Skip to main content
NCBI home page
Infection and Immunity logoLink to Infection and Immunity
. 1979 Nov;26(2):402–407. doi: 10.1128/iai.26.2.402-407.1979

Effect of iron on surface charge and hydrophobicity of Neisseria gonorrhoeae.

K E Magnusson, E Kihlstrom, A Norqvist, J Davies, S Normark
PMCID: PMC414628  PMID: 44700

Abstract

The effect of iron concentration during growth on the physicochemical surface properties of the colonial variants of Neisseria gonorrhoeae has been assessed by aqueous two-phase partitioning in a dextran-polyethyleneglycol system containing positively charged trimethylamino-polyethyleneglycol or hydrophobic polyethyleneglycol-palmitate. The complex effects of iron, in combination with other variables known to affect surface charge and hydrophobicity, have provided some clues as to the properties of the gonococcal surface that are important in promoting virulence.

Full text

PDF
402

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bumgarner L. R., Finkelstein R. A. Pathogenesis and immunology of experimental gonococcal infection: virulence of colony types of Neisseria gonorrhoeae for chicken embryos. Infect Immun. 1973 Dec;8(6):919–924. doi: 10.1128/iai.8.6.919-924.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Elmros T., Hörstedt P., Winblad B. Scanning electron microscopic study of virulent and avirulent colonies of Neisseria gonorrhoeae. Infect Immun. 1975 Sep;12(3):630–637. doi: 10.1128/iai.12.3.630-637.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Hebeler B. H., Morse S. A., Wong W., Young F. E. Evidence for peptidoglycan-associated protein(s) in Neisseria gonorrhoeae. Biochem Biophys Res Commun. 1978 Apr 14;81(3):1011–1017. doi: 10.1016/0006-291x(78)91451-1. [DOI] [PubMed] [Google Scholar]
  4. Jephcott A. E., Reyn A., Birch-Andersen A. Neisseria gonorrhoeae 3. Demonstration of presumed appendages to cells from different colony types. Acta Pathol Microbiol Scand B Microbiol Immunol. 1971;79(3):437–439. doi: 10.1111/j.1699-0463.1971.tb00086.x. [DOI] [PubMed] [Google Scholar]
  5. Johansson G. Studies on aqueous dextran-poly (ethylene glycol) two-phase systems containing charged poly (ethylene glycol). I. Partition of albumins. Biochim Biophys Acta. 1970 Nov 24;222(2):381–389. doi: 10.1016/0304-4165(70)90127-3. [DOI] [PubMed] [Google Scholar]
  6. Johansson G. The effect of poly(ethyleneglycol) esters on the partition of proteins and fragmented membranes in aqueous biphasic systems. Biochim Biophys Acta. 1976 Dec 21;451(2):517–529. doi: 10.1016/0304-4165(76)90147-1. [DOI] [PubMed] [Google Scholar]
  7. Johnston K. H., Holmes K. K., Gotschlich E. C. The serological classification of Neisseria gonorrhoeae. I. Isolation of the outer membrane complex responsible for serotypic specificity. J Exp Med. 1976 Apr 1;143(4):741–758. doi: 10.1084/jem.143.4.741. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. KELLOGG D. S., Jr, PEACOCK W. L., Jr, DEACON W. E., BROWN L., PIRKLE D. I. NEISSERIA GONORRHOEAE. I. VIRULENCE GENETICALLY LINKED TO CLONAL VARIATION. J Bacteriol. 1963 Jun;85:1274–1279. doi: 10.1128/jb.85.6.1274-1279.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Kellogg D. S., Jr, Cohen I. R., Norins L. C., Schroeter A. L., Reising G. Neisseria gonorrhoeae. II. Colonial variation and pathogenicity during 35 months in vitro. J Bacteriol. 1968 Sep;96(3):596–605. doi: 10.1128/jb.96.3.596-605.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Magnusson K. E., Kihlström E., Norlander L., Norqvist A., Davies J., Normark S. Effect of colony type and pH on surface charge and hydrophobicity of Neisseria gonorrhoeae. Infect Immun. 1979 Nov;26(2):397–401. doi: 10.1128/iai.26.2.397-401.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Magnusson K. E., Stendahl O., Tagesson C., Edebo L., Johansson G. The tendency of smooth and rough Salmonella typhimurium bacteria and lipopolysaccharide to hydrophobic and ionic interaction, as studied in aqueous polymer two-phase systems. Acta Pathol Microbiol Scand B. 1977 Jun;85(3):212–218. doi: 10.1111/j.1699-0463.1977.tb01698.x. [DOI] [PubMed] [Google Scholar]
  12. Mayer L. W., Holmes K. K., Falkow S. Characterization of plasmid deoxyribonucleic acid from Neisseria gonorrhoeae. Infect Immun. 1974 Oct;10(4):712–717. doi: 10.1128/iai.10.4.712-717.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Payne S. M., Finkelstein R. A. Pathogenesis and immunology of experimental gonococcal infection: role of iron in virulence. Infect Immun. 1975 Dec;12(6):1313–1318. doi: 10.1128/iai.12.6.1313-1318.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Payne S. M., Finkelstein R. A. The critical role of iron in host-bacterial interactions. J Clin Invest. 1978 Jun;61(6):1428–1440. doi: 10.1172/JCI109062. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Salit I. E., Gotschlich E. C. Gonococcal color and opacity variants: virulence for chicken embryos. Infect Immun. 1978 Nov;22(2):359–364. doi: 10.1128/iai.22.2.359-364.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Swanson J. Studies on gonococcus infection. XIV. Cell wall protein differences among color/opacity colony variants of Neisseria gonorrhoeae. Infect Immun. 1978 Jul;21(1):292–302. doi: 10.1128/iai.21.1.292-302.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Tagesson C., Stendahl O. Influence of the cell surface lipopolysaccharide structure of Salmonella typhimurium on resistance to intracellular bactericidal systems. Acta Pathol Microbiol Scand B Microbiol Immunol. 1973 Aug;81(4):473–480. doi: 10.1111/j.1699-0463.1973.tb02232.x. [DOI] [PubMed] [Google Scholar]
  18. Weinberg E. D. Iron and infection. Microbiol Rev. 1978 Mar;42(1):45–66. doi: 10.1128/mr.42.1.45-66.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Wolf-Watz H., Elmros T., Normark S., Bloom G. D. Cell envelope of Neisseria gonorrhoeae: outer membrane and peptidoglycan composition of penicillin-sensitive and-resistant strains. Infect Immun. 1975 Jun;11(6):1332–1341. doi: 10.1128/iai.11.6.1332-1341.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES