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. 1974 Jul;10(1):173–179. doi: 10.1128/iai.10.1.173-179.1974

Biological Properties of Two Strains of Simian Virus 40 Isolated from Patients with Progressive Multifocal Leukoencephalopathy

Opendra Narayan 1, L P Weiner 1
PMCID: PMC414974  PMID: 4367120

Abstract

Biological properties of two strains of simian virus 40 (SV40) from brains of two patients with progressive multifocal leukoencephalopathy (PML) have been compared to those of a standard laboratory strain of SV40. Infectivity of both SV40-PML viruses was resistant to treatment with chloroform, low pH, and 50 C for 120 min. African green monkey kidney and BSC-1 cells were the most sensitive for viral replication, and cytopathology in these cultures was indistinguishable from that caused by SV40. Both viruses formed plaques in these cells. but, in African green monkey kidney cells, strain 1 virus produced plaques measuring 2 mm in diameter whereas strain 2 virus produced pleomorphic plaques varying from 1 to 10 mm in diameter. Hamster cells were not permissive for viral replication, and infection resulted only in viral transformation. Inoculation of human fetal glial cells resulted in a permissive lytic infection of one cell type and a persistent infection with only partial expression of the viral genome in the other. No morphological evidence of transformation was evident in the latter cells. Both strains of SV40-PML viruses were neutralized by commercial anti-SV40 serum, but in reciprocal kinetic neutralization tests differences in K values were noted when each was compared to SV40. Both viruses showed oncogenicity for hamsters, producing undifferentiated sarcomas when injected subcutaneously and choroid plexus papillomas after intracerebral inoculation. All hamster tumor cells contained intranuclear immunofluorescent tumor antigen. This was indistinguishable from SV40 T antigen in reciprocal staining reactions using hamster anti-T antibody induced by the two SV40-PML agents and SV40. These two human agents appear therefore to be new variants of simian virus 40.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Black P. H. The oncogenic DNA viruses: a review of in vitro transformation studies. Annu Rev Microbiol. 1968;22:391–426. doi: 10.1146/annurev.mi.22.100168.002135. [DOI] [PubMed] [Google Scholar]
  2. Butel J. S., Melnick J. L. Recent advances in molecular pathology. The state of the viral genome in cells transformed by simian virus 40: a review. Exp Mol Pathol. 1972 Aug;17(1):103–119. doi: 10.1016/0014-4800(72)90061-5. [DOI] [PubMed] [Google Scholar]
  3. FELDMAN H. A., WANG S. S. Sensitivity of various viruses to chloroform. Proc Soc Exp Biol Med. 1961 Apr;106:736–738. doi: 10.3181/00379727-106-26459. [DOI] [PubMed] [Google Scholar]
  4. Gardner S. D., Field A. M., Coleman D. V., Hulme B. New human papovavirus (B.K.) isolated from urine after renal transplantation. Lancet. 1971 Jun 19;1(7712):1253–1257. doi: 10.1016/s0140-6736(71)91776-4. [DOI] [PubMed] [Google Scholar]
  5. Neff J. M., Enders J. F. Poliovirus replication and cytopathogenicity in monolayer hamster cell cultures fused with beta propiolactone-inactivated Sendai virus. Proc Soc Exp Biol Med. 1968 Jan;127(1):260–267. doi: 10.3181/00379727-127-32668. [DOI] [PubMed] [Google Scholar]
  6. Padgett B. L., Walker D. L., ZuRhein G. M., Eckroade R. J., Dessel B. H. Cultivation of papova-like virus from human brain with progressive multifocal leucoencephalopathy. Lancet. 1971 Jun 19;1(7712):1257–1260. doi: 10.1016/s0140-6736(71)91777-6. [DOI] [PubMed] [Google Scholar]
  7. Penney J. B., Jr, Narayan O. Studies of the antigenic relationships of the new human papovaviruses by electron microscopy agglutination. Infect Immun. 1973 Aug;8(2):299–300. doi: 10.1128/iai.8.2.299-300.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. SWEET B. H., HILLEMAN M. R. The vacuolating virus, S.V. 40. Proc Soc Exp Biol Med. 1960 Nov;105:420–427. doi: 10.3181/00379727-105-26128. [DOI] [PubMed] [Google Scholar]
  9. Shein H. M. Propagation of human fetal spongioblasts and astrocytes in dispersed cell cultures. Exp Cell Res. 1965 Dec;40(3):554–569. doi: 10.1016/0014-4827(65)90234-x. [DOI] [PubMed] [Google Scholar]
  10. Shein H. M. Transformation of astrocytes and destruction of spongioblasts induced by a simian tumor virus (SV40) in cultures of human fetal neuroglia. J Neuropathol Exp Neurol. 1967 Jan;26(1):60–76. doi: 10.1097/00005072-196701000-00005. [DOI] [PubMed] [Google Scholar]
  11. Takemoto K. K., Kirschstein R. L., Habel K. Mutants of simian virus 40 differing in plaque size, oncogenicity, and heat sensitivity. J Bacteriol. 1966 Oct;92(4):990–994. doi: 10.1128/jb.92.4.990-994.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Takemoto K. K., Martin M. A. SV40 thermosensitive mutant: synthesis of viral DNA and virus-induced proteins at nonpermissive temperature. Virology. 1970 Dec;42(4):938–945. doi: 10.1016/0042-6822(70)90342-9. [DOI] [PubMed] [Google Scholar]
  13. Walker D. L., Padgett B. L., ZuRhein G. M., Albert A. E., Marsh R. F. Human papovavirus (JC): induction of brain tumors in hamsters. Science. 1973 Aug 17;181(4100):674–676. doi: 10.1126/science.181.4100.674. [DOI] [PubMed] [Google Scholar]
  14. Weiner L. P., Herndon R. M., Narayan O., Johnson R. T. Further studies of a simian virus 40-like virus isolated from human brain. J Virol. 1972 Jul;10(1):147–149. doi: 10.1128/jvi.10.1.147-149.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Weiner L. P., Herndon R. M., Narayan O., Johnson R. T., Shah K., Rubinstein L. J., Preziosi T. J., Conley F. K. Isolation of virus related to SV40 from patients with progressive multifocal leukoencephalopathy. N Engl J Med. 1972 Feb 24;286(8):385–390. doi: 10.1056/NEJM197202242860801. [DOI] [PubMed] [Google Scholar]

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