Abstract
The presence of antiglobulin factors in normal mouse serum was studied. Mice were injected with human 0 Rh(+) erythrocytes and the resulting gamma globulins were digested with papain. The reaction was demonstrated by agglutinations of human 0 Rh (+) perythryocytes sensitized with the Fab fragments when treated with normal mouse serum. Studies suggested that there are two factors in mice, a 7S AND A 19S globulin form. Cross-reactivity between strains of mice was demonstrated.
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- DRAY S., YOUNG G. O. Differences in the antigenic components of sera of individual rabbits as shown by induced isoprecipitins. J Immunol. 1958 Aug;81(2):142–149. [PubMed] [Google Scholar]
- Kormeier L. C., Ing J. T., Mandy W. J. Specificity of antiglobulin factors in normal human serum reacting with enzyme digested gamma-G-globulin. J Immunol. 1968 Mar;100(3):612–621. [PubMed] [Google Scholar]
- MILGROM F., DUBINSKI S. Antigenicity of antibodies of the same species. Nature. 1957 Jun 29;179(4574):1351–1352. doi: 10.1038/1791351b0. [DOI] [PubMed] [Google Scholar]
- Mandy W. J. A new serum factor in normal rabbits. 3. Specificity for antigenic determinants uncovered by papain or pepsin digestion. J Immunol. 1967 Oct;99(4):815–824. [PubMed] [Google Scholar]
- Mandy W. J. A new serum factor in normal rabbits. II. Reaction with buried antigenic determinants revealed after papain digestion. J Immunol. 1966 Dec;97(6):876–884. [PubMed] [Google Scholar]
- Mandy W. J., Fudenberg H. H., Lewis F. B. A new serum factor in normal rabbits. I. Identification and characterization. J Immunol. 1965 Sep;95(3):501–509. [PubMed] [Google Scholar]
- Merryman C., Frangione B., Franklin E. C., Benacerraf B. Peptide differences in purified mouse antibodies. Immunochemistry. 1967 Jan;4(1):31–36. doi: 10.1016/0019-2791(67)90194-2. [DOI] [PubMed] [Google Scholar]
- PORTER R. R. The hydrolysis of rabbit y-globulin and antibodies with crystalline papain. Biochem J. 1959 Sep;73:119–126. doi: 10.1042/bj0730119. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Potter M., Lieberman R. Genetics of immunoglobulins in the mouse. Adv Immunol. 1967;7:91–145. doi: 10.1016/s0065-2776(08)60127-3. [DOI] [PubMed] [Google Scholar]
- Richie E. R., Woolsey M. E., Mandy W. J. A new serum factor in normal rabbits. VI. Reaction with buried determinants of IgG exposed sequentially with CNBr, pepsin and papain. J Immunol. 1970 Apr;104(4):984–991. [PubMed] [Google Scholar]