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. 1975 Feb;11(2):231–239. doi: 10.1128/iai.11.2.231-239.1975

Identification of measles virus-specific hemolysis-inihibiting antibodies separate from hemagglutination-inhibiting antibodies.

E Norrby, Y Gollmar
PMCID: PMC415050  PMID: 803467

Abstract

The occurrence of antibodies giving hemolysis inhibition (HLI) but not hemagglutination inhibition (HI) was examined in human convalescent and rabbit hyperimmune sera. HI antibodies, which through their interaction with hemagglutinin components display HLI activity, were removed by absorption with Tween 80-ether (TE)-treated measles virus material. This absorption did not change the titer of non-HI HLI antibodies. After removal of HI antibodies from 16 late measles convalescent sera and three batches of gamma globulin. HLI antibody titers showed a two- to eightfold reduction. The titers of neutralizing antibodies were reduced from 1/4 to 1/20 of the original titers. There was a good correlation between the titers of neutralizing and HLI antibodies both in sera from which HI antibodies had been removed by absorption and in sera spontaneously showing markedly higher HLI than HI antibody titers. HLI antibodies with these characteristics could be identified in HI tests when whole virus instead of TE-treated material was used an antigen and anti-antiserum was added to the tests. In contrast to the situation in human sera, antibodies remaining after removal of HI antibodies from rabbit hyperimmune sera against purified virus particles were detectable in neutralization and HLI tests only in the presence of anti-antiserum. However, virus particles from which the major fraction of all envelope projections had been removed by treatment with 0.004% trypsin induced the production of non-HI HLI antibodies active also in the absence of anti-antiserum. TE and formalin treatment destroyed the hemolytic activity of virus preparations and also their capacity to induce a production of non-HI HLI antibodies.

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Selected References

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  1. Barbanti-Brodano G., Oyanagi S., Katz M., Koprowski H. Presence of 2 different viral agents in brain cells of patients with subacute sclerosing panencephalitis. Proc Soc Exp Biol Med. 1970 May;134(1):230–236. doi: 10.3181/00379727-134-34765. [DOI] [PubMed] [Google Scholar]
  2. Dowdle W. R., Sarateanu D., Reimer C. B. Antiantibody enhancement of hemagglutination inhibition by influenza anti-neuraminidase. J Immunol. 1972 Dec;109(6):1321–1326. [PubMed] [Google Scholar]
  3. Hall W. W., Martin S. J. The biochemical and biological characteristics of the surface components of measles virus. J Gen Virol. 1974 Mar;22(3):363–374. doi: 10.1099/0022-1317-22-3-363. [DOI] [PubMed] [Google Scholar]
  4. Howe C., Schluederberg A. Neurminidase associated with measles virus. Biochem Biophys Res Commun. 1970 Aug 11;40(3):606–607. doi: 10.1016/0006-291x(70)90946-0. [DOI] [PubMed] [Google Scholar]
  5. Kendal A. P., Madeley C. R. Flocculation of influenza virus by specific anti-neuraminidase antibody. Arch Gesamte Virusforsch. 1970;31(3):219–229. doi: 10.1007/BF01253756. [DOI] [PubMed] [Google Scholar]
  6. Klenk H. D., Caliguiri L. A., Choppin P. W. The proteins of the parainfluenza virus SV5. II. The carbohydrate content and glycoproteins of the virion. Virology. 1970 Oct;42(2):473–481. doi: 10.1016/0042-6822(70)90290-4. [DOI] [PubMed] [Google Scholar]
  7. NORRBY E. Hemagglutination by measles virus. 4. A simple procedure for production of high potency antigen for hemagglutination-inhibition (HI) tests. Proc Soc Exp Biol Med. 1962 Dec;111:814–818. doi: 10.3181/00379727-111-27930. [DOI] [PubMed] [Google Scholar]
  8. NORRBY E. Hemagglutination by measles virus. II. Properties of the hemagglutinin and of the receptors on the erythrocytes. Arch Gesamte Virusforsch. 1962;12:164–172. doi: 10.1007/BF01258127. [DOI] [PubMed] [Google Scholar]
  9. NORRBY E., LAGERCRANTZ R., GARD S. MEASLES VACCINATION. IV. RESPONSES TO TWO DIFFERENT TYPES OF PREPARATIONS GIVEN AS A FOURTH DOSE OF VACCINE. Br Med J. 1965 Mar 27;1(5438):813–817. [PMC free article] [PubMed] [Google Scholar]
  10. Norrby E., Gollmar Y. Appearance and persistence of antibodies against different virus components after regular measles infections. Infect Immun. 1972 Sep;6(3):240–247. doi: 10.1128/iai.6.3.240-247.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Norrby E., Hammarskjöld B. Structural components of measles virus. Microbios. 1972 Jan;5(17):17–29. [PubMed] [Google Scholar]
  12. Norrby E., Lagercrantz R., Gard S., Carlström G. Measles vaccination. 3. Serologic responses to immunization with purified hemagglutinin. Acta Paediatr Scand. 1965 Nov;54(6):581–586. doi: 10.1111/j.1651-2227.1965.tb06422.x. [DOI] [PubMed] [Google Scholar]
  13. Norrby E. The effect of a carbobenzoxy tripeptide on the biological activities of measles virus. Virology. 1971 Jun;44(3):599–608. doi: 10.1016/0042-6822(71)90374-6. [DOI] [PubMed] [Google Scholar]
  14. Norrby E., Wadell G. The relationship between the soluble antigens and the virion of adenovirus type 3. VI. Further characterization of antigenic sites available at the surface of virions. Virology. 1972 Jun;48(3):757–765. doi: 10.1016/0042-6822(72)90159-6. [DOI] [PubMed] [Google Scholar]
  15. ODDO F. G., FLACCOMIO R., SINATRA A. "Giant-cell" and "strand-forming" cytopathic effect of measles virus lines conditioned by serial propagation with diluted or concentrated inoculum. Virology. 1961 Apr;13:550–553. doi: 10.1016/0042-6822(61)90288-4. [DOI] [PubMed] [Google Scholar]
  16. Salmi A. A., Norrby E., Panelius M. Identification of different measles virus-specific antibodies in the serum and cerebrospinal fluid from patients with subacute sclerosing pancencephalitis and multiple sclerosis. Infect Immun. 1972 Sep;6(3):248–254. doi: 10.1128/iai.6.3.248-254.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Salmi A., Gollmar Y., Norrby E., Panelius M. Antibodies against three different structural components of measles virus in patients with multiple sclerosis, their siblings and matched controls. Acta Pathol Microbiol Scand B Microbiol Immunol. 1973 Oct;81(5):627–634. doi: 10.1111/j.1699-0463.1973.tb02253.x. [DOI] [PubMed] [Google Scholar]
  18. Scheid A., Caliguiri L. A., Compans R. W., Choppin P. W. Isolation of paramyxovirus glycoproteins. Association of both hemagglutinating and neuraminidase activities with the larger SV5 glycoprotein. Virology. 1972 Dec;50(3):640–652. doi: 10.1016/0042-6822(72)90418-7. [DOI] [PubMed] [Google Scholar]
  19. Scheid A., Choppin P. W. Identification of biological activities of paramyxovirus glycoproteins. Activation of cell fusion, hemolysis, and infectivity of proteolytic cleavage of an inactive precursor protein of Sendai virus. Virology. 1974 Feb;57(2):475–490. doi: 10.1016/0042-6822(74)90187-1. [DOI] [PubMed] [Google Scholar]

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