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. 1974 May;9(5):858–862. doi: 10.1128/iai.9.5.858-862.1974

Respiratory Tract Cell-Mediated Immunity: Comparison of Primary and Secondary Response

N Gadol 1, J E Johnson III 1, R H Waldman 1
PMCID: PMC414898  PMID: 4824634

Abstract

A secondary local and splenic cell-mediated immune response was observed and compared to the primary response. Previous studies have demonstrated cell-mediated immunity (CMI) by lymphocytes from bronchopulmonary washings and have shown that its appearance is to a large extent inedpendent of splenic CMI. This study evaluated the secondary as compared to the primary response, with respect to both cellular and humoral immune responses. Guinea pigs were immunized with influenza virus vaccine either nasally or parenterally, booster immunizations were given by the same route, and animals were killed at various times after immunization or booster. The inhibition of macrophage migration was used to assess CMI. As in previous studies, local application of antigen led to mainly local appearance of CMI, whereas parenteral immunization led to mainly systemic CMI. Both pulmonary and splenic lymphocytes showed an inhibition of macrophage migration that appeared 2 to 3 days sooner after the booster, as compared to the primary immunization. There was no evidence, however, for the earlier production or increased amount of antibody in the bronchial secretions in the boosted animals. The results suggest that pulmonary as well as splenic T lymphocytes exhibit memory, but that pulmonary B lymphocytes do not.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Buscho R. F., Perkins J. C., Knopf H. L., Kapikian A. Z., Chanock R. M. Further characterization of the local respiratory tract antibody response induced by intranasal instillation of inactivated rhinovirus 13 vaccine. J Immunol. 1972 Jan;108(1):169–177. [PubMed] [Google Scholar]
  2. CHANOCK R. M., PARROTT R. H., COOK K., ANDREWS B. E., BELL J. A., REICHELDERFER T., KAPIKIAN A. Z., MASTROTA F. M., HUEBNER R. J. Newly recognized myxoviruses from children with respiratory disease. N Engl J Med. 1958 Jan 30;258(5):207–213. doi: 10.1056/NEJM195801302580502. [DOI] [PubMed] [Google Scholar]
  3. David J. R. Suppression of delayed hypersensitivity in vitro by inhibition of protein synthesis. J Exp Med. 1965 Dec 1;122(6):1125–1134. doi: 10.1084/jem.122.6.1125. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Galindo B., Myrvik Q. N. Migratory response of granulomatous alveolar cells from BCG-sensitized rabbits. J Immunol. 1970 Jul;105(1):227–237. [PubMed] [Google Scholar]
  5. Henney C. S., Waldman R. H. Cell-mediated immunity shown by lymphocytes from the respiratory tract. Science. 1970 Aug 14;169(3946):696–697. doi: 10.1126/science.169.3946.696. [DOI] [PubMed] [Google Scholar]
  6. KAWATA H., MYRVIK Q. N., LEAKE E. S. DISSOCIATION OF TUBERCULIN HYPERSENSITIVITY AS MEDIATOR FOR AN ACCELERATED PULMONARY GRANULOMATOUS RESPONSE IN RABBITS. J Immunol. 1964 Sep;93:433–438. [PubMed] [Google Scholar]
  7. Nash D. R., Holle B. Local and systemic cellular immune responses in guinea-pigs given antigen parenterally or directly into the lower respiratory tract. Clin Exp Immunol. 1973 Apr;13(4):573–583. [PMC free article] [PubMed] [Google Scholar]
  8. Ogra P. L., Karzon D. T. Poliovirus antibody response in serum and nasal secretions following intranasal inoculation with inactivated poliovaccine. J Immunol. 1969 Jan;102(1):15–23. [PubMed] [Google Scholar]
  9. Raff M. C. Role of thymus-derived lymphocytes in the secondary humoral immune response in mice. Nature. 1970 Jun 27;226(5252):1257–1258. doi: 10.1038/2261257a0. [DOI] [PubMed] [Google Scholar]
  10. Smith J. B., Cunningham A. J., Lafferty K. J., Morris B. The role of the lymphatic system and lymphoid cells in the establishment of immunological memory. Aust J Exp Biol Med Sci. 1970 Feb;48(1):57–70. doi: 10.1038/icb.1970.6. [DOI] [PubMed] [Google Scholar]
  11. Takahashi T., Carswell E. A., Thorbecke G. J. Surface antigens of immunocompetent cells. I. Effect of theta and PC.1 alloantisera on the ability of spleen cells to transfer immune responses. J Exp Med. 1970 Dec 1;132(6):1181–1190. doi: 10.1084/jem.132.6.1181. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Waldman R. H., Henney C. S. Cell-mediated immunity and antibody responses in the respiratory tract after local and systemic immunization. J Exp Med. 1971 Aug 1;134(2):482–494. doi: 10.1084/jem.134.2.482. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Waldman R. H., Spencer C. S., Johnson J. E., 3rd Respiratory and systemic cellular and humoral immune responses to influenza virus vaccine administered parenterally or by nose drops. Cell Immunol. 1972 Feb;3(2):294–300. doi: 10.1016/0008-8749(72)90168-2. [DOI] [PubMed] [Google Scholar]

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