Skip to main content
NCBI home page
Infection and Immunity logoLink to Infection and Immunity
. 1974 Jul;10(1):11–15. doi: 10.1128/iai.10.1.11-15.1974

Persistence of Adenovirus 5 in Guinea Pigs

N Faucon 1, Y Chardonnet 1, R Sohier 1
PMCID: PMC414948  PMID: 4366914

Abstract

One intracardiac inoculation of adenovirus 5 in guinea pigs leads to virus persistence in different organs, viz., 5 days in lungs and liver, 14 days in blood and lymph nodes, and 56 days or more in the spleen. After cultivation of tissue cells for 1 week, virus was recovered from blood, lymph nodes, or spleen lymphocytes, but virus could be detected directly in cells only when organs were removed within 48 h of inoculation. To determine how the virus persisted in low concentrations and as a latent infection, spleens were primarily selected for study by three techniques: homogenization of spleens, suspended Maitland fragment cultures, and in vitro cultivation of spleen cells. The last procedure showed virus in fibroblast-like cells (probably macrophages or reticuloendothelial cells) for 56 days after infection of guinea pigs. With other methods, the virus was found only within the first 2 days after inoculation.

Full text

PDF
11

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. EVANS A. S. Latent adenovirus infections of the human respiratory tract. Am J Hyg. 1958 May;67(3):256–266. doi: 10.1093/oxfordjournals.aje.a119932. [DOI] [PubMed] [Google Scholar]
  2. HEGGIE A. D., MORGAN H. R. Latent viral infection of cells in tissue culture. III. Role of certain amino acids. Proc Soc Exp Biol Med. 1956 Jul;92(3):506–509. doi: 10.3181/00379727-92-22526. [DOI] [PubMed] [Google Scholar]
  3. HUEBNER R. J., ROWE W. P., WARD T. G., PARROTT R. H., BELL J. A. Adenoidal-pharyngeal-conjunctival agents: a newly recognized group of common viruses of the respiratory system. N Engl J Med. 1954 Dec 30;251(27):1077–1086. doi: 10.1056/NEJM195412302512701. [DOI] [PubMed] [Google Scholar]
  4. Hellmann W., Kohlhage H. Role of spleen in production of virus induced interferon in rabbits. Brief report. Arch Gesamte Virusforsch. 1972;39(4):396–400. doi: 10.1007/BF01241021. [DOI] [PubMed] [Google Scholar]
  5. KAPLAN A. S. The susceptibility of monkey kidney cells to poliovirus in vivo and in vitro. Virology. 1955 Nov;1(4):377–392. doi: 10.1016/0042-6822(55)90031-3. [DOI] [PubMed] [Google Scholar]
  6. Lawrence W. C., Ginsberg H. S. Intracellular uncoating of type 5 adenovirus deoxyribonucleic acid. J Virol. 1967 Oct;1(5):851–867. doi: 10.1128/jvi.1.5.851-867.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. MIMS C. A. ASPECTS OF THE PATHOGENESIS OF VIRUS DISEASES. Bacteriol Rev. 1964 Mar;28:30–71. doi: 10.1128/br.28.1.30-71.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. MORGAN H. R. Latent viral infection of cells in tissue culture. I. Studies on latent infection of chick embryo tissues with psittacosis virus. J Exp Med. 1956 Jan 1;103(1):37–47. doi: 10.1084/jem.103.1.37. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Nász I., Kulcsár G., Dán P., Sallay K. A possible pathogenic role for virus-carrier lymphocytes. J Infect Dis. 1971 Aug;124(2):214–216. doi: 10.1093/infdis/124.2.214. [DOI] [PubMed] [Google Scholar]
  10. PEREIRA H. G., KELLY B. Latent infection of rabbits by adenovirus type 5. Nature. 1957 Sep 21;180(4586):615–616. doi: 10.1038/180615b0. [DOI] [PubMed] [Google Scholar]
  11. ROWE W. P., HUEBNER R. J., GILMORE L. K., PARROTT R. H., WARD T. G. Isolation of a cytopathogenic agent from human adenoids undergoing spontaneous degeneration in tissue culture. Proc Soc Exp Biol Med. 1953 Dec;84(3):570–573. doi: 10.3181/00379727-84-20714. [DOI] [PubMed] [Google Scholar]
  12. Reddick R. A., Lefkowitz S. S. In vitro immune responses of rabbits with persistent adenovirus type 5 infection. J Immunol. 1969 Oct;103(4):687–694. [PubMed] [Google Scholar]
  13. STROHL W. A., SCHLESINGER R. W. QUANTITATIVE STUDIES OF NATURAL AND EXPERIMENTAL ADENOVIRUS INFECTIONS OF HUMAN CELLS. I. CHARACTERISTICS OF VIRAL MULTIPLICATION IN FIBROBLASTS DERIVED BY LONG-TERM CULTURE FROM TONSILS. Virology. 1965 Jun;26:199–207. doi: 10.1016/0042-6822(65)90047-4. [DOI] [PubMed] [Google Scholar]
  14. STROHL W. A., SCHLESINGER R. W. QUANTITATIVE STUDIES OF NATURAL AND EXPERIMENTAL ADENOVIRUS INFECTIONS OF HUMAN CELLS. II. PRIMARY CULTURES AND THE POSSIBLE ROLE OF ASYNCHRONOUS VIRAL MULTIPLICATION IN THE MAINTENANCE OF INFECTION. Virology. 1965 Jun;26:208–220. doi: 10.1016/0042-6822(65)90048-6. [DOI] [PubMed] [Google Scholar]
  15. van der Veen J., Lambriex M. Relationship of adenovirus to lymphocytes in naturally infected human tonsils and adenoids. Infect Immun. 1973 Apr;7(4):604–609. doi: 10.1128/iai.7.4.604-609.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES