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. 1974 Mar;9(3):524–529. doi: 10.1128/iai.9.3.524-529.1974

Corticosteroids, Serum, and Phagocytosis: In Vitro and In Vivo Studies

John W Olds a,1, William P Reed a, Betty Eberle a, Alexander L Kisch a
PMCID: PMC414838  PMID: 4816631

Abstract

The phagocytic-bactericidal capacity (PBC) of human polymorphonuclear leukocytes (PMNs) for strains of Klebsiella (K) and of Enterococcus (E) was unaffected in vitro by the presence of 100 μg of either hydrocortisone (HC) or of methylprednisolone (MP) per ml in the medium. At higher concentrations (500 to 2,000 μg/ml) both compounds impaired PBC-K and PBC-E, but the latter was less sensitive to steroid-induced inhibition. In addition to interfering with intracellular killing of both organisms by PMNs, 2,000 μg of HC per ml also inhibited ingestion of E, although not of K. Steroid-induced inhibition of PBC-K in vitro was completely abolished by increasing the concentration of serum used as opsonin. The PBC-K of human PMNs obtained 30 min after intravenous injection of 1 g of MP was unimpaired in vitro in the presence of 10 to 90% simultaneously obtained autologous serum containing 42 μg of MP per ml. These findings suggest that short-term, high-dosage administration of MP is unlikely to produce clinically significant impairment of intraleukocytic bacterial killing.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ALLISON F., Jr, ADCOCK M. H. FAILURE OF PRETREATMENT WITH GLUCOCORTICOIDS TO MODIFY THE PHAGOCYTIC AND BACTERICIDAL CAPACITY OF HUMAN LEUKOCYTES FOR ENCAPSULATED TYPE I PNEUMOCOCCUS. J Bacteriol. 1965 May;89:1256–1261. doi: 10.1128/jb.89.5.1256-1261.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. CREPEA S. B., MAGNIN G. E., SEASTONE C. V. Effect of ACTH and cortisone on phagocytosis. Proc Soc Exp Biol Med. 1951 Aug;77(4):704–706. doi: 10.3181/00379727-77-18899. [DOI] [PubMed] [Google Scholar]
  3. Christy J. H. Treatment of gram-negative shock. Am J Med. 1971 Jan;50(1):77–88. doi: 10.1016/0002-9343(71)90207-5. [DOI] [PubMed] [Google Scholar]
  4. HIRSCH J. G., STRAUSS B. STUDIES ON HEAT-LABILE OPSONIN IN RABBIT SERUM. J Immunol. 1964 Jan;92:145–154. [PubMed] [Google Scholar]
  5. KASS E. H., FINLAND M. Adrenocortical hormones and the management of infection. Annu Rev Med. 1957;8:1–18. doi: 10.1146/annurev.me.08.020157.000245. [DOI] [PubMed] [Google Scholar]
  6. Klastersky J., Cappel R., Debusscher L. Effectiveness of betamethasone in management of severe infections. A double-blind study. N Engl J Med. 1971 Jun 3;284(22):1248–1250. doi: 10.1056/NEJM197106032842206. [DOI] [PubMed] [Google Scholar]
  7. LIDDLE G. W. Studies of structure-function relationships of steroids. II. The 6 alpha-methylcorticosteroids. Metabolism. 1958 Jul;7(4 Pt 2):405–415. [PubMed] [Google Scholar]
  8. MILLS I. H. Transport and metabolism of steroids. Br Med Bull. 1962 May;18:127–133. doi: 10.1093/oxfordjournals.bmb.a069953. [DOI] [PubMed] [Google Scholar]
  9. Mandell G. L., Hook E. W. Leukocyte bactericidal activity in chronic granulomatous disease: correlation of bacterial hydrogen peroxide production and susceptibility to intracellular killing. J Bacteriol. 1969 Oct;100(1):531–532. doi: 10.1128/jb.100.1.531-532.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Mandell G. L., Rubin W., Hook E. W. The effect of an NADH oxidase inhibitor (hydrocortisone) on polymorphonuclear leukocyte bactericidal activity. J Clin Invest. 1970 Jul;49(7):1381–1388. doi: 10.1172/JCI106355. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Olds J. W., Kisch A. L., Eberle B. J., Wilson J. N. Pseudomonas aeruginosa respiratory tract infection acquired from a contaminated anesthesia machine. Am Rev Respir Dis. 1972 Apr;105(4):628–632. doi: 10.1164/arrd.1972.105.4.628. [DOI] [PubMed] [Google Scholar]
  12. PETERSON R. E., WYNGAARDEN J. B., GUERRA S. L., BRODIE B. B., BUNIM J. J. The physiological disposition and metabolic fate of hydrocortisone in man. J Clin Invest. 1955 Dec;34(12):1779–1794. doi: 10.1172/JCI103233. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Seneca H., Peer P. Effect of antibacterials, antibiotics, enzymes and steroids on phagocytosis. J Am Geriatr Soc. 1966 Mar;14(3):187–199. doi: 10.1111/j.1532-5415.1966.tb05308.x. [DOI] [PubMed] [Google Scholar]

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