A CD8 T-Cell Epitope Variant Enhances Immune Targeting to a Recombinant Picornavirus Vaccine Antigen

Abstract

Recombinant virus vaccines are often less effective due to immunodominant responses against endogenous vector antigens. However, the use of small RNA virus vectors provides an opportunity to limit host exposure to endogenous virus antigens and focus immune responses on the desired vaccine antigen. Using the Daniel's strain of Theiler's murine encephalomyelitis virus, we have identified strategies to modulate responses to endogenous viral proteins by manipulating the host CD8+ T-cell repertoire prior to infection or through the use of mutations introduced into the virus genome. Both of these approaches enhance responses to vaccine antigens introduced into the picornavirus. However, the use of mutant immunodominant epitopes provides an opportunity for enhancing vaccine responses without further manipulation of the host. Using this strategy, we demonstrate that modification of the consensus MHC class I anchor residue within the virus genome can promote enhanced immunity to foreign antigens and self-antigens embedded in the virus genome.

Live virus vaccines have proven to be effective for driving CD8+ T-cell responses for therapy to treat a variety of diseases, making them appealing as vectors for antigen-specific immunotherapy (14,16,24,28,30). One of the major limitations to their efficacy, however, is the induction of immunity to vector antigens rather than recombinant target antigens (26,29). Competition between embedded and endogenous virus antigens limits the effectiveness of the vaccine response (9), decreasing their potential as antigen-specific therapy.

We have developed Theiler's murine encephalomyelitis virus (TMEV) as an attenuated live virus therapy that induces antigen-specific CD8+ T-cells against melanoma and breast cancer (21,22). As with other vectors, TMEV drives an immunodominant CD8+ T-cell response to an endogenous MHC class I peptide antigen, VP2_121–130 (18). When presented in the context of H-2D^b, CD8+ T-cells reactive to VP2_121–130 comprise up to 70% of the CD8+ T-cells recovered from infected mice (18). Although subdominant responses have been documented, the consequences of these responses and their impact on the overall response are minimal (15), suggesting that adaptive immune responses to the VP2_121–130 epitope may ultimately be exclusive for clearing and targeting virus-infected cells.

Although TMEV immunodominance provides a significant barrier to the development of this vector as a vaccine, the limited number of antigen specificities derived from this small virus makes engineering potential escape mutants less complex. We find that inhibition of this response using an H-2D^b mutant that does not present this epitope to CD8+ T-cells blocks the response to the VP2_121–130 peptide while enhancing vaccine responses (22). Since subverting immunodominance can lead to an enhanced response against subdominant epitopes (12,31), we asked whether the use of virus mutations identified within this TMEV epitope (2,25) could divert immunity away from the dominant epitope and increase responses to epitopes engineered within the attenuated virus, a method more amenable to clinical use.

Peptide Depletion Enhances the Proportion of Vaccine-Specific CD8+ T-Cells Using a TMEV Vector Expressing Ovalbumin Antigen

Previously, we had shown that antigen-specific depletion with VP2_121–130 peptide inhibits the expansion of the CD8+ T-cell response to wild-type virus. To determine whether this approach would enhance the response to an engineered vaccine strain, we infected mice with recombinant viruses after depletion of the immunodominant response using this approach. We intravenously delivered 300 μg of VP2_121–130 peptide (FHAGSLLVFM) or control peptide (HPV E7_49–57) to C57BL/6 mice 24 h prior to intracranial injection with 2×10⁴ PFU of recombinant TMEV encoding the OVA_257–265 model epitope (TMEV-OVA8). Infection of the brain allows the direct isolation of infiltrating T-cells at the site of infection, and provides an easily dissociated tissue that allows direct ex vivo analysis of activated cells (13). This approach has been used previously to demonstrate the efficacy of systemically delivered TMEV vaccines (21,22). Six days after infection, central nervous system infiltrating lymphocytes were analyzed by flow cytometry for the presence of virus-specific H-2D^b/VP2_121–130 or vaccine-targeted H-2K^b/OVA_257–265 CD8+ T-cells using MHC class I tetramers. We find that VP2₁₂₁ peptide delivery dramatically reduces the percentage of immunodominant virus reactive T-cells from the infected tissue site (Fig. 1A). This approach enhanced the percentage of OVA_257–265-specific T-cells generated compared to the control peptide depletion (Fig. 1B). However, it did not increase the overall quantity of vaccine-specific CD8+ T-cells (Fig. 1C). Further, elimination of VP2₁₂₁-specific T-cells did not affect the amount of virus RNA recovered after infection using attenuated TMEV-OVA8. However, levels of TMEV-OVA8 virus RNA were significantly reduced compared to infection with wild-type TMEV (Fig. 1D).

FIG. 1.

