Skip to main content
PMC home page
Infection and Immunity logoLink to Infection and Immunity
. 1975 Apr;11(4):804–808. doi: 10.1128/iai.11.4.804-808.1975

Persistence of rubellavirus-specific immunoglobulin M and immunoglobulin A antibodies: investigation of successive serum samples with lowered immunoglobulin G concentration.

A Hornsleth, J Leerhoy, P Grauballe, H Spanggaard
PMCID: PMC415138  PMID: 1091553

Abstract

The persistence of rubellavirus-specific immunoglobulin (Ig) M and IgA antibodies has been studied in seven patients with primary postnatal rubella infections. Successive blood samples obtained over a period of several years after the onset of disease have been investigated, employing the fluorescent antibody technique and the sucrose gradient centrifugation method. IgM antibodies were found to persist for 4 to 5 weeks after the onset of disease, with only moderate variation being observed with respect to the different patients and the method of investigation being studied. The persistence of IgA antibodies (as examined by the fluorescent antibody technique) varied from a few weeks to several years after the onset of the disease. The detection of IgA antibodies cannot be considered as conclusive for the diagnosis of recent rubella infections.

Full text

PDF
804

Images in this article

805Image
on p.805

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Banatvala J. E., Best J. M., Waller D. K. Epstein-Barr virus-specific IgM in infectious mononucleosis, Burkitt lymphoma, nasopharyngeal carcinoma. Lancet. 1972 Jun 3;1(7762):1205–1208. doi: 10.1016/s0140-6736(72)90925-7. [DOI] [PubMed] [Google Scholar]
  2. Best J. M., Banatvala J. E., Watson D. Serum IgM and IgG responses in postnatally acquired rubella. Lancet. 1969 Jul 12;2(7611):65–68. doi: 10.1016/s0140-6736(69)92386-1. [DOI] [PubMed] [Google Scholar]
  3. Cradock-Watson J. E., Bourne M. S., Vandervelde E. M. IgG, IgA and IgM responses in acute rubella determined by the immunofluorescent technique. J Hyg (Lond) 1972 Sep;70(3):473–485. doi: 10.1017/s0022172400063063. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Cradock-Watson J. E., Ridehalgh M. K., Bourne M. S., Vandervelde E. M. Nasal immunoglobulin responses in acute rubella determined by the immunofluorescent technique. J Hyg (Lond) 1973 Sep;71(3):603–617. doi: 10.1017/s002217240004660x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Desmyter J. Absence of non-acute rubella IgM antibodies in chronic hepatitis and rheumatoid arthritis. Lancet. 1972 Nov 25;2(7787):1141–1142. doi: 10.1016/s0140-6736(72)92740-7. [DOI] [PubMed] [Google Scholar]
  6. Desmyter J., South M. A., Rawls W. E. The IgM antibody response in rubella during pregnancy. J Med Microbiol. 1971 Feb;4(1):107–114. doi: 10.1099/00222615-4-1-107. [DOI] [PubMed] [Google Scholar]
  7. Field P. R., Murphy A. M. The role of specific IgM globulin estimations in the diagnosis of acquired rubella. Med J Aust. 1972 Nov 25;2(22):1244–1248. doi: 10.5694/j.1326-5377.1972.tb47544.x. [DOI] [PubMed] [Google Scholar]
  8. Fraser K. B., Shirodaria P. V., Stanford C. F. Fluorescent staining and human IgM. Br Med J. 1971 Sep 18;3(5776):707–707. doi: 10.1136/bmj.3.5776.707. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Haire M., Hadden D. S. Rapid diagnosis of rubella by demonstrating rubella-specific IgM antibodies in ther serum by indirect immunofluorescence. J Med Microbiol. 1972 May;5(2):237–242. doi: 10.1099/00222615-5-2-237. [DOI] [PubMed] [Google Scholar]
  10. Halonen P. E., Ryan J. M., Stewart J. A. Rubella hemagglutinin prepared with alkaline extraction of virus grown in suspension culture of BHK-21 cells. Proc Soc Exp Biol Med. 1967 May;125(1):162–167. doi: 10.3181/00379727-125-32038. [DOI] [PubMed] [Google Scholar]
  11. Hornsleth A., Leerhoy J., Grauballe P., Spanggaard H. Rubellavirus-specific IgM- and IgA-antibodies. The indirect immunofluorescence (IF)-technique applied to sera with reduced IgG-concentration. Acta Pathol Microbiol Scand B Microbiol Immunol. 1974 Oct;82B(5):742–744. doi: 10.1111/j.1699-0463.1974.tb00244.x. [DOI] [PubMed] [Google Scholar]
  12. Iwakata S., Rhodes A. J., Labzoffsky N. A. The significance of specific IgM antibody in the diagnosis of rubella employing the immunofluorescence technique. Can Med Assoc J. 1972 Feb 19;106(4):327–330. [PMC free article] [PubMed] [Google Scholar]
  13. Langenhuysen M. M., The T. H., Nieweg H. O., Kapsenberg J. G. Demonstration of IgM cytomegalovirus-antibodies as an aid to early diagnosis in adults. Clin Exp Immunol. 1970 Mar;6(3):387–393. [PMC free article] [PubMed] [Google Scholar]
  14. Ogra P. L., Kerr-Grant D., Umana G., Dzierba J., Weintraub D. Antibody response in serum and nasopharynx after naturally acquired and vaccine-induced infection with rubella virus. N Engl J Med. 1971 Dec 9;285(24):1333–1339. doi: 10.1056/NEJM197112092852401. [DOI] [PubMed] [Google Scholar]
  15. Pead P. J. Estimation of rubella-specific IgM antibody in sera by single fraction collection on sephadex G-200. Med Lