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. 1975 Feb;11(2):415–416. doi: 10.1128/iai.11.2.415-416.1975

Evaluation of immunological specificity of flurescein-conjugated antisera with agarose-antigen sections.

J B Case, L M Lussier, M Mannik
PMCID: PMC415078  PMID: 803474

Abstract

Purified immunoglobulins were conjugated to agarose beads; the beads were frozen, and used to establish the specificity of fluorescein-conjugated antisera.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ALLEN J. C. IMMUNOFLUORESCENCE APPLIED TO PROTEIN SOLUTIONS. J Lab Clin Med. 1963 Sep;62:517–524. [PubMed] [Google Scholar]
  2. Bergquist N. R., Kreisler M. E. The use of polymerized immunoglobulin particles for specificity control of fluorescent conjugates. J Immunol Methods. 1974 Mar;4(2):195–202. doi: 10.1016/0022-1759(74)90061-1. [DOI] [PubMed] [Google Scholar]
  3. Hijmans W., Schuit H. R., Klein F. An immunofluorescence procedure for the detection of intracellular immunoglobulins. Clin Exp Immunol. 1969 Apr;4(4):457–472. [PMC free article] [PubMed] [Google Scholar]
  4. Mannik M., Stage D. E. Antibody-agarose immunoadsorbents: complete removal of classes of immunoglobulins from serum. J Immunol. 1971 Jun;106(6):1670–1672. [PubMed] [Google Scholar]
  5. PARONETTO F. The fluorescent antibody technique applied to titration and identification of antigens in solutions or antisera. Proc Soc Exp Biol Med. 1963 Jun;113:394–397. doi: 10.3181/00379727-113-28375. [DOI] [PubMed] [Google Scholar]
  6. Stage D. E., Mannik M. Covalent binding of molecules to CNBr-activated agarose: parameters relevant to the activation and coupling reactions. Biochim Biophys Acta. 1974 Apr 22;343(2):382–391. doi: 10.1016/0304-4165(74)90102-0. [DOI] [PubMed] [Google Scholar]
  7. van Dalen J. P., Knapp W., Ploem J. S. Microfluorometry on antigen-antibody interaction in immunofluorescence using antigens covalently bound to agarose beads. J Immunol Methods. 1973 Jun;2(4):383–392. doi: 10.1016/0022-1759(73)90004-5. [DOI] [PubMed] [Google Scholar]

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