Abstract
Antigens of Fasciola hepatica adult worms were chromatographed using concanavalin A-Sepharose 4B. Two unbound peaks appeared in the inclusion volume (DT-1 and DT-2), and one peak was eluted with alpha-methylglucoside (E1-1). At least seven peaks were obtained by isoelectric focusing of E1-1. The largest of these peaks, with an average pI of 4.0, contained the antigens reactive with antibodies to Schistosoma mansoni. Mice immunized with DT-2 or E1-1 and challenged with S. mansoni cercariae developed 39 to 82% fewer worms than controls. DT-1 had no protective effect. Combining DT-1 and DT-2 abolished this protection. These experiments demonstrate that F. hepatica glycoprotein antigens induce in mice significant protection to infection with S. mansoni and offer an interesting approach to the study of vaccines in experimental schistosomiasis.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Hillyer G. V. Can we vaccinate against schistosomes? Fed Proc. 1976 Dec;35(14):2568–2571. [PubMed] [Google Scholar]
- Hillyer G. V., Cervoni M. Laurell crossed immunoelectrophoresis and affinity chromatography for the purification of a parasite antigen. J Immunol Methods. 1978;20:385–390. doi: 10.1016/0022-1759(78)90273-9. [DOI] [PubMed] [Google Scholar]
- Hillyer G. V., Pelley R. P., del Llano de Diaz A. Solubilization of antigens of Fasciola hepatica which react with antibodies to Schistosoma mansoni. J Parasitol. 1979 Feb;65(1):55–60. [PubMed] [Google Scholar]
- Hillyer G. V., del Llano de Díaz A., García-Blanco M. A new approach to the study of immunity to schistosomes. Bol Asoc Med P R. 1975 Dec;67(12):381–385. [PubMed] [Google Scholar]
- Hillyer G. V., del Llano de Díaz A., Reyes C. N. Schistosoma mansoni: acquired immunity in mice and hamsters using antigens of Fasciola hepatica. Exp Parasitol. 1977 Aug;42(2):348–355. doi: 10.1016/0014-4894(77)90092-3. [DOI] [PubMed] [Google Scholar]
- Maddison S. E., Slemenda S. B., Chandler F. W., Kagan I. G. Studies on putative adult worm-derived vaccines and adjuvants for protection against Schistosoma mansoni infection in mice. J Parasitol. 1978 Dec;64(6):986–993. [PubMed] [Google Scholar]