Baumann P. Structure and arrangement of flagella in species of the genus Beneckea and Photobacterium fischeri. J Bacteriol. 1971 Jul;107(1):295–302. doi: 10.1128/jb.107.1.295-302.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Baumann P., Baumann L., Mandel M. Taxonomy of marine bacteria: the genus Beneckea. J Bacteriol. 1971 Jul;107(1):268–294. doi: 10.1128/jb.107.1.268-294.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Baumann P., Baumann L., Reichelt J. L. Taxonomy of marine bacteria: Beneckea parahaemolytica and Beneckea alginolytica. J Bacteriol. 1973 Mar;113(3):1144–1155. doi: 10.1128/jb.113.3.1144-1155.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
- LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
- Sheffery M., Newton A. Reconstitution and purification of flagellar filaments from Caulobacter crescentus. J Bacteriol. 1977 Dec;132(3):1027–1030. doi: 10.1128/jb.132.3.1027-1030.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Shinoda S., Honda T., Takeda Y., Miwatani T. Antigenic difference between polar montrichous and peritrichous flagella of Vibrio parahaemolyticus. J Bacteriol. 1974 Nov;120(2):923–928. doi: 10.1128/jb.120.2.923-928.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Shinoda S., Miwatani T., Fujino T. Antigens of Vibrio parahaemolyticus. II. Existence of two different subunits in the flagella of Vibrio parahaemolyticus and their characterization. Biken J. 1970 Dec;13(4):241–247. [PubMed] [Google Scholar]
- Shinoda S., Nakahara N. Antigenicity of lateral flagella of Vibrio parahaemolyticus: existence of two distinct antigenic determinants in a flagellum. Infect Immun. 1977 Jul;17(1):235–237. doi: 10.1128/iai.17.1.235-237.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Shinoda S., Okamoto K. Formation and function of Vibrio parahaemolyticus lateral flagella. J Bacteriol. 1977 Mar;129(3):1266–1271. doi: 10.1128/jb.129.3.1266-1271.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Yabuuchi E., Miwatani T., Takeda Y., Arita M. Flagellar morphology of Vibrio parahaemolyticus (Fujino et al) Sakazaki, Iwanami and Fukumi 1963. Jpn J Microbiol. 1974 Jul;18(4):295–305. doi: 10.1111/j.1348-0421.1974.tb00813.x. [DOI] [PubMed] [Google Scholar]