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. 1974 Aug;10(2):304–308. doi: 10.1128/iai.10.2.304-308.1974

Extracellular Polysaccharides of Actinomyces viscosus

Burton Rosan 1, Benjamin F Hammond 1
PMCID: PMC414996  PMID: 4851986

Abstract

Polysaccharide(s) have been isolated from supernatants of broth cultures of Actinomyces viscosus, strain T6. The organism produces significant quantities (2 mg/mg of cell [dry weight], 108 mg/liter) of this polysaccharide in the absence of sucrose. Chemical analysis indicated that N-acetylglucosamine (62%) was the major component; galactose (7%), glucose (4%), uronic acid (3%), and smaller amounts of glycerol, rhamnose, arabinose, and xylose were also present. The polysaccharide(s) is only slightly soluble in aqueous solutions. Although further purification of the polysaccharides will be necessary before definitive chemical and structural studies, the formation of extracellular polysaccharides is significant because it offers a possible explanation for plaque formation by these organisms.

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Selected References

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  1. BARKER S. A., BOURNE E. J., WHIFFEN D. H. Use of infrared analysis in the determination of carbohydrate structure. Methods Biochem Anal. 1956;3:213–245. doi: 10.1002/9780470110195.ch7. [DOI] [PubMed] [Google Scholar]
  2. Bladen H., Hageage G., Pollock F., Harr R. Plaque formation in vitro on wires by gram-negative oral microorganisms (Veillonella). Arch Oral Biol. 1970 Feb;15(2):127–133. doi: 10.1016/0003-9969(70)90048-8. [DOI] [PubMed] [Google Scholar]
  3. DISCHE Z. New color reactions for determination of sugars in polysaccharides. Methods Biochem Anal. 1955;2:313–358. doi: 10.1002/9780470110188.ch11. [DOI] [PubMed] [Google Scholar]
  4. Gibbons R. J., van Houte J. On the formation of dental plaques. J Periodontol. 1973 Jun;44(6):347–360. doi: 10.1902/jop.1973.44.6.347. [DOI] [PubMed] [Google Scholar]
  5. Griggs L. J., Post A., White E. R., Finkelstein J. A., Moeckel W. E., Holden K. G., Zarembo J. E., Weisbach J. A. Identification and quantitation of alditol acetates of neutral and amino sugars from mucins by automated gas-liquid chromatography. Anal Biochem. 1971 Oct;43(2):369–381. doi: 10.1016/0003-2697(71)90266-1. [DOI] [PubMed] [Google Scholar]
  6. HAMMOND B. F., ROSAN B., WILLIAMS N. B. ENCAPSULATED LACTOBACILLI. II. SPECIFIC CAPSULAR REACTION OF LACTOBACILLUS CASEI. J Bacteriol. 1964 Dec;88:1807–1808. doi: 10.1128/jb.88.6.1807-1808.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Howell A., Jr, Jordan H. V., Georg L. K., Pine L. Odontomyces viscosus, gen. nov., spec. nov., a filamentous microorganism isolated from periodontal plaque in hamsters. Sabouraudia. 1965 Jun;4(2):65–68. doi: 10.1080/00362176685190181. [DOI] [PubMed] [Google Scholar]
  8. JANCZURA E., PERKINS H. R., ROGERS H. J. Teichuronic acid: a mucopolysaccharide present in wall preparations from vegetative cells of Bacillus subtilis. Biochem J. 1961 Jul;80:82–93. doi: 10.1042/bj0800082. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. JORDAN H. V., KEYES P. H. AEROBIC, GRAM-POSITIVE, FILAMENTOUS BACTERIA AS ETIOLOGIC AGENTS OF EXPERIMENTAL PERIODONTAL DISEASE IN HAMSTERS. Arch Oral Biol. 1964 Jul-Aug;9:401–414. doi: 10.1016/0003-9969(64)90025-1. [DOI] [PubMed] [Google Scholar]
  10. Jordan H. V., Keyes P. H., Lim S. Plaque formation and implantation of Odontomyces viscosus in hamsters fed different carbohydrates. J Dent Res. 1969 Sep-Oct;48(5):824–831. doi: 10.1177/00220345690480053601. [DOI] [PubMed] [Google Scholar]
  11. Krichevsky M. I., Howell A., Jr, Lim S. Levan formation by Odontomyces viscosus. J Dent Res. 1969 Sep-Oct;48(5):938–942. doi: 10.1177/00220345690480055701. [DOI] [PubMed] [Google Scholar]
  12. LUDOWIEG J., DORFMAN A. A micromethod for the colorimetric determination of N-acetyl groups in acid mucopolysaccharides. Biochim Biophys Acta. 1960 Feb 26;38:212–218. doi: 10.1016/0006-3002(60)91233-6. [DOI] [PubMed] [Google Scholar]
  13. Reed M. J. Chemical and antigenic properties of the cell wall of Actinomyces viscosus (Strain T6). J Dent Res. 1972 Sep-Oct;51(5):1193–1202. doi: 10.1177/00220345720510050501. [DOI] [PubMed] [Google Scholar]
  14. Rosan B. Antigens of Streptococcus sanguis. Infect Immun. 1973 Feb;7(2):205–211. doi: 10.1128/iai.7.2.205-211.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Rosan B. Determination of muramic acid in automated amino acid analysis. Anal Biochem. 1972 Aug;48(2):624–628. doi: 10.1016/0003-2697(72)90119-4. [DOI] [PubMed] [Google Scholar]
  16. Socransky S. S., Hubersak C., Propas D. Induction of periodontal destruction in gnotobiotic rats by a human oral strain of Actinomyces naeslundii. Arch Oral Biol. 1970 Oct;15(10):993–995. doi: 10.1016/0003-9969(70)90095-6. [DOI] [PubMed] [Google Scholar]
  17. TOLKSDORF S. The in vitro determination of hyaluronidase. Methods Biochem Anal. 1954;1:425–457. doi: 10.1002/9780470110171.ch15. [DOI] [PubMed] [Google Scholar]
  18. Thompson J. S., Shockman G. D. A modification of the Park and Johnson reducing sugar determination suitable for the assay of insoluble materials: its application to bacterial cell walls. Anal Biochem. 1968 Feb;22(2):260–268. doi: 10.1016/0003-2697(68)90315-1. [DOI] [PubMed] [Google Scholar]
  19. van der Hoeven J. S. A slime-producing microorganism in dental plaque of rats, selected by glucose feeding. Chemical composition of extracellular slime elaborated by Actinomyces viscosus, strain Nyl. Caries Res. 1974;8(3):193–210. doi: 10.1159/000260109. [DOI] [PubMed] [Google Scholar]

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