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. 1974 Jan;9(1):179–186. doi: 10.1128/iai.9.1.179-186.1974

Release of β-Lysin from Platelets by Thrombin and by a Factor Produced in Heparinized Blood

John G Tew 1,2, Robert R Roberts 1,2, David M Donaldson 1,2
PMCID: PMC414782  PMID: 4202888

Abstract

Removal of calcium by either citrate, oxalate, or ethylenediaminetetraacetate inhibited coagulation of blood at an early stage and prevented the release of β-lysin from platelets. In contrast, heparin caused platelet agglutination and stimulated β-lysin release in vitro and in vivo. This release was calcium dependent and may have been due to a calcium-dependent reaction in the blood coagulation sequence. Thrombin which by-passed the early calcium-dependent stages of coagulation directly stimulated the release of β-lysin from platelets. However, thrombin alone or in combination with other plasma factors was not as effective in releasing β-lysin as the regular coagulation process. Thrombin's platelet degranulating activity correlated with its β-lysin releasing activity. In contrast to thrombin, staphylococcal coagulase, which also by-passed the calcium-dependent stages of coagulation, coagulated citrated blood without releasing β-lysin. The release of β-lysin has been observed previously in the absence of blood coagulation, but this is the first observation of coagulation without β-lysin release. It is clear that β-lysin is released from platelets during coagulation by the direct action of thrombin and that it may be released in an earlier calcium-dependent reaction.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bennett B., Ratnoff O. D. The normal coagulation mechanism. Med Clin North Am. 1972 Jan;56(1):95–104. doi: 10.1016/s0025-7125(16)32425-7. [DOI] [PubMed] [Google Scholar]
  2. DONALDSON D. M., ELLSWORTH B., MATHESON A. SEPARATION AND PURIFICATION OF BETA-LYSIN FROM NORMAL SERUM. J Immunol. 1964 Jun;92:896–901. [PubMed] [Google Scholar]
  3. DONALDSON D. M., JENSEN R. S., JENSEN B. M., MATHESON A. SEROLOGICAL RELATIONSHIPS AMONG BETA-LYSIN, PLAKIN, AND LEUKIN. J Bacteriol. 1964 Oct;88:1049–1055. doi: 10.1128/jb.88.4.1049-1055.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. DONALDSON D. M., MARCUS S. Studies on serum bactericidal activity; interrelationships of heparin, citrate, protamine and xirradiation on serum and plasma bactericidal activity against Bacillus subtilis. J Immunol. 1958 Oct;81(4):292–296. [PubMed] [Google Scholar]
  5. Eika C. Heparin-induced platelet release and refractory platelets. Lancet. 1972 Jun 17;1(7764):1343–1344. doi: 10.1016/s0140-6736(72)91077-x. [DOI] [PubMed] [Google Scholar]
  6. Gentry P. W., Alexander B. Specific coagulation factor adsorption to insoluble heparin. Biochem Biophys Res Commun. 1973 Jan 23;50(2):500–509. doi: 10.1016/0006-291x(73)90868-1. [DOI] [PubMed] [Google Scholar]
  7. HIRSCH J. G. Comparative bactericidal activities of blood serum and plasma serum. J Exp Med. 1960 Jul 1;112:15–22. doi: 10.1084/jem.112.1.15. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Hess W. M. Fixation and staining of fungus hyphae and host plant root tissues for electron microscopy. Stain Technol. 1966 Jan;41(1):27–35. doi: 10.3109/10520296609116276. [DOI] [PubMed] [Google Scholar]
  9. MACMILLAN R. L., BROWN K. W. Heparin and thromboplastin. J Lab Clin Med. 1954 Sep;44(3):378–386. [PubMed] [Google Scholar]
  10. Macfarlane R. G. A. The blood clotting mechanism. The development of a theory of blood coagulation. Proc R Soc Lond B Biol Sci. 1969 Jul 1;173(1032):261–268. doi: 10.1098/rspb.1969.0051. [DOI] [PubMed] [Google Scholar]
  11. Mojovic B., Mojovic N., Tager M., Drummond M. C. Staphylocoagulase as a hemostatic agent. Yale J Biol Med. 1969 Aug;42(1):11–20. [PMC free article] [PubMed] [Google Scholar]
  12. Tew J. G., Hess W. M., Donaldson D. M. Lysozyme and beta-lysin release stimulated by antigen-antibody complexes and bacteria. J Immunol. 1969 Mar;102(3):743–750. [PubMed] [Google Scholar]
  13. Tirunarayanan M. O. Investigations on the enzymes and toxins of staphylococci. Stimulation of coagulase biosynthesis by glucose and bicarbonate. Acta Pathol Microbiol Scand. 1966;68(2):273–280. doi: 10.1111/apm.1966.68.2.273. [DOI] [PubMed] [Google Scholar]
  14. ZOLLI Z., Jr, SANCLEMENTE C. L. PURIFICATION AND CHARACTERIZATION OF STAPHYLOCOAGULASE. J Bacteriol. 1963 Sep;86:527–535. doi: 10.1128/jb.86.3.527-535.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]

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