Abstract
Complement-fixing activity of a T strain of Mycoplasma was found to be associated with its lipid components. Heat stability and lability of periodate and β-glucosidase treatments led to the conclusion that complement-fixing, active lipids had carboydrate determinants. Periodate treatment of chromatographic fractions of a chloroform-methanol extract showed that only antigens contained in the acetone fraction were periodate labile. Lipids also appeared to be involved in the passive hemagglutination, since the lipid fraction active in complement fixation also combined and blocked the action of antibodies which agglutinated sensitized erythrocytes with strain P108.
Full text
PDF



Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- ENNY G. E., GRAYSTON J. T. EATON PLEUROPNEUMONIA-LIKE ORGANISM (MYCOPLASMA PNEUMONIAE) COMPLEMENT-FIXING ANTIGEN: EXTRACTION WITH ORGANIC SOLVENTS. J Immunol. 1965 Jul;95:19–25. [PubMed] [Google Scholar]
- Kenny G. E. Serological cross-reaction between lipids of Mycoplasma pneumoniae and Mycoplasma neurolyticum. Infect Immun. 1971 Aug;4(2):149–153. doi: 10.1128/iai.4.2.149-153.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
- LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
- Lemcke R. M., Marmion B. P., Plackett P. Immunochemical analysis of Mycoplasma pneumoniae. Ann N Y Acad Sci. 1967 Jul 28;143(1):691–702. doi: 10.1111/j.1749-6632.1967.tb27715.x. [DOI] [PubMed] [Google Scholar]
- Plackett P., Marmion B. P., Shaw E. J., Lemcke R. M. Immunochemical analysis of Mycoplasma pneumoniae. 3. Separation and chemical identification of serologically active lipids. Aust J Exp Biol Med Sci. 1969 Apr;47(2):171–195. doi: 10.1038/icb.1969.19. [DOI] [PubMed] [Google Scholar]
- RAPPORT M. M., GRAF L., SKIPSKI V. P., ALONZO N. F. Immunochemical studies of organ and tumor lipids. VI. Isolation and properties of cytolipin H. Cancer. 1959 May-Jun;12(3):438–445. doi: 10.1002/1097-0142(195905/06)12:3<438::aid-cncr2820120303>3.0.co;2-o. [DOI] [PubMed] [Google Scholar]
- Romano N., Smith P. F., Mayberry W. R. Lipids of a T strain of Mycoplasma. J Bacteriol. 1972 Feb;109(2):565–569. doi: 10.1128/jb.109.2.565-569.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
- SEVER J. L. Application of a microtechnique to viral serological investigations. J Immunol. 1962 Mar;88:320–329. [PubMed] [Google Scholar]
- Smith P. F. Lipid composition of Mycoplasma neurolyticum. J Bacteriol. 1972 Oct;112(1):554–558. doi: 10.1128/jb.112.1.554-558.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sobeslavsky O., Prescott B., James W. D., Chanock R. M. Isolation and characterization of fractions of Mycoplasma pneumoniae. II. Antigenicity and immunogenicity. J Bacteriol. 1966 Jun;91(6):2126–2138. doi: 10.1128/jb.91.6.2126-2138.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
- YOKOYAMA M., TRAMS E. G., BRADY R. O. Sphingolipid antibodies in sera of animals and patients with central nervous system lesions. Proc Soc Exp Biol Med. 1962 Nov;111:350–352. doi: 10.3181/00379727-111-27789. [DOI] [PubMed] [Google Scholar]