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. 2014 Jun 2;5(12):4071–4086. doi: 10.18632/oncotarget.1705

Figure 7. MLN8237 treatment induces cellular senescence in gastrointestinal stromal tumor (GIST) cell lines.

Figure 7

(A) Representative photomicrographs of GIST48 cells before and after MLN8237 treatment. Upper panel: images of cells after staining for senescence-associated β-galactosidase (SA-β-gal) activity; lower panel: corresponding bright field images. Scale bar = 50 μm. Some SA-β-gal positive cells are indicated by arrows. (B) Treating GIST48 cells with MLN8237 for six days dose-dependently increased SA-β-gal staining. (C) Western blot analysis of phospho-p70 S6 kinase (p-p70S6K) and DEC1 expression relative to that of actin in GIST48 cells treated with MLN8237. (D) Flow cytometric analysis of the expression of DcR2 in GIST48 cells treated with MLN8237 for six days. (E) Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) analysis of interleukin-6 (IL-6) expression relative to that of glyceraldehyde 3-phosphate dehydrogenase (GAPDH) in GIST48 cells treated with MLN8237. *P < 0.05.