Skip to main content
PMC home page
Infection and Immunity logoLink to Infection and Immunity
. 1979 Nov;26(2):391–396. doi: 10.1128/iai.26.2.391-396.1979

Human complement activation by lipopolysaccharides from bacteroides oralis, fusobacterium nucleatum, and veillonella parvula.

H Nygren, G Dahlén, L A Nilsson
PMCID: PMC414626  PMID: 121108

Abstract

The properties of different lipopolysaccharide (LPS) preparations to induce C3 conversion in human serum was studied by means of crossed immunoelectrophoresis. C3 conversion by the alternative pathway was evaluated after calcium depletion, and lipid A-dependent activation was measured by means of inhibition with polymyxin B sulfate. LPS from Bacteroides oralis converted Co mainly via the alternative pathway, whereas LPS from Fusobacterium nucleatum and Veillonella parvula const pronounced lipid A-dependent conversion. The results are discussed in relation to the chemical composition of the LPS preparations.

Full text

PDF
391

Images in this article

393Image
on p.393
394Image
on p.394
394Image
on p.394

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bito L. Z. Inflammatory effects of endotoxin-like contaminants in commonly used protein preparations. Science. 1977 Apr 1;196(4285):83–85. doi: 10.1126/science.557238. [DOI] [PubMed] [Google Scholar]
  2. Bryant R. E., Jenkins D. E., Jr Calcium requirements for complement dependent hemolytic reactions. J Immunol. 1968 Oct;101(4):664–668. [PubMed] [Google Scholar]
  3. Dahlén G., Hofstad T. Endotoxic activities of lipopolysaccharides of microorganisms isolated from an infected root canal in Macaca cynomolgus. Scand J Dent Res. 1977 May;85(4):272–278. doi: 10.1111/j.1600-0722.1977.tb00563.x. [DOI] [PubMed] [Google Scholar]
  4. Dahlén G., Nygren H., Hannsson H. A. Immunoelectron microscopic localization of lipopolysaccharides in the cell wall of Bacteroides oralis and Fusobacterium nucleatum. Infect Immun. 1978 Jan;19(1):265–271. doi: 10.1128/iai.19.1.265-271.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Fine D. P. Activation of the classic and alternate complement pathways by endotoxin. J Immunol. 1974 Feb;112(2):763–769. [PubMed] [Google Scholar]
  6. Frank M. M., May J. E., Kane M. A. Contributions of the classical and alternate complement pathways to the biological effects of endotoxin. J Infect Dis. 1973 Jul;128(Suppl):176–181. doi: 10.1093/infdis/128.supplement_1.s176. [DOI] [PubMed] [Google Scholar]
  7. Galanos C., Rietschel E. T., Lüderitz O., Westphal O. Interaction of lipopolysaccharides and lipid A with complement. Eur J Biochem. 1971 Mar 1;19(1):143–152. doi: 10.1111/j.1432-1033.1971.tb01298.x. [DOI] [PubMed] [Google Scholar]
  8. Gewurz H., Shin H. S., Mergenhagen S. E. Interactions of the complement system with endotoxic lipopolysaccharide: consumption of each of the six terminal complement components. J Exp Med. 1968 Nov 1;128(5):1049–1057. doi: 10.1084/jem.128.5.1049. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Götze O., Müller-Eberhard H. J. The alternative pathway of complement activation. Adv Immunol. 1976;24:1–35. doi: 10.1016/s0065-2776(08)60328-4. [DOI] [PubMed] [Google Scholar]
  10. Hawley C. E., Falkler W. A., Jr Anticomplementary activity of Fusobacterium polymorphum in normal and C4-deficient sources of guinea pig complement. Infect Immun. 1977 Oct;18(1):124–129. doi: 10.1128/iai.18.1.124-129.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Hofstad T. Antibodies reacting with lipopolysaccharides from Bacteroides melaninogenicus, in serum from normal human subjects. J Infect Dis. 1974 Mar;129(3):349–352. doi: 10.1093/infdis/129.3.349. [DOI] [PubMed] [Google Scholar]
