Abstract
Different methods were used to assay diphtheria toxin sensitivity of fibroblast and heart cell cultures from chicken embryos of various ages. As defined by inhibition of protein synthesis, fibroblasts from 18-day and younger embryos respond more rapidly to toxin than fibroblasts from older embryos. The response of heart cells cultures is independent of the age of the embryos and is similar to the response of fibroblasts from 18-day embryos. Since the EF-2 content is 10-fold less in fibroblasts from 21-day-old embryos, the different responses of protein synthesis to intoxication appear to reside at the membrane level. Cytotoxicity assays in cell culture and in vivo toxin sensitivity assays show that cells from both young and old embryos, as well as whole embryos, are equally sensitive to toxin. Thus, short-term (5-h) measurements of inhibition of protein synthesis are insufficient for determining the relative sensitivity of cells to intoxication as defined by cell death.
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Selected References
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- Bonventre P. F., Imhoff J. G. Studies on the mode of action of diphtheria toxin. I. Protein synthesis in guinea pig tissues. J Exp Med. 1966 Dec 1;124(6):1107–1122. doi: 10.1084/jem.124.6.1107. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Collier R. J. Diphtheria toxin: mode of action and structure. Bacteriol Rev. 1975 Mar;39(1):54–85. doi: 10.1128/br.39.1.54-85.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Collier R. J., Kandel J. Structure and activity of diphtheria toxin. I. Thiol-dependent dissociation of a fraction of toxin into enzymically active and inactive fragments. J Biol Chem. 1971 Mar 10;246(5):1496–1503. [PubMed] [Google Scholar]
- GABLIKS J., SOLOTOROVSKY M. Cell culture reactivity to diphtheria, Staphylococcus, tetanus and Escherichia coli toxins. J Immunol. 1962 Apr;88:505–512. [PubMed] [Google Scholar]
- Gill D. M., Dinius L. L. Observations on the structure of diphtheria toxin. J Biol Chem. 1971 Mar 10;246(5):1485–1491. [PubMed] [Google Scholar]
- Hanafusa H. Rapid transformation of cells by Rous sarcoma virus. Proc Natl Acad Sci U S A. 1969 Jun;63(2):318–325. doi: 10.1073/pnas.63.2.318. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Iglewski B. H., Rittenberg M. B., Iglewski W. J. Preferential inhibition of growth and protein synthesis in Rous sarcoma virus transformed cells by diphtheria toxin. Virology. 1975 May;65(1):272–275. doi: 10.1016/0042-6822(75)90029-x. [DOI] [PubMed] [Google Scholar]
- Murphy J. R., Bacha P., Teng M. Determination of Corynebacterium diphtheriae toxigenicity by a colorimetric tissue culture assay. J Clin Microbiol. 1978 Jan;7(1):91–96. doi: 10.1128/jcm.7.1.91-96.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Pappenheimer A. M., Jr Diphtheria toxin. Annu Rev Biochem. 1977;46:69–94. doi: 10.1146/annurev.bi.46.070177.000441. [DOI] [PubMed] [Google Scholar]
- Schneider H., Shaw M. W., Muirhead E. E., Smith A. The in vitro culture of embryonic chicken heart cells. Exp Cell Res. 1965 Sep;39(2):631–636. doi: 10.1016/0014-4827(65)90066-2. [DOI] [PubMed] [Google Scholar]
- Taylor A., Iglewski W. J. Intoxication of normal and virus-transformed cells by diphtheria toxin. Virology. 1979 Jul 15;96(1):177–185. doi: 10.1016/0042-6822(79)90183-1. [DOI] [PubMed] [Google Scholar]