Abstract
Virulent Treponema pallidum has been shown to consume O2 at a rate similar to that of the known aerobic spirochaete, Leptospira. Such O2 uptake is cyanide sensitive, indicating a functioning cytochrome oxidase. Inhibition of O2 uptake by azide, chlorpromazine, and amytal further suggests a functioning electron transport system for the oxidation of nicotinamide adenine dinucleotide (reduced) to O2. Evidence is consistent with the probability that this terminal electron-transport system is coupled to oxidative phosphorylation. The potential significance of these findings is discussed.
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Selected References
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- Baseman J. B., Cox C. D. Terminal electron transport in Leptospira. J Bacteriol. 1969 Mar;97(3):1001–1004. doi: 10.1128/jb.97.3.1001-1004.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Biggins J., Dietrich W. E., Jr Respiratory mechanisms in the Flexibacteriaceae. I. Studies on the terminal oxidase system of Leucothrix mucor. Arch Biochem Biophys. 1968 Oct;128(1):40–50. doi: 10.1016/0003-9861(68)90007-6. [DOI] [PubMed] [Google Scholar]
- DAWKINS M. J., JUDAH J. D., REES K. R. The mechanism of action of chlorpromazine. Reduced diphosphopyridine nucleotidecytochrome c reductase and coupled phosphorylation. Biochem J. 1959 Sep;73:16–23. doi: 10.1042/bj0730016. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Eagle H., Steinman H. G. The Nutritional Requirements of Treponemata: I. Arginine, Acetic Acid, Sulfur-containing Compounds, and Serum Albumin as Essential Growth-promoting Factors for the Reiter Treponeme. J Bacteriol. 1948 Aug;56(2):163–176. [PMC free article] [PubMed] [Google Scholar]
- HARDY P. H., Jr, NELL E. E. Specific agglutination of Treponema pallidum by sera from rabbits and human beings with treponemal infections. J Exp Med. 1955 Apr 1;101(4):367–382. doi: 10.1084/jem.101.4.367. [DOI] [PMC free article] [PubMed] [Google Scholar]
- HOLLANDER D. H., NELL E. E. Improved preservation of Treponema pallidum and other bacteria by freezing with glycerol. Appl Microbiol. 1954 May;2(3):164–170. doi: 10.1128/am.2.3.164-170.1954. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hardy P. H., Jr, Nell E. E. A study of the properties of sorbent, the reagent employed in the fluorescent treponemal antibody-absorption test. Am J Epidemiol. 1972 Aug;96(2):141–152. doi: 10.1093/oxfordjournals.aje.a121440. [DOI] [PubMed] [Google Scholar]
- Hespell R. B., Canale-Parola E. Carbohydrate metabolism in Spirochaeta stenostrepta. J Bacteriol. 1970 Jul;103(1):216–226. doi: 10.1128/jb.103.1.216-226.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Nell E. E., Hardy P. H., Jr The use of freeze-preserved treponemes in the Treponema pallidum immobilization test. Cryobiology. 1972 Oct;9(5):404–410. doi: 10.1016/0011-2240(72)90157-5. [DOI] [PubMed] [Google Scholar]
- PORTNOY J., BREWER J. H., HARRIS A. Rapid plasma reagin card test for syphilis and other treponematoses. Public Health Rep. 1962 Aug;77:645–652. [PMC free article] [PubMed] [Google Scholar]
- PUMPHREY A. M., REDFEARN E. R. INHIBITION OF SUCCINATE OXIDATION BY BARBITURATES IN TIGHTLY COUPLED MITOCHONDRIA. Biochim Biophys Acta. 1963 Aug 13;74:317–327. doi: 10.1016/0006-3002(63)91375-1. [DOI] [PubMed] [Google Scholar]
- Robinson J., Cooper J. M. Method of determining oxygen concentrations in biological media, suitable for calibration of the oxygen electrode. Anal Biochem. 1970 Feb;33(2):390–399. doi: 10.1016/0003-2697(70)90310-6. [DOI] [PubMed] [Google Scholar]
- Staneck J. L., Henneberry R. C., Cox C. D. Growth requirements of pathogenic Leptospira. Infect Immun. 1973 Jun;7(6):886–897. doi: 10.1128/iai.7.6.886-897.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
- WEBER M. M. Factors influencing the in vitro survival of Treponema pallidum. Am J Hyg. 1960 May;71:401–417. doi: 10.1093/oxfordjournals.aje.a120123. [DOI] [PubMed] [Google Scholar]