  12. Jensen J. A. A specific inactivator of mammalian C'4 isolated from nurse shark (Ginglymostoma cirratum) serum. J Exp Med. 1969 Aug 1;130(2):217–241. doi: 10.1084/jem.130.2.217. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. LAURELL C. B. ANTIGEN-ANTIBODY CROSSED ELECTROPHORESIS. Anal Biochem. 1965 Feb;10:358–361. doi: 10.1016/0003-2697(65)90278-2. [DOI] [PubMed] [Google Scholar]
  14. Linder L., Holme T., Frostell G. Hyaluronidase and aminopeptidase activity in cultures of streptococcus mitis, ATCC 903. Acta Pathol Microbiol Scand B Microbiol Immunol. 1974 Aug;82(4):521–526. doi: 10.1111/j.1699-0463.1974.tb02360.x. [DOI] [PubMed] [Google Scholar]
  15. Mergenhagen S. E., Snyderman R., Gewurz H., Shin H. S. Significance of complement to the mechanism of action of endotoxin. Curr Top Microbiol Immunol. 1969;50:37–77. doi: 10.1007/978-3-642-46169-9_2. [DOI] [PubMed] [Google Scholar]
  16. Mergenhagen S. E., Snyderman R., Phillips J. K. Activation of complement by endotoxin. J Infect Dis. 1973 Jul;128(Suppl):86–90. doi: 10.1093/infdis/128.supplement_1.s86. [DOI] [PubMed] [Google Scholar]
  17. Morrison D. C., Jacobs D. M. Binding of polymyxin B to the lipid A portion of bacterial lipopolysaccharides. Immunochemistry. 1976 Oct;13(10):813–818. doi: 10.1016/0019-2791(76)90181-6. [DOI] [PubMed] [Google Scholar]
  18. Morrison D. C., Jacobs D. M. Inhibition of lipopolysaccharide-initiated activation of serum complement by polymyxin B. Infect Immun. 1976 Jan;13(1):298–301. doi: 10.1128/iai.13.1.298-301.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Morrison D. C., Kline L. F. Activation of the classical and properdin pathways of complement by bacterial lipopolysaccharides (LPS). J Immunol. 1977 Jan;118(1):362–368. [PubMed] [Google Scholar]
  20. Morrison D. C., Kline L. F., Oades Z. G., Henson P. M. Mechanisms of lipopolysaccharide-initiated rabbit platelet responses: alternative complement pathway dependence of the lytic response. Infect Immun. 1978 Jun;20(3):744–751. doi: 10.1128/iai.20.3.744-751.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. NETER E., WESTPHAL O., LUDERITZ O., GORZYNSKI E. A., EICHENBERGER E. Studies of enterobacterial lipopolysaccharides; effects of heat and chemicals on erythrocyte-modifying, antigenic, toxic and pyrogenic properties. J Immunol. 1956 May;76(5):377–385. [PubMed] [Google Scholar]
  22. Platts-Mills T. A., Ishizaka K. Activation of the alternate pathway of human complements by rabbit cells. J Immunol. 1974 Jul;113(1):348–358. [PubMed] [Google Scholar]
  23. Snyderman R., Pike M. C. Interaction of complex polysaccharides with the complement system: effect of calcium depletion on terminal component consumption. Infect Immun. 1975 Feb;11(2):273–279. doi: 10.1128/iai.11.2.273-279.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Socransky S. S. Relationship of bacteria to the etiology of periodontal disease. J Dent Res. 1970 Mar-Apr;49(2):203–222. doi: 10.1177/00220345700490020401. [DOI] [PubMed] [Google Scholar]
  25. Sveen K. The importance of C5 and the role of the alternative complement pathway in leukocyte chemotaxis induced in vivo and in vitro by Bacteroides fragilis lipopolysaccharide. Acta Pathol Microbiol Scand B. 1978 Apr;86(2):93–100. doi: 10.1111/j.1699-0463.1978.tb00015.x. [DOI] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES