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. 1974 Aug;10(2):352–355. doi: 10.1128/iai.10.2.352-355.1974

Dithiothreitol-Dependent Antilisterial Activity of Lysates from Normal Macrophages Exposed In Vitro to Culture Fluids from Spleen Cells of BCG-Immunized Mice 1

Donna Y Muirhead a, Virginia H Mallmann a
PMCID: PMC415004  PMID: 4211935

Abstract

The effect of culture fluids from mouse lymphocytes on the antilisterial activity of normal macrophages in vitro was determined. Titers of lysates of macrophages incubated with or without culture fluids from lymphocytes from control mice had titers of 10 to 270 with or without a reducing agent, dithiothreitol. Lysates of macrophages after incubation with culture fluids of spleen lymphocytes from BCG-immunized mice had antilisterial titers with and without dithiothreitol of 197,000 and 810, respectively. This may indicate a dithiothreitol-dependent antilisterial system inherent in activated macrophages of cell-mediated immunity. Lysates of incubated spleen cells and their culture fluids also had slight antilisterial activity.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Blanden R. V., Lefford M. J., Mackaness G. B. The host response to Calmette-Guérin bacillus infection in mice. J Exp Med. 1969 May 1;129(5):1079–1107. doi: 10.1084/jem.129.5.1079. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Howard D. H., Otto V., Gupta R. K. Lymphocyte-mediated cellular immunity in histoplasmosis. Infect Immun. 1971 Nov;4(5):605–610. doi: 10.1128/iai.4.5.605-610.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Klun C. L., Youmans G. P. The effect of lymphocyte supernatant fluids on the intracellular growth of virulent tubercle bacilli. J Reticuloendothel Soc. 1973 Mar;13(3):263–274. [PubMed] [Google Scholar]
  4. Klun C. L., Youmans G. P. The induction by Listeria monocytogenes and plant mitogens of lymphocyte supernatant fluids which inhibit the growth of Mycobacterium tuberculosis within macrophages in vitro. J Reticuloendothel Soc. 1973 Mar;13(3):275–285. [PubMed] [Google Scholar]
  5. Nathan C. F., Karnovsky M. L., David J. R. Alterations of macrophage functions by mediators from lymphocytes. J Exp Med. 1971 Jun 1;133(6):1356–1376. doi: 10.1084/jem.133.6.1356. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Patterson R. J., Youmans G. P. Demonstration in tissue culture of lymphocyte-mediated immunity to tuberculosis. Infect Immun. 1970 Jun;1(6):600–603. doi: 10.1128/iai.1.6.600-603.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Pearsall N. N., Sundsmo J. S., Weiser R. S. Lymphokine toxicity for yeast cells. J Immunol. 1973 May;110(5):1444–1446. [PubMed] [Google Scholar]

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