Qualitative enhancement of CD8+ vaccine responses using peptide inhibition of vector-specific T-cells. Central nervous system infiltrating lymphocytes were analyzed for the presence of CD8+ T-cells specific for virus antigen, as well as ovalbumin vaccine antigen after immunization with antigen encoding TMEV-OVA8. (A) H-2D^b/VP2_121–130-specific responses after control SV40 Lg T peptide or VP2_121–130 peptide administration. (B) H-2K^b/OVA_257–264-specific responses after control and VP2_121–130 peptide administration. (C) Left: Percent of the CD45+ infiltrating population that are H-2D^b/VP2_121–130 or H-2K^b/OVA_257–264 specific. Right: Number of CD45+ VP2₁₂₁+or OVA₂₅₇+cells (^ap<0.05, rank-sum test; *p<0.05, t-test). (D) Viral central nervous system RNA levels as measured by quantitative reverse transcription polymerase chain reaction from animals infected with TMEV viruses for 6 days with or without prior peptide treatment (*p<0.05 compared to all, t-test).

A Partial Escape Mutation Enhances the Antigen-Specific CD8+ T-Cell Response to a Recombinant TMEV Vaccine

Since manipulation of the CD8+ T-cell repertoire through the induction of nonresponsiveness can modify responses to vaccine antigens, we asked whether direct manipulation of the virus genome itself could reduce immunodominance and enhance responses to recombinant vaccine antigens. Previously, we and others had identified mutations within the VP2_121–130 coding sequence for TMEV, and found that immunodominance is less pronounced in infected animals using these virus mutants (2,25). Using an engineered TMEV vector system, we introduced the VP2-S125A and VP2-M130L mutations by site-directed mutagenesis into a cDNA that encodes the TMEV-OVA8 strain, a vaccine that drives H-2K^b restricted CD8+ T-cell responses to the model foreign peptide OVA₂₅₇ (21). Using this approach to derive new live virus vaccines, we infected C57BL/6 mice and recovered lymphocytes from infected tissues 6 days post-infection. We found that introduction of the VP2-M130L mutation enhances the response to the model epitope OVA₂₅₇. Both the percentage and number of antigen-specific CD8+ T-cells were increased using this approach (Fig. 2A–C). These mutations introduce a change that is predicted to destabilize the interaction of this peptide with the H-2D^b molecule (8) according to a peptide-binding algorithm (19). However, only the M130L mutation enhances immunity to the embedded vaccine epitope, suggesting that other factors influence the vaccine response. These factors might include the generation of new epitopes (1), enhanced responses to subdominant epitopes (15,31), or modulation of virus replication and assembly (2). We found an increase in the percent of CD8+ T-cells that are not specifically recognized by the OVA₂₅₇- or VP2₁₂₁-specific tetramers using the VP2-S125A mutant (Fig. 2C), supporting the potential for enhanced responses to off-target viral antigens. The affect cannot be explained by levels of virus, since the virus RNA levels reached after 6 days of infection were similar among the ovalbumen expressing viruses and were decreased compared to wild-type TMEV, demonstrating that the VP2 mutant viruses are similarly attenuated (Fig. 2D). Fortuitously, the M130L mutation provides a means of enhancing responses to vaccine epitopes in a manner that also decreases the reliance on the immunodominant CD8+ T-cell responses needed for virus clearance, since integration of epitopes within the virus leader sequence provides antigen as well as a mechanism for attenuation (21,22).

FIG. 2.

Mutagenesis of the immunodominant vector epitope VP2_121–130 promotes elevated CD8+ T-cell responses to an embedded vaccine antigen. CNS infiltrating lymphocytes derived from mice infected with antigen expressing vaccines containing the VP2-S125A and VP2-M130L mutations were evaluated by FACS for the presence of viral and OVA_257–264-specific CD8+ T-cells. (A) H-2D^b/VP2_121–130-specific responses after infection with VP2-S125A-OVA8 (n=4) and VP2-M130L-OVA8 (n=4) vaccines compared to TMEV-OVA8 (n=4) and wild-type TMEV (n=3). (B) H-2K^b/OVA_257–264-specific responses from the same samples in (A). (B) Quantitative assessment of the percentage and absolute numbers of CD8+cells specific for VP2_121–130 and OVA_257–264 (*p<0.05 compared to all others; ^ap<0.05 compared to M130L-OVA8 and DA-wt by ANOVA/Student/Newman–Keuls). (C) Distribution of the CD8+ T-cell response to ovalbumen expressing viruses. Other CD8+ T-cell responses represent the population of cells negative for VP2_121–130 and OVA_257–264 (*p<0.05 compared to all others; ANOVA/Student/Newman–Keuls). (D) Viral RNA levels from animals in (A) (*p<0.05 compared to all others; ANOVA/Student/Newman–Keuls). (E) Functional assessment of CD8+ T-cells harvested from mice challenged with ovalbumen and previously primed with vaccines containing the VP2-M130L mutation or wt-VP2. Splenocytes were gated on total CD8+ T-cells to analyze the percentage of CD44+ IFN-γ+cells after stimulation with OVA_257–264 or no peptide control (*p<0.05, ANOVA/Student/Newman–Keuls).

