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. 1974 Feb;9(2):348–353. doi: 10.1128/iai.9.2.348-353.1974

Vi Antigen from Salmonella typhosa and Immunity Against Typhoid Fever I. Isolation and Immunologic Properties in Animals

K H Wong 1,2, John C Feeley 1,2, R S Northrup 1,2,1, M E Forlines 1,2
PMCID: PMC414808  PMID: 4205947

Abstract

The role of Vi antigen in human immunity against typhoid fever has been debated for decades. Circumstantial evidence indicates that Vi antigen may play a role in pathogenicity and immunity. A Vi preparation was isolated from Salmonella typhosa, the causative organism in human typhoid fever, by a mild precipitation method. It was significantly more potent in animal studies than preparations from Citrobacter used in the past for human study and less toxic than conventional typhoid vaccines. With this antigen, the role of Vi antigen in human protection is now being investigated.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BAKER E. E., WHITESIDE R. E., BASCH R., DEROW M. A. The VI antigens of the Enterobacteriaceae. I. Purification and chemical properties. J Immunol. 1959 Dec;83:680–696. [PubMed] [Google Scholar]
  2. CVJETANOVIC B., UEMURA K. THE PRESENT STATUS OF FIELD AND LABORATORY STUDIES OF TYPHOID AND PARATYPHOID VACCINES WITH SPECIAL REFERENCE TO STUDIES SPONSORED BY WORLD HEALTH ORGANIZATION. Bull World Health Organ. 1965;32:29–36. [PMC free article] [PubMed] [Google Scholar]
  3. Goldschneider I., Gotschlich E. C., Artenstein M. S. Human immunity to the meningococcus. I. The role of humoral antibodies. J Exp Med. 1969 Jun 1;129(6):1307–1326. doi: 10.1084/jem.129.6.1307. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Hornick R. B., Greisman S. E., Woodward T. E., DuPont H. L., Dawkins A. T., Snyder M. J. Typhoid fever: pathogenesis and immunologic control. N Engl J Med. 1970 Sep 24;283(13):686–691. doi: 10.1056/NEJM197009242831306. [DOI] [PubMed] [Google Scholar]
  5. Jones G. W., Rutter J. M. Role of the K88 antigen in the pathogenesis of neonatal diarrhea caused by Escherichia coli in piglets. Infect Immun. 1972 Dec;6(6):918–927. doi: 10.1128/iai.6.6.918-927.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. LANDY M. Enhancement of the immunogenicity of typhoid vaccine by retention of the V1 antigen. Am J Hyg. 1953 Sep;58(2):148–164. doi: 10.1093/oxfordjournals.aje.a119596. [DOI] [PubMed] [Google Scholar]
  7. LANDY M., LAMB E. Estimation of Vi antibody employing erythrocytes treated with purified Vi antigen. Proc Soc Exp Biol Med. 1953 Apr;82(4):593–598. doi: 10.3181/00379727-82-20188. [DOI] [PubMed] [Google Scholar]
  8. Pittman M., Bohner H. J. Laboratory assays of different types of field trial typhoid vaccines and relationship to efficacy in man. J Bacteriol. 1966 May;91(5):1713–1723. doi: 10.1128/jb.91.5.1713-1723.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. TULLY J. G., GAINES S. H antigen of Salmonella typhosa. J Bacteriol. 1961 Jun;81:924–932. doi: 10.1128/jb.81.6.924-932.1961. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. TULLY J. G., GAINES S., TIGER TT W. D. Studies on infection and immunity in experimental typhoid fever. IV. Role of H antigen in protection. J Infect Dis. 1963 Mar-Apr;112:118–124. doi: 10.1093/infdis/112.2.118. [DOI] [PubMed] [Google Scholar]
  11. Venneman M. R., Berry L. J. Cell-mediated resistance induced with immunogenic preparations of Salmonella typhimurium. Infect Immun. 1971 Oct;4(4):381–387. doi: 10.1128/iai.4.4.381-387.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. WEBSTER M. E., LANDY M., FREEMAN M. E. Studies on Vi antigen. II. Purification of Vi antigen from Escherichia coli 5395/38. J Immunol. 1952 Aug;69(2):135–142. [PubMed] [Google Scholar]
  13. Williams R. C., Gibbons R. J. Inhibition of bacterial adherence by secretory immunoglobulin A: a mechanism of antigen disposal. Science. 1972 Aug 25;177(4050):697–699. doi: 10.1126/science.177.4050.697. [DOI] [PubMed] [Google Scholar]
  14. Wong K. H., Feeley J. C. Isolation of Vi antigen and a simple method for its measurement. Appl Microbiol. 1972 Oct;24(4):628–633. doi: 10.1128/am.24.4.628-633.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Wong K. H., Feeley J. C., Pittman M. Effect of a Vi-degrading enzyme on potency of typhoid vaccines in mice. J Infect Dis. 1972 Apr;125(4):360–366. doi: 10.1093/infdis/125.4.360. [DOI] [PubMed] [Google Scholar]
  16. Wong K. H., Miller C. E., Feeley J. C., Chan Y. K. Determination of endotoxicity in bacterial vaccines. Appl Microbiol. 1973 Mar;25(3):403–407. doi: 10.1128/am.25.3.403-407.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Wong K. H., Pickett M. J., Froman S. Antigens of mycobacterial cell walls. Infect Immun. 1970 Apr;1(4):400–407. doi: 10.1128/iai.1.4.400-407.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]

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