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a) Instrument setup for MS/MS with CID during mass selective ion transfer. MS/MS spectra recorded for mass-selective transfer of protonated amitriptyline m/z 278 at RIT DC offset at b) −15V, c) −21V and d) −40V. AC excitation for axial ejection from QLIT, 80 mV and 169 kHz, q = 0.45 in QLIT; q = 0.2 for trapping the ions in RIT.
