Abstract
The effect of mouse-adapted influenza A/PR/8/34 virus on pulmonary macrophage function was evaluated by using an in vitro system which allowed direct virus interaction with macrophages and then separate analysis of the steps required for bacterial clearance by macrophages. Infection of macrophages with this virus resulted in the appearance of a hemagglutinating activity on the macrophage surface; expression of this activity was inhibited by amantadine, 2-deoxyglucose, and cycloheximide and by pretreatment of the virus inoculum with ultraviolet light and specific antiserum. Since there was no release of extracellular virus, this growth cycle appeared to be incomplete (abortive). After influenza infection, net ingestion of viable Staphylococcus aureus by macrophage monolayers was unaltered and there was no change in the fraction of the monolayer which ingested cocci over a wide range of bacterial inputs. Influenza-infected macrophages also inactivated intracellular S. aureus at a rate indistinguishable from controls. Therefore, these in vitro studies do not support the hypothesis that the defect in pulmonary antibacterial mechanisms associated with influenza infections results from a direct effect of virus infection on either the phagocytic or bactericidal activity of resident pulmonary macrophages.
Full text
PDF






Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Anismová E., Tucková E., Vonka V., Závadová H. Ultrastructural changes induced by influenza viruses in permissive and nonpermissive cells. Virology. 1977 Mar;77(1):330–336. doi: 10.1016/0042-6822(77)90429-9. [DOI] [PubMed] [Google Scholar]
- Baughn R., Bonventre P. F. Phagocytosis and intracellular killing of Staphylococcus aureus by normal mouse peritoneal macrophages. Infect Immun. 1975 Aug;12(2):346–352. doi: 10.1128/iai.12.2.346-352.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Brain J. D., Frank R. Alveolar macrophage adhesion: wash electrolyte composition and free cell yield. J Appl Physiol. 1973 Jan;34(1):75–80. doi: 10.1152/jappl.1973.34.1.75. [DOI] [PubMed] [Google Scholar]
- COHN Z. A., MORSE S. I. Interactions between rabbit polymorphonuclear leucocytes and staphylococci. J Exp Med. 1959 Sep 1;110:419–443. doi: 10.1084/jem.110.3.419. [DOI] [PMC free article] [PubMed] [Google Scholar]
- FAZEKAS DE ST GROTH S., WHITE D. O. An improved assay for the infectivity of in influenza viruses. J Hyg (Lond) 1958 Mar;56(1):151–162. doi: 10.1017/s0022172400037621. [DOI] [PMC free article] [PubMed] [Google Scholar]
- GREEN G. M., KASS E. H. THE ROLE OF THE ALVEOLAR MACROPHAGE IN THE CLEARANCE OF BACTERIA FROM THE LUNG. J Exp Med. 1964 Jan 1;119:167–176. doi: 10.1084/jem.119.1.167. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Gandhi S. S., Bell H. B., Burke D. C. Abortive infection of L cells by fowl plague virus: comparison of RNA and protein synthesis in infected chick and L cells. J Gen Virol. 1971 Dec;13(3):424–432. doi: 10.1099/0022-1317-13-3-423. [DOI] [PubMed] [Google Scholar]
- Jackson G. G., Muldoon R. L. Viruses causing common respiratory infections in man. V. influenza A (Asian). J Infect Dis. 1975 Mar;131(3):308–357. doi: 10.1093/infdis/131.3.308. [DOI] [PubMed] [Google Scholar]
- Jakab G. J., Green G. M. Defect in intracellular killing of Staphylococcus aureus within alveolar macrophages in Sendai virus-infected murine lungs. J Clin Invest. 1976 Jun;57(6):1533–1539. doi: 10.1172/JCI108423. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Jakab G. J., Green G. M. The effect of Sendai virus infection on bactericidal and transport mechanisms of the murine lung. J Clin Invest. 1972 Aug;51(8):1989–1998. doi: 10.1172/JCI107005. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kleinerman E. S., Daniels C. A., Polisson R. P., Snyderman R. Effect of virus infection on the inflammatory response. Depression of macrophage accumulation in influenza-infected mice. Am J Pathol. 1976 Nov;85(2):373–382. [PMC free article] [PubMed] [Google Scholar]
- LaForce F. M., Kelly W. J., Huber G. L. Inactivation of staphylococci by alveolar macrophages with preliminary observations on the importance of alveolar lining material. Am Rev Respir Dis. 1973 Oct;108(4):784–790. doi: 10.1164/arrd.1973.108.4.784. [DOI] [PubMed] [Google Scholar]
- Pesanti E. L. Suramin effects on macrophage phagolysosome formation and antimicrobial activity. Infect Immun. 1978 May;20(2):503–511. doi: 10.1128/iai.20.2.503-511.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Shayegani M., Lief F. S., Mudd S. Specific and nonspecific cell-mediated resistance to influenza virus in mice. Infect Immun. 1974 Jun;9(6):991–998. doi: 10.1128/iai.9.6.991-998.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
- VOGEL J., SHELOKOV A. Adsorption-hemagglutination test for influenza virus in monkey kidney tissue culture. Science. 1957 Aug 23;126(3269):358–359. doi: 10.1126/science.126.3269.358-a. [DOI] [PubMed] [Google Scholar]
- Warshauer D., Goldstein E., Akers T., Lippert W., Kim M. Effect of influenza viral infection on the ingestion and killing of bacteria by alveolar macrophages. Am Rev Respir Dis. 1977 Feb;115(2):269–277. doi: 10.1164/arrd.1977.115.2.269. [DOI] [PubMed] [Google Scholar]
- Wells M. A., Albrecht P., Daniel S., Ennis F. A. Host defense mechanisms against influenza virus: interaction of influenza virus with murine macrophages in vitro. Infect Immun. 1978 Dec;22(3):758–762. doi: 10.1128/iai.22.3.758-762.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wyde P. R., Couch R. B., Mackler B. F., Cate T. R., Levy B. M. Effects of low- and high-passage influenza virus infection in normal and nude mice. Infect Immun. 1977 Jan;15(1):221–229. doi: 10.1128/iai.15.1.221-229.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]