Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2014 Jul;141(13):2633–2643. doi: 10.1242/dev.101956

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2014. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

PMC Copyright notice

Fig. 1. — Tramtrack69 is expressed in the somatic and visceral mesoderm of Drosophila embryos. (A) Binding of five mesodermal TFs in the ttk locus at 6-8 h after egg laying [data from Zinzen et al. (2009)]. Genes are depicted at the bottom, the ttk-VME CRM is in gray, the asterisk marks a recently annotated gene (see http://flybase.org/reports/FBrf0204466.html). (B,C) Immunostaining against the GFP reporter driven by the ttk-VME enhancer (green). (B) CRM activity overlaps expression of endogenous Ttk69 protein (magenta) in mesoderm (arrows) and in tracheal placodes (arrowhead). (C) ttk-VME is active in the visceral mesoderm (VM) and in a subset of the somatic mesoderm (SM) (arrows), overlapping with expression of Mef2 protein (magenta). (D,E) Fluorescent in situ hybridization against endogenous ttk transcripts (green) and the mesodermal marker Mef2 (magenta). (D) In SM (arrows), ttk is expressed transiently at stage 11. (E) At stage 12, there is no detectable ttk expression in SM. (F,G) Double immunostaining using a Ttk69-specific antibody (green) and anti-Bin (magenta), marking the VM. (F) At stage 11, Ttk69 is robustly expressed in VM, where it colocalizes with Bin (arrow), and in endoderm (arrowhead). (G) At stage 16, Ttk69 is expressed in both VM (arrows) and endoderm (arrowhead).