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. 2014 May 20;5(12):4257–4268. doi: 10.18632/oncotarget.1991

Figure 2. HDAC2 and N-Myc commonly repress, and BET bromodomain inhibitors up-regulate, TP53INP1 expression.

Figure 2

A and B, Kelly and SK-N-BE(2) neuroblastoma cells were transfected with scrambled control siRNA, N-Myc siRNA-1, N-Myc siRNA-2, HDAC2 siRNA-1 or HDAC2 siRNA-2 for 48 hours. RNA and protein were extracted from the cells and subjected to RT-PCR (A) and immunoblot (B) analyses of TP53INP1 mRNA and protein expression. C and D, Kelly and SK-N-BE(2) cells were treated with vehicle control or various doses of the BET bromodomain inhibitor I-BET151 or JQ1 for 48 hours, followed by RNA and protein extraction. C, N-Myc and TP53INP1 mRNA expression was analysed by RT-PCR. D, TP53INP1 protein expression was analysed by immunoblot. Error bars represent standard error. *, ** and *** indicate P < 0. 05, 0.01 and 0.001, respectively.