To demonstrate the functional utility of the VP2-M130L mutations, we intraperitoneally infected B6 mice with either 2×10⁵ PFU of wild-type TMEV-OVA8 or with VP2-M130L-OVA8 1 week prior to a second challenge with wild-type TMEV-OVA8 virus alone. Seven days after the second challenge, splenocytes were harvested for FACS analysis by intracellular IFN-γ staining (17). After re-stimulation with OVA₂₅₇ peptide, the animals primed with VP2-M130L-OVA8 had an enhancement in the percentage of CD44+ IFN-γ+ CD8 T-cells after stimulation by the cognate vaccine antigen (Fig. 2E), further demonstrating the utility of this vaccine for enhancing CD8+ T-cell immunity by increasing T-cell activation and IFN-γ production. In addition, the VP2-M130L mutation provides a tool for use in heterologous boost vaccines that can promote CD8+ T-cell immunity to epitopes embedded within TMEV.

Engineering a Virus Escape Mutation into TMEV Enhances the CD8+ T-Cell Response to the Breast Cancer Antigen HER2/neu in F1 Mice

Although the enhancement of vaccine responses can be accomplished using escape mutants, the activation of therapeutic CD8+ T-cell responses to treat chronic infection or cancer provides several unique challenges (7). We have previously shown the benefit of using a HER2/neu expressing TMEV vaccine for the treatment of breast tumors in Balb/c mice (21). This vaccine expresses the H-2K^d epitope p66 derived from the oncogene rat HER2/neu (20). Since the immunodominant response to the VP2_121–130 peptide is H-2D^b restricted, determining whether VP2 mutants can enhance responses to the H-2K^d restricted epitope requires the use of F1 hybrid mice. Therefore, we infected Balb/C57BL/6 F1 mice (Jackson Labs, Bar Harbor, ME) with this virus for 6 days, and assessed its effectiveness by determining the quality and quantity of p66 reactive CD8+ cells by FACS. We found that the introduction of the VP2-S125A mutation into the p66 wild-type vaccine does not appreciably enhance the response to the p66 antigen. In contrast, introduction of the VP2-M130L mutation increases the percentage and number of H-2K^d/p66 reactive cells (Fig. 3A and B), demonstrating that modification of the H-2D^b restricted antigen promotes the enhancement of H-2K^d restricted responses in an F1 strain expressing five unique MHC class I genes (H-2K^d, D^d, L^d, K^b, and D^b).

FIG. 3.

Diverting immunodominance promotes enhanced antitumor responses to an H-2K^d restricted HER2/neu epitope. Lymphocytes isolated after infection with VP2 mutagenized TMEV p66 vaccine were analyzed for the presence of CD8+ T-cells specific for the HER2/neu peptide p66. (A) H-2K^d/p66 specific CD8+ T-cells induced with TMEV p66 vaccines encoding VP2-S125A and M130L mutations compared to a vaccine with VP2 wild-type sequence and the nonvaccine strain wild-type TMEV. (B) Assessment of the percentage and absolute number of antigen specific CD8+ T-cells induced with p66 vaccines (*p<0.05 compared to specified or ^ap<0.05 significant compared to all others by ANOVA/Student/Newman–Keuls).

Our studies focused on the inhibition of the response to the VP2_121–130-specific CD8+ T-cell response to enhance responses to foreign or tumor antigens embedded within TMEV. This epitope has been studied extensively in mice expressing the MHC class I allele H-2D^b because of its importance for protection from viral persistence and demyelinating disease (2,3,11). However, less is known about its potential for binding other MHC alleles or the potential role that non-H-2D^b restricted T-cells might play in targeting this epitope. Previously, others have shown that expression of the VP2_121–130 peptide in H-2^q mice can modulate the extent of immune mediated pathology (6), suggesting a potential role for H-2^q restricted T-cells in modulating pathology and the possibility that the responses to this peptide play a role in other MHC haplotypes. In humans, nine major MHC class I supertypes have been identified based on their structural similarity in the peptide-binding cleft (27). These studies have demonstrated a degree of degeneracy in HLA peptide binding, suggesting that the principles identified in the current report could be applied to the development of vaccines that will be beneficial across a broad spectrum of MHC haplotypes (4,5,10).

Several RNA viruses are currently being developed as recombinant vaccine vectors (23). However, the benefits of using recombinant virus vaccines have been limited by several factors, including off-target immune responses directed toward endogenous virus antigens (26,29). Many of these recombinant vaccines encode large genomes with complex antigens that compete with or inhibit desired vaccine responses (29,31). We find that a small RNA virus can drive potent CD8+ T-cell responses to recombinant antigens and that manipulation of its genome can be used to enhance the safety of this vaccine, as well as modulate its immune profile. Here, we find that genetic modification of an immunodominant MHC class I epitope can be used to promote adaptive CD8+ T-cell responses to recombinant antigens, providing a strategy to enhance the potency of this vaccine and support for the development of small RNA viruses as therapeutic vectors.

Acknowledgments

This work was supported by grants from the National Institutes of Health (5R01CA104996-08 and 5R01NS060881-06; A.J.J.).

Author Disclosure Statement

No competing financial interests exist.