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. 1974 Feb;9(2):229–235. doi: 10.1128/iai.9.2.229-235.1974

Staphylococcal Enterotoxin Synthesis During the Exponential, Transitional, and Stationary Growth Phases

Joyce K Czop 1, Merlin S Bergdoll 1
PMCID: PMC414791  PMID: 4205941

Abstract

Small inocula (1 to 10 colony-forming units per ml of broth) of Staphylococcus aureus strains S-6, S-6R, and FRI-100 were employed to study growth and enterotoxin synthesis in 4% protein hydrolysate powder broths. For each strain, the exponential growth phase ended once the population approached 109 to 2 × 109 colony-forming units per ml. By that time, the concentrations of enterotoxins A and B reached the minimal level (1 to 2 μg/ml) at which the single gel diffusion tube method becomes applicable. By microslides and reverse passive hemagglutination, enterotoxins A and B were found to be synthesized during the exponential growth phase, but at different exponential rates.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BARBER M., KUPER S. W. A. Identification of Staphylococcus pyogenes by the phosphatase reaction. J Pathol Bacteriol. 1951 Jan;63(1):65–68. doi: 10.1002/path.1700630108. [DOI] [PubMed] [Google Scholar]
  2. CASMAN E. P., BENNETT R. W. DETECTION OF STAPHYLOCOCCAL ENTEROTOXIN IN FOOD. Appl Microbiol. 1965 Mar;13:181–189. doi: 10.1128/am.13.2.181-189.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Dietrich G. G., Watson R. J., Silverman G. J. Effect of shaking speed on the secretion of enterotoxin B by Staphylococcus aureus. Appl Microbiol. 1972 Oct;24(4):561–566. doi: 10.1128/am.24.4.561-566.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Genigeorgis C., Sadler W. W. Effect of sodium chloride and pH on enterotoxin B production. J Bacteriol. 1966 Nov;92(5):1383–1387. doi: 10.1128/jb.92.5.1383-1387.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. HUNT G. A., GOUREVITCH A., LEIN J. Preservation of cultures by drying on porcelain beads. J Bacteriol. 1958 Oct;76(4):453–454. doi: 10.1128/jb.76.4.453-454.1958. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Kato E., Khan M., Kujovich L., Bergdoll M. S. Production of enterotoxin a. Appl Microbiol. 1966 Nov;14(6):966–972. doi: 10.1128/am.14.6.966-972.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  8. Markus Z. H., Silverman G. J. Factors affecting the secretion of staphylococcal enterotoxin A. Appl Microbiol. 1970 Sep;20(3):492–496. doi: 10.1128/am.20.3.492-496.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Markus Z., Silverman G. J. Enterotoxin B synthesis by replicating and nonreplicating cells of Staphylococcus aureus. J Bacteriol. 1969 Feb;97(2):506–512. doi: 10.1128/jb.97.2.506-512.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. McLean R. A., Lilly H. D., Alford J. A. Effects of meat-curing salts and temperature on production of staphylococcal enterotoxin B. J Bacteriol. 1968 Apr;95(4):1207–1211. doi: 10.1128/jb.95.4.1207-1211.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Morse S. A., Baldwin J. N. Regulation of staphylococcal enterotoxin B: effect of thiamine starvation. Appl Microbiol. 1971 Aug;22(2):242–249. doi: 10.1128/am.22.2.242-249.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Reiser R. F., Weiss K. F. Production of staphylococcal enterotoxins A, B, and C in various media. Appl Microbiol. 1969 Dec;18(6):1041–1043. doi: 10.1128/am.18.6.1041-1043.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. SCHAECHTER M., MAALOE O., KJELDGAARD N. O. Dependency on medium and temperature of cell size and chemical composition during balanced grown of Salmonella typhimurium. J Gen Microbiol. 1958 Dec;19(3):592–606. doi: 10.1099/00221287-19-3-592. [DOI] [PubMed] [Google Scholar]
  14. Silverman S. J., Knott A. R., Howard M. Rapid, sensitive assay for staphylococcal enterotoxin and a comparison of serological methods. Appl Microbiol. 1968 Jul;16(7):1019–1023. doi: 10.21236/ad0838753. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Troller J. A. Effect of water activity on enterotoxin B production and growth of Staphylococcus aureus. Appl Microbiol. 1971 Mar;21(3):435–439. doi: 10.1128/am.21.3.435-439.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Weiss K. F., Robbins R. N. Relationship between staphylococcal antiserum titer and zone development on immune serum plates. Appl Microbiol. 1970 Jun;19(6):911–914. doi: 10.1128/am.19.6.911-914.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Wu C. H., Bergdoll M. S. Stimulation of Enterotoxin B Production II. Synthetic Medium for Staphylococcal Growth and Enterotoxin B Production. Infect Immun. 1971 Jun;3(6):784–792. doi: 10.1128/iai.3.6.784-792.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Wu C. H., Bergdoll M. S. Stimulation of enterotoxin B production I. Stimulation by fractions from a pancreatic digest of casein. Infect Immun. 1971 Jun;3(6):777–783. doi: 10.1128/iai.3.6.777-783.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]

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