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. 1974 Jul;10(1):251–256. doi: 10.1128/iai.10.1.251-256.1974

Growth of Murine Cytomegalovirus in a Heterologous Cell System and Its Enhancement by 5-Iodo-2′-Deoxyuridine

G Plummer a,1, C R Goodheart b
PMCID: PMC414985  PMID: 4366921

Abstract

Mouse cytomegalovirus replicated in rabbit kidney cultures, a cell system of nonrodent origin. However, the sensitivity of these cultures, and the yields of virus therefrom, were lower than those of mouse cultures. Although a cytopathic effect developed in rabbit kidney cultures inoculated with sufficient amounts of the virus, such cultures were unsatisfactory for plaque assay. This was also true when rabbit fibroblast cultures were used, even though the murine cytomegalovirus replicated much better in mouse fibroblasts than in mouse kidney cultures, the latter of which contained extensive areas of epithelial cells. Viral growth in rabbit kidney cells was considerably enhanced when those cells had been initiated and grown in the presence of 5-iodo-2′-deoxyuridine; not only were the viral titers increased, but also the clarity and distinctness of the inclusion bodies.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Fioretti A., Furukawa T., Santoli D., Plotkin S. A. Nonproductive infection of guinea pig cells with human cytomegalovirus. J Virol. 1973 Jun;11(6):998–1003. doi: 10.1128/jvi.11.6.998-1003.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Goodheart C. R., Plummer G., Waner J. L. Density difference of DNA of human herpes simplex viruses, types I and II. Virology. 1968 Jul;35(3):473–475. doi: 10.1016/0042-6822(68)90225-0. [DOI] [PubMed] [Google Scholar]
  3. Hinze H. C. New member of the herpesvirus group isolated from wild cottontail rabbits. Infect Immun. 1971 Feb;3(2):350–354. doi: 10.1128/iai.3.2.350-354.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Kim K. S., Carp R. I. Growth of murine cytomegalovirus in various cell lines. J Virol. 1971 Jun;7(6):720–725. doi: 10.1128/jvi.7.6.720-725.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Nahmias A. J. Herpesviruses from fish to man--a search for pathobiologic unity. Pathobiol Annu. 1972;2:153–182. [PubMed] [Google Scholar]
  6. Raynaud J., Atanasiu P., Barreau C., Jahkola M. Adaptation d'un virus cytomégalique provenant du mulot (Apodemus sylvaticus) sur différentes cellules hétéroloques, y compris les cellules humaines. C R Acad Sci Hebd Seances Acad Sci D. 1969 Jul 7;269(1):104–106. [PubMed] [Google Scholar]
  7. St Jeor S., Rapp F. Cytomegalovirus replication in cells pretreated with 5-iodo-2'-deoxyuridine. J Virol. 1973 Jun;11(6):986–990. doi: 10.1128/jvi.11.6.986-990.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. St Jeor S., Rapp F. Cytomegalovirus: conversion of nonpermissive cells to a permissive state for virus replication. Science. 1973 Sep 14;181(4104):1060–1061. doi: 10.1126/science.181.4104.1060. [DOI] [PubMed] [Google Scholar]
  9. Waner J. L., Weller T. H. Behavior of human cytomegaloviruses in cell cultures of bovine and simian origin. Proc Soc Exp Biol Med. 1974 Feb;145(2):379–384. doi: 10.3181/00379727-145-37814. [DOI] [PubMed] [Google Scholar]

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