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. 1974 Jan;9(1):187–191. doi: 10.1128/iai.9.1.187-191.1974

Quantitative Evaluation of the Progress of Intracellular Infection In Vitro: Incorporation of 3H-Thymidine into Deoxyribonucleic Acid by Mycobacterium leprae in Cultivated Blood Monocytes

G P Talwar 1, A D Krishnan 1, P D Gupta 1
PMCID: PMC414783  PMID: 4128631

Abstract

Growth of intracellular parasites such as Mycobacterium leprae in macrophages derived from human peripheral blood monocytes can be assessed by selective incorporation of [methyl-3H]thymidine into deoxyribonucleic acid of the bacterial cells. The radioactive precursor is not taken up by the host cells, and evidence has been presented for its incorporation into bacteria. The procedure is sensitive, reproducible, and highly quantitative.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Drutz D. J., Cline M. J. Incorporation of tritiated thymidine by leprosy bacilli in cultures of human lepromatous macrophages. J Infect Dis. 1972 Apr;125(4):416–419. doi: 10.1093/infdis/125.4.416. [DOI] [PubMed] [Google Scholar]
  2. HANKS J. H., CHATTERJEE B. R., LECHAT M. F. A GUIDE TO THE COUNTING OF MYCOBACTERIA IN CLINICAL AND EXPERIMENTAL MATERIALS. Int J Lepr. 1964 Apr-Jun;32:156–167. [PubMed] [Google Scholar]
  3. LUFT J. H. Improvements in epoxy resin embedding methods. J Biophys Biochem Cytol. 1961 Feb;9:409–414. doi: 10.1083/jcb.9.2.409. [DOI] [PMC free article] [PubMed] [Google Scholar]

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