The primer Q-PAdV-R was mismatched with probe Q-PAdV-P in Table 1. The table is corrected here.
Table 1.
Oligonucleotide primers and probes used for the detection and quantification of viral indicators.
| Primers and probes | Virus | Positiona | Reference | Sequence (5′-3′) |
|---|---|---|---|---|
| ADF | Human adenovirus (HAdV) | 18869–18887 | [26] | CWTACATGCACATCKCSGG |
| ADR | 18919–18937 | CRCGGGCRAAYTGCACCAG | ||
| ADP1 | 18889–18916 | FAM-CCGGGCTCAGGTACTCCGAGGCGTCCT-BHQ1 | ||
|
| ||||
| JE3F | JC polyomavirus (JCPyV) | 4317–4339 | [27] | ATGTTTGCCAGTGATGATGAAAA |
| JE3R | 4251–4277 | GGAAAGTCTTTAGGGTCTTCTACCTTT | ||
| JE3P | 4313–4482 | FAM-AGGATCCCAACACTCTACCCCACCTAAAAAGA-BHQ1 | ||
|
| ||||
| QB-F1-1 | Bovine polyomavirus (BPyV) | 2122–2144 | [28] | CTAGATCCTACCCTCAAGGGAAT |
| QB-R1-1 | 2177–2198 | TTACTTGGATCTGGACACCAAC | ||
| QB-P1-2 | 2149–2174 | FAM-GACAAAGATGGTGTGTATCCTGTTGA-BHQ1 | ||
|
| ||||
| Q-PAdV-F | Porcine adenovirus (PAdV) | 20701–20718 | [29] | AACGGCCGCTACTGCAAG |
| Q-PAdV-R | 20751–20768 | AGCAGCAGGCTCTTGAGG | ||
| Q-PAdV-P | 20722–20737 | FAM-CACATCCAGGTGCCGC-BHQ1 | ||
|
| ||||
| qOv_F | Ovine polyomavirus (OPyV) | VP1b region | [30] | CAGCTGYAGACATTGTGG |
| qOv_R | TCCAATCTGGGCATAAGATT | |||
| qOv_P | FAM-ATGATTACCAAGCCAGACAGTGGG-BHQ1 | |||
|
| ||||
| Q-PaV-F | Chicken/turkey parvovirus (ChPV/TuPV) | 3326–3345 | [31] | AGTCCACGAGATTGGCAACA |
| Q-PaV-R | 3388–3407 | GCAGGTTAAAGATTTTCACG | ||
| Q-PaV-Pr | 3356–3378 | 6FAM-AATTATTCGAGATGGCGCCCACG-BHQ1 | ||
aThe sequence positions are referred to strains J01917.1 (HAdV), NC_001699.1 (JCPyV), D13942 (BPyV), AJ237815 (PAdV), and GU214706 (ChPV/TuPV) from Genbank. bVP1: virion protein 1.