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. 1979 Dec;26(3):910–915. doi: 10.1128/iai.26.3.910-915.1979

Phagocytosis-induced injury of normal and activated alveolar macrophages.

M P McGee, Q N Myrvik
PMCID: PMC414706  PMID: 231011

Abstract

The present study tested the hypothesis that BCG-activated macrophages become injured when they phagocytose certain particulates. The data indicate that alveolar macrophages obtained from Mycobacterium bovis BCG-sensitized animals were more susceptible to cell death after in vitro incubation with BCG or zymosan than were macrophages from normal animals. Increased susceptibility was dependent on phagocytosis, since incubation with cytochalasin B, a phagocytosis inhibitor, abrogated the effect. Catalase, cytochrome c, and ascorbic acid offered partial protection to the macrophage, suggesting the involvement of free radicals in the generation of cytotoxicity. Not all of the cells from the alveolar populations were equally susceptible to cell death, thus suggesting either heterogeneity in the cell population or a requirement of more than one cell type in the induction of necrosis or both.

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Selected References

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  1. Adams D. O. The granulomatous inflammatory response. A review. Am J Pathol. 1976 Jul;84(1):164–192. [PMC free article] [PubMed] [Google Scholar]
  2. Boros D. L. Granulomatous inflammations. Prog Allergy. 1978;24:183–267. doi: 10.1159/000401230. [DOI] [PubMed] [Google Scholar]
  3. Dannenberg A. M., Jr, Sugimoto M. Liquefaction of caseous foci in tuberculosis. Am Rev Respir Dis. 1976 Mar;113(3):257–259. doi: 10.1164/arrd.1976.113.3.257. [DOI] [PubMed] [Google Scholar]
  4. David J. R., David R. R. Cellular hypersensitivity and immunity. Inhibition of macrophage migration and the lymphocyte mediators. Prog Allergy. 1972;16:300–449. [PubMed] [Google Scholar]
  5. MYRVIK Q., LEAKE E. S., FARISS B. Studies on pulmonary alveolar macrophages from the normal rabbit: a technique to procure them in a high state of purity. J Immunol. 1961 Feb;86:128–132. [PubMed] [Google Scholar]
  6. Malawista S. E., Gee J. B., Bensch K. G. Cytochalasin B reversibly inhibits phagocytosis: functional, metabolic, and ultrastructural effects in human blood leukocytes and rabbit alveolar macrophages. Yale J Biol Med. 1971 Dec;44(3):286–300. [PMC free article] [PubMed] [Google Scholar]
  7. Marx J. J., Jr, Burrell R. Delayed hypersensitivity to beryllium compounds. J Immunol. 1973 Aug;111(2):590–598. [PubMed] [Google Scholar]
  8. McGee M. P., Myrvik Q. N. Hydrolase levels in necrotizing and non-necrotizing BCG-induced pulmonary granulomas. J Reticuloendothel Soc. 1976 Sep;20(3):187–195. [PubMed] [Google Scholar]
  9. McGee M. P., Myrvik Q. N., Leake E. S. Organization of allergic granulomas and dependence on insoluble antigen. J Reticuloendothel Soc. 1978 Sep;24(3):253–262. [PubMed] [Google Scholar]
  10. Mustafa M. G., Tierney D. F. Biochemical and metabolic changes in the lung with oxygen, ozone, and nitrogen dioxide toxicity. Am Rev Respir Dis. 1978 Dec;118(6):1061–1090. doi: 10.1164/arrd.1978.118.6.1061. [DOI] [PubMed] [Google Scholar]
  11. Nathan C. F., Silverstein S. C., Brukner L. H., Cohn Z. A. Extracellular cytolysis by activated macrophages and granulocytes. II. Hydrogen peroxide as a mediator of cytotoxicity. J Exp Med. 1979 Jan 1;149(1):100–113. doi: 10.1084/jem.149.1.100. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Preston P. M., Hart P. D., Brown I. N. Immunologically mediated macrophage aggregation in monolayers of peritoneal cells from BCG-sensitized mice. Immunology. 1977 Jan;32(1):33–41. [PMC free article] [PubMed] [Google Scholar]
  13. Salin M. L., McCord J. M. Free radicals and inflammation. Protection of phagocytosine leukocytes by superoxide dismutase. J Clin Invest. 1975 Nov;56(5):1319–1323. doi: 10.1172/JCI108208. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Taplits M. S., Myrvik Q. N. Host resistance to the LVS strain of Francisella tularensis in BCG vaccinated mice. J Reticuloendothel Soc. 1977 Oct;22(4):297–308. [PubMed] [Google Scholar]
  15. Yamamura Y., Ogawa Y., Maeda H., Yamamura Y. Prevention of tuberculous cavity formation by desensitization with tuberculin-active peptide. Am Rev Respir Dis. 1974 Jun;109(6):594–601. doi: 10.1164/arrd.1974.109.6.594. [DOI] [PubMed] [Google Scholar]

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