Skip to main content
NCBI home page
Infection and Immunity logoLink to Infection and Immunity
. 1979 Dec;26(3):1004–1008. doi: 10.1128/iai.26.3.1004-1008.1979

Neutrophil chemotaxis in patients with Staphylococcus aureus furunculosis.

K L Cates, P G Quie
PMCID: PMC414719  PMID: 393626

Abstract

Neutrophil chemotaxis was evaluated in patients with staphylococcal furunculosis using a modified Boyden chamber assay. Neutrophil chemotactic response to Staphylococcus aureus-derived chemotactic factor was compared with response to Escherichia coli-derived chemotactic factor and zymosan-activated serum. Twenty-one patients with active furunculosis were compared with 29 patients with a history of furunculosis but no recent infection and with 29 healthy control subjects. Chemotactic response to the staphylococcal chemotactic factor was significantly higher in patients with active furunculosis (mean 61.6) than in patients with a history of furunculosis (mean 36.4) or controls (mean 31.4), P less than 0.001. Neutrophils from patients with active staphylococcal infections also had higher chemotactic activity toward E. coli chemotactic factor, but not significantly so (P = 0.09). Chemotactic response to zymosan-activated serum and background neutrophil motility was comparable among the three groups. The increased neutrophil chemotactic response of patients with active infection to bacterial factors, but not zymosan-activated serum, may represent a specific neutrophil response to products of infecting organisms. The differential response of the patients' neutrophils to these attractants supports evidence for the presence of separate categories of chemotaxin receptor on the surface of neutrophils.

Full text

PDF
1004

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aswanikumar S., Corcoran B., Schiffmann E., Day A. R., Freer R. J., Showell H. J., Becker E. L. Demonstration of a receptor on rabbit neutrophils for chemotactic peptides. Biochem Biophys Res Commun. 1977 Jan 24;74(2):810–817. doi: 10.1016/0006-291x(77)90375-8. [DOI] [PubMed] [Google Scholar]
  2. Blume R. S., Wolff S. M. The Chediak-Higashi syndrome: studies in four patients and a review of the literature. Medicine (Baltimore) 1972 Jul;51(4):247–280. [PubMed] [Google Scholar]
  3. Chenoweth D. E., Erickson B. W., Hugli T. E. Human C5a-related synthetic peptides as neutrophil chemotactic factors. Biochem Biophys Res Commun. 1979 Jan 30;86(2):227–234. doi: 10.1016/0006-291x(79)90856-8. [DOI] [PubMed] [Google Scholar]
  4. Craig C. P., Normann S. J., McGann V., Irvin W. S. Chemotactic activity generated by staphylococcal enterotoxin B. Infect Immun. 1971 Jan;3(1):94–99. doi: 10.1128/iai.3.1.94-99.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Grov A., Sveen K. Induction of leukochemotaxis by peptidoglycan of Staphylococcus aureus. Acta Pathol Microbiol Scand B. 1978 Dec;86B(6):375–378. doi: 10.1111/j.1699-0463.1978.tb00059.x. [DOI] [PubMed] [Google Scholar]
  6. Harvey R. L., Kronvall G., Troup G. M., Anderson R. E., Williams R. C., Jr Chemotaxis of polymorphonuclear leukocytes by protein A of the staphylococcus. Proc Soc Exp Biol Med. 1970 Nov;135(2):453–456. doi: 10.3181/00379727-135-35073. [DOI] [PubMed] [Google Scholar]
  7. Hill H. R., Gerrard J. M., Hogan N. A., Quie P. G. Hyperactivity of neutrophil leukotactic responses during active bacterial infection. J Clin Invest. 1974 Apr;53(4):996–1002. doi: 10.1172/JCI107666. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Russell R. J., Wilkinson P. C., McInroy R. J., McKay S., McCartney A. C., Arbuthnott J. P. Effects of staphylococcal products on locomotion and chemotaxis of human blood neutrophils and monocytes. J Med Microbiol. 1976 Nov;9(4):433–439. doi: 10.1099/00222615-9-4-433. [DOI] [PubMed] [Google Scholar]
  9. Schmeling D. J., Peterson P. K., Hammerschmidt D. E., Kim Y., Verhoef J., Wilkinson B. J., Quie P. G. Chemotaxigenesis by cell surface components of Staphylococcus aureus. Infect Immun. 1979 Oct;26(1):57–63. doi: 10.1128/iai.26.1.57-63.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Sveen K., Grov A. Induction of leukochemotaxis by protein A of Staphylococcus aureus. Acta Pathol Microbiol Scand B. 1978 Dec;86B(6):369–373. doi: 10.1111/j.1699-0463.1978.tb00058.x. [DOI] [PubMed] [Google Scholar]
  11. Walker W. S., Barlet R. L., Kurtz H. M. Isolation and partial characterization of a staphylococcal leukocyte cytotaxin. J Bacteriol. 1969 Mar;97(3):1005–1008. doi: 10.1128/jb.97.3.1005-1008.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Ward P. A., Lepow I. H., Newman L. J. Bacterial factors chemotactic for polymorphonuclear leukocytes. Am J Pathol. 1968 Apr;52(4):725–736. [PMC free article] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES