Abstract
Anti-phosphatidylglycerol and anti-cardiolipin antisera were prepared in rabbits by using phospholipids purified from Micrococcus lysodeikticus. Anti-phosphatidylglycerol antibodies were found in antisera when either phosphatidylglycerol or cardiolipin were used as immunogens, but adsorption studies indicated they were not similar. Antibodies which reacted with phosphatidylinositol and phosphatidic acid were also found in the anti-cardiolipin antiserum. Structures of the antigenic groups in phosphatidylglycerol and cardiolipin are suggested from cross-reaction and adsorption studies. Adsorption studies with pure phospholipid antigens indicated the importance of the spacial orientation of phospholipid haptens for immunological reactivity.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- De Siervo A. J., Salton M. R. Biosynthesis of cardiolipin in the membranes of Micrococcus lysodeikticus. Biochim Biophys Acta. 1971 Jul 13;239(2):280–292. doi: 10.1016/0005-2760(71)90174-3. [DOI] [PubMed] [Google Scholar]
- FOWLER E., ALLEN R. H. Studies on the production of Wassermann reagin. J Immunol. 1962 May;88:591–594. [PubMed] [Google Scholar]
- Guarnieri M., Stechmiller B., Lehninger A. L. Use of an antibody to study the location of cardiolipin in mitochondrial membranes. J Biol Chem. 1971 Dec 25;246(24):7526–7532. [PubMed] [Google Scholar]
- Inoue K., Nojima S. Immunochemical studies of phospholipids. 3. Production of antibody to cardiolipin. Biochim Biophys Acta. 1967 Oct 2;144(2):409–414. [PubMed] [Google Scholar]
- Kataoka T., Nojima S. Immunochemical studies of phospholipids. VI. Haptenic activity of phosphatidylinositol and the role of lecithin as an auxiliary lipid. J Immunol. 1970 Aug;105(2):502–511. [PubMed] [Google Scholar]
- Whiteside T. L., De Siervo A. J., Salton M. R. Use of antibody to membrane adenosine triphosphatase in the study of bacterial relatioships. J Bacteriol. 1971 Mar;105(3):957–967. doi: 10.1128/jb.105.3.957-967.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]