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. 1975 Jan;11(1):152–158. doi: 10.1128/iai.11.1.152-158.1975

Characterization of an in vitro persistent-state measles virus infection: establishment and virological characterization of the BGM/MV cell line.

J H Menna, A R Collins, T D Flanagan
PMCID: PMC415039  PMID: 803920

Abstract

The parameters of a persistent-state measles virus infection in BGM/MV cells were examined. The BGM/MV cell line was established by cocultivation of measles virus-infected primary C3H mouse brain cells with a stable line of African green monkey kidney cells (BGM). Initially, a morphologically mixed population of cells existed:BGM-like (epithelioid) and fibroblasts. Gradually the fibroblasts were replaced by BGM-like cells, resulting in a morphologically homogeneous population. Measles cytopathic effect was noted 2 days after initiation of this culture and persisted for approximately 290 days. The time of disappearance of viral cytopathic effect corresponded to the time at which morphological homogeneity was reached. Low titers of infectious measles virus were detected in the BGM/MV culture up to 20 days postseeding; thereafter none was observed. After 440 days in culture, 100% of BGM/MV cells demonstrated intractyoplasmic measles antigen by immunofluorescence. Nuclear fluorescence was never observed. Electron microscopy revealed the presence of measles virus mucleocapsid within the almost completely filling the cytoplasm of BGM/MV cells. The plasma membrane of these cells appeared normal; no maturing or budding particles were observed. Measles virus hemagglutinin was not detected in either clarified cell lysates or in supernatant culture fluids. Cell membrane alteration by measles virus was detected in less than 1% of these cells by hemadsorption and by membrane immunofluorescence. The hemadsorption activity of the cells could be enhanced (30 to 70%) by treatment with actinomycin D or enucleation with cytochalasin B; these treatments, however, were unsuccessful in inducing detectable levels of measles hemagglutinin. Treatment of BGM/MV cells with 5-bromo-2'-deoxyuridine (BUdR) at 5 to 50 mug/ml and cytosine arabinoside at 1 to 50 mug/ml failed to enhance hemadsorption activity. Doses of 5-bromo-2'-deoxyuridine ranging from 5 to 200 mug/ml and of actinomycin D ranging from 0.1 to 10 mug/ml were ineffective in inducing the synthesis of infectious virus. Various physical methods of induction of infectious virus was also unsuccessful.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Barron A. L., Olshevsky C., Cohen M. M. Characteristics of the BGM line of cells from African green monkey kidney. Brief report. Arch Gesamte Virusforsch. 1970;32(4):389–392. doi: 10.1007/BF01250067. [DOI] [PubMed] [Google Scholar]
  2. Bather R., Furesz J., Fanok A. G., Gill S. D., Yarosh W. Long term infection of diploid African green monkey brain cells by Schwarz measles vaccine virus. J Gen Virol. 1973 Sep;20(3):401–405. doi: 10.1099/0022-1317-20-3-401. [DOI] [PubMed] [Google Scholar]
  3. Flanagan T. D., Barron A. L. Plaque formation by mumps virus and inhibition by antiserum. Appl Microbiol. 1970 Feb;19(2):360–366. doi: 10.1128/am.19.2.360-366.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Gibson P. E., Bell T. M. Persistent infection of measles virus in mouse brain cell cultures infected in vivo. Arch Gesamte Virusforsch. 1972;37(1):45–53. doi: 10.1007/BF01241149. [DOI] [PubMed] [Google Scholar]
  5. IMAGAWA D. T., ADAMS J. M. Propagation of measles virus in suckling mice. Proc Soc Exp Biol Med. 1958 Jul;98(3):567–569. doi: 10.3181/00379727-98-24109. [DOI] [PubMed] [Google Scholar]
  6. Menna J. H., Collins A. R., Flanagan T. D. Characterization of an in vitro persistent-state measles virus infection: species characterization and interference in the BGM/MV cell line. Infect Immun. 1975 Jan;11(1):159–163. doi: 10.1128/iai.11.1.159-163.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Nakai M., Imagawa D. T. Electron microscopy of measles virus replication. J Virol. 1969 Feb;3(2):187–197. doi: 10.1128/jvi.3.2.187-197.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Prescott D. M., Myerson D., Wallace J. Enucleation of mammalian cells with cytochalasin B. Exp Cell Res. 1972;71(2):480–485. doi: 10.1016/0014-4827(72)90322-9. [DOI] [PubMed] [Google Scholar]
  9. ROSANOFF E. I. Hemagglutination and hemadsorption of measles virus. Proc Soc Exp Biol Med. 1961 Mar;106:563–567. doi: 10.3181/00379727-106-26403. [DOI] [PubMed] [Google Scholar]
  10. Rustigian R. Persistent infection of cells in culture by measles virus. II. Effect of measles antibody on persistently infected HeLa sublines and recovery of a HeLa clonal line persistently infected with incomplete virus. J Bacteriol. 1966 Dec;92(6):1805–1811. doi: 10.1128/jb.92.6.1805-1811.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. SEVER J. L. Application of a microtechnique to viral serological investigations. J Immunol. 1962 Mar;88:320–329. [PubMed] [Google Scholar]
  12. TAWARA J. FINE STRUCTURE OF FILAMENTS IN DOG KIDNEY CELL CULTURES INFECTED WITH MEASLES VIRUS. Virology. 1965 Feb;25:322–322. doi: 10.1016/0042-6822(65)90209-6. [DOI] [PubMed] [Google Scholar]
  13. WALKER D. L. THE VIRAL CARRIER STATE IN ANIMAL CELL CULTURES. Prog Med Virol. 1964;6:111–148. [PubMed] [Google Scholar]
  14. Winston S. H., Rustigian R., Bratt M. A. Persistent infection of cells in culture by measles virus. 3. Comparison of virus-specific RNA synthesized in primary persistent infection in HeLa cells. J Virol. 1973 Jun;11(6):926–932. doi: 10.1128/jvi.11.6.926-932.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]

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