Skip to main content
NCBI home page
Infection and Immunity logoLink to Infection and Immunity
. 1974 Feb;9(2):444–451. doi: 10.1128/iai.9.2.444-451.1974

Biological and Physical Properties of the Suckling Mouse Cataract Agent Grown in Chicken Embryos

Jesus W Bastardo a,1, David Ou a, Robert H Bussell a
PMCID: PMC414822  PMID: 4205951

Abstract

Experiments were carried out with the suckling mouse cataract agent (SMCA) in an attempt to better characterize the nature of this agent. Despite the fact that high titers of SMCA were found in the chorioallantoic fluid of inoculated chicken embryos, the agent did not replicate in chicken embryo epithelial cell cultures. SMCA did not interfere with Sindbis virus plaque formation and was not inhibited by chicken interferon or polyinosinic-polycytidylic acid. The agent was relatively resistant to high pH and osmotic shock but was inactivated by low pH. Electron micrographs of negatively stained particles concentrated and semipurified from infected chicken embryo chorioallantoic fluids revealed pleomorphic particles ranging from approximately 70 to 400 nm in diameter. SMCA was resistant to kanamycin, novobiocin, and sodium aurothiomalate.

Full text

PDF
444

Images in this article

449Image
on p.449

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ARMSTRONG D., HENLE G., SOMERSON N. L., HAYFLICK L. CYTOPATHOGENIC MYCOPLASMAS ASSOCIATED WITH TWO HUMAN TUMORS. I. ISOLATION AND BIOLOGICAL ASPECTS. J Bacteriol. 1965 Aug;90:418–424. doi: 10.1128/jb.90.2.418-424.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Armstrong D., Paucker K. Effect of mycoplasma on interferon production and interferon assay in cell cultures. J Bacteriol. 1966 Jul;92(1):97–101. doi: 10.1128/jb.92.1.97-101.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Balduzzi P., Charbonneau R. J. Decontamination of pleuropneumonia-like organism (PPLO) infected tissue cultures. Experientia. 1964 Nov 15;20(11):651–652. doi: 10.1007/BF02144845. [DOI] [PubMed] [Google Scholar]
  4. Bastardo J. W., Bergold G. H., Munz K. Structure of Oriboca, Restan, and Manzanilla arboviruses. Am J Trop Med Hyg. 1968 Jan;17(1):115–119. doi: 10.4269/ajtmh.1968.17.115. [DOI] [PubMed] [Google Scholar]
  5. Buskirk H. H. Control of Pleuropneumonia-like Organisms in Cell Culture. Appl Microbiol. 1967 Nov;15(6):1442–1446. doi: 10.1128/am.15.6.1442-1446.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. CLARK H. F. SUCKLING MOUSE CATARACT AGENT. J Infect Dis. 1964 Dec;114:476–487. doi: 10.1093/infdis/114.5.476. [DOI] [PubMed] [Google Scholar]
  7. Clark H. F., Karson D. T. Suckling mouse cataract agent (SMCA) in mice. I. Factors affecting the incidence of cataracts and growth of virus in several strains of mice. J Immunol. 1968 Oct;101(4):776–781. [PubMed] [Google Scholar]
  8. Clark H. F., Karzon D. T. Demonstration of neutralizing antibody to the suckling mouse cataract agent (SMCA). Proc Soc Exp Biol Med. 1969 Apr;130(4):1134–1139. doi: 10.3181/00379727-130-33736. [DOI] [PubMed] [Google Scholar]
  9. Clark H. F., Karzon D. T. Growth curve studies of the suckling mouse cataract agent in individual compartments of the eye. Proc Soc Exp Biol Med. 1969 Jul;131(3):693–696. doi: 10.3181/00379727-131-33954. [DOI] [PubMed] [Google Scholar]
  10. Clark H. F., Karzon D. T. Suckling mouse cataract agent (SMCA) in mice. 3. The antigenic specificity of maternal protection against SMCA, and the comparative ability of other viruses to replicate in the eye of the suckling mouse. J Immunol. 1968 Nov;101(5):890–895. [PubMed] [Google Scholar]
  11. Clark H. F., Karzon D. T. Suckling mouse cataract agent (SMCA) in mice. II. Transfer of protection of progeny of SMCA-infected dams. J Immunol. 1968 Oct;101(4):782–787. [PubMed] [Google Scholar]
  12. Clark H. F. Rat cataract induced by suckling mouse cataract agent. Am J Ophthalmol. 1969 Aug;68(2):304–308. doi: 10.1016/0002-9394(69)94074-4. [DOI] [PubMed] [Google Scholar]
  13. Cross G. F., Goodman M. R., Chatterji J., Beswick T. S., Chapman J. A. Another case of mistaken identity: rubella and mycoplasma. J Gen Virol. 1970 Jul;8(1):77–81. doi: 10.1099/0022-1317-8-1-77. [DOI] [PubMed] [Google Scholar]
  14. Cross G. F., Goodman M. R., Shaw E. J. Detection and treatment of contaminating mycoplasmas in cell culture. Aust J Exp Biol Med Sci. 1967 Apr;45(2):201–212. doi: 10.1038/icb.1967.15. [DOI] [PubMed] [Google Scholar]
  15. Elizan T. S., Fabiyi A., Clark H. F. Suckling mouse cataract agent (SMCA)-induced hydrocephalus and chronic brain infection in newborn rats. Proc Soc Exp Biol Med. 1972 Jan;139(1):51–55. doi: 10.3181/00379727-139-36074. [DOI] [PubMed] [Google Scholar]
  16. Fabiyi A., Elizan T. S., Pounds J. E. Suckling mouse cataract agent (SMCA) in tissue culture. Proc Soc Exp Biol Med. 1971 Jan;136(1):88–91. doi: 10.3181/00379727-136-35200. [DOI] [PubMed] [Google Scholar]
  17. Field A. K., Tytell A. A., Lampson G. P., Hilleman M. R. Inducers of interferon and host resistance. II. Multistranded synthetic polynucleotide complexes. Proc Natl Acad Sci U S A. 1967 Sep;58(3):1004–1010. doi: 10.1073/pnas.58.3.1004. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. NEWNHAM A. G., CHU H. P. AN IN VITRO COMPARISON OF THE EFFECT OF SOME ANTIBACTERIAL, ANTIFUNGAL AND ANTIPROTOZOAL AGENTS ON VARIOUS STRAINS OF MYCOPLASMA (PLEUROPNEUMONIA-LIKE ORGANISMS: P.P.L.O.). J Hyg (Lond) 1965 Mar;63:1–23. doi: 10.1017/s0022172400044922. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Perlman D., Rahman S. B., Semar J. B. Antibiotic control of Mycoplasma in tissue culture. Appl Microbiol. 1967 Jan;15(1):82–85. doi: 10.1128/am.15.1.82-85.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. RAZIN S., ARGAMAN M. Lysis of Mycoplasma, bacterial protoplasts, spheroplasts and L-forms by various agents. J Gen Microbiol. 1963 Jan;30:155–172. doi: 10.1099/00221287-30-1-155. [DOI] [PubMed] [Google Scholar]
  21. RAZIN S. FACTORS INFLUENCING OSMOTIC FRAGILITY OF MYCOPLASMA. J Gen Microbiol. 1964 Sep;36:451–459. doi: 10.1099/00221287-36-3-451. [DOI] [PubMed] [Google Scholar]
  22. WAGNER R. R. Biological studies of interferon. I. Suppression of cellular infection with eastern equine encephalomyelitis virus. Virology. 1961 Mar;13:323–337. doi: 10.1016/0042-6822(61)90152-0. [DOI] [PubMed] [Google Scholar]
  23. Yershov F. I., Zhdanov V. M. Influence of PPLO on production of interferon in virus-infected cells. Virology. 1965 Nov;27(3):451–453. doi: 10.1016/0042-6822(65)90132-7. [DOI] [PubMed] [Google Scholar]
  24. Zeigel R. F., Clark H. F. Electron microscopy of the suckling mouse cataract agent: a noncultivable animal pathogen possibly related to mycoplasma. Infect Immun. 1974 Feb;9(2):430–443. doi: 10.1128/iai.9.2.430-443.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES