Skip to main content
PMC home page
Infection and Immunity logoLink to Infection and Immunity
. 1979 Dec;26(3):1009–1013. doi: 10.1128/iai.26.3.1009-1013.1979

Respiratory syncytial virus infection in owl monkeys: viral shedding, immunological response, and associated illness caused by wild-type virus and two temperature-sensitive mutants.

G A Prince, S C Suffin, D A Prevar, E Camargo, D L Sly, W T London, R M Chanock
PMCID: PMC414720  PMID: 118925

Abstract

Intranasal inoculation of owl monkeys with wild-type respiratory syncytial virus induced upper respiratory tract disease in each of seven animals. The response of owl monkeys to two highly defective, temperature-sensitive, multiple-lesion mutants was then compared to the pattern seen with wild-type respiratory syncytial virus. These mutants, ts-1 NG-1 and ts-1 NG-16, were derived from the ts-1 mutant that had been remutagenized with nitrosoguanidine (NG). Previously the ts-1 NG-1 and ts-1 NG-16 mutants had been shown to be more temperature sensitive and more stable genetically than their ts-1 parent. Both ts-1 NG-1 and ts-1 NG-16 produced infection that was delayed in onsent compared to wild-type virus infection. However, the mutants were shed from the upper respiratory tract for the same period of time and at the same titer as wild-type virus. The serum neutralizing antibody response to infection with the mutants was nearly equivalent to that elicited by wild-type virus. However, the extent of disease induced by the mutants was significantly less than that seen with wild-type virus. These observations suggest that the mutants are potential vaccine condidates and should be subjected to additional in vivo testing in primates and, ultimately, humans.

Full text

PDF
1009

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Belshe R. B., Richardson L. S., London W. T., Sly D. L., Lorfeld J. H., Camargo E., Prevar D. A., Chanock R. M. Experimental respiratory syncytial virus infection of four species of primates. J Med Virol. 1977;1(3):157–162. doi: 10.1002/jmv.1890010302. [DOI] [PubMed] [Google Scholar]
  2. Chin J., Magoffin R. L., Shearer L. A., Schieble J. H., Lennette E. H. Field evaluation of a respiratory syncytial virus vaccine and a trivalent parainfluenza virus vaccine in a pediatric population. Am J Epidemiol. 1969 Apr;89(4):449–463. doi: 10.1093/oxfordjournals.aje.a120957. [DOI] [PubMed] [Google Scholar]
  3. Fulginiti V. A., Eller J. J., Sieber O. F., Joyner J. W., Minamitani M., Meiklejohn G. Respiratory virus immunization. I. A field trial of two inactivated respiratory virus vaccines; an aqueous trivalent parainfluenza virus vaccine and an alum-precipitated respiratory syncytial virus vaccine. Am J Epidemiol. 1969 Apr;89(4):435–448. doi: 10.1093/oxfordjournals.aje.a120956. [DOI] [PubMed] [Google Scholar]
  4. Gharpure M. A., Wright P. F., Chanock R. M. Temperature-sensitive mutants of respiratory syncytial virus. J Virol. 1969 Apr;3(4):414–421. doi: 10.1128/jvi.3.4.414-421.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Kapikian A. Z., Mitchell R. H., Chanock R. M., Shvedoff R. A., Stewart C. E. An epidemiologic study of altered clinical reactivity to respiratory syncytial (RS) virus infection in children previously vaccinated with an inactivated RS virus vaccine. Am J Epidemiol. 1969 Apr;89(4):405–421. doi: 10.1093/oxfordjournals.aje.a120954. [DOI] [PubMed] [Google Scholar]
  6. Kim H. W., Arrobio J. O., Brandt C. D., Wright P., Hodes D., Chanock R. M., Parrott R. H. Safety and antigenicity of temperature sensitive (TS) mutant respiratory syncytial virus (RSV) in infants and children. Pediatrics. 1973 Jul;52(1):56–63. [PubMed] [Google Scholar]
  7. Kim H. W., Canchola J. G., Brandt C. D., Pyles G., Chanock R. M., Jensen K., Parrott R. H. Respiratory syncytial virus disease in infants despite prior administration of antigenic inactivated vaccine. Am J Epidemiol. 1969 Apr;89(4):422–434. doi: 10.1093/oxfordjournals.aje.a120955. [DOI] [PubMed] [Google Scholar]
  8. Mills J., 5th, Van Kirk J. E., Wright P. F., Chanock R. M. Experimental respiratory syncytial virus infection of adults. Possible mechanisms of resistance to infection and illness. J Immunol. 1971 Jul;107(1):123–130. [PubMed] [Google Scholar]
  9. Prince G. A., Jenson A. B., Horswood R. L., Camargo E., Chanock R. M. The pathogenesis of respiratory syncytial virus infection in cotton rats. Am J Pathol. 1978 Dec;93(3):771–791. [PMC free article] [PubMed] [Google Scholar]
  10. Prince G. A., Porter D. D. The pathogenesis of respiratory syncytial virus infection in infant ferrets. Am J Pathol. 1976 Feb;82(2):339–352. [PMC free article] [PubMed] [Google Scholar]
  11. Richardson L. S., Belshe R. B., London W. T., Sly D. L., Prevar D. A., Camargo E., Chanock R. M. Evaluation of five temperature-sensitive mutants of respiratory syncytial virus in primates: I. Viral shedding, immunologic response, and associated illness. J Med Virol. 1978;3(2):91–100. doi: 10.1002/jmv.1890030202. [DOI] [PubMed] [Google Scholar]
  12. Richardson L. S., Belshe R. B., Sly D. L., London W. T., Prevar D. A., Camargo E., Chanock R. M. Experimental respiratory syncytial virus pneumonia in cebus monkeys. J Med Virol. 1978;2(1):45–59. doi: 10.1002/jmv.1890020108. [DOI] [PubMed] [Google Scholar]
  13. Richardson L. S., Schnitzer T. J., Belshe R. B., Camargo E., Prevar D. A., Chanock R. M. Isolation and characterization of further defective clones of a temperature sensitive mutant (ts-1) of respiratory syncytial virus. Arch Virol. 1977;54(1-2):53–60. doi: 10.1007/BF01314378. [DOI] [PubMed] [Google Scholar]
  14. Schnitzer T. J., Richardson L. S., Chanock R. M. Growth and genetic stability of the ts-1 mutant of respiratory syncytial virus at restrictive temperatures. J Virol. 1976 Feb;17(2):431–438. doi: 10.1128/jvi.17.2.431-438.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Wright P. F., Gharpure M. A., Hodes D. S., Chanock R. M. Genetic studies of respiratory syncytial virus temperature-sensitive mutants. Arch Gesamte Virusforsch. 1973;41(3):238–247. doi: 10.1007/BF01252771. [DOI] [PubMed] [Google Scholar]
  16. Wright P. F., Shinozaki T., Fleet W., Sell S. H., Thompson J., Karzon D. T. Evaluation of a live, attenuated respiratory syncytial virus vaccine in infants. J Pediatr. 1976 Jun;88(6):931–936. doi: 10.1016/s0022-3476(76)81044-x. [DOI] [PubMed] [Google Scholar]
  17. Wright P. F., v Mills J., Chanock R. M. Evaluation of a temperature-sensitive mutant of respiratory syncytial virus in adults. J Infect Dis. 1971 Nov;124(5):505–511. doi: 10.1093/infdis/124.5.505. [DOI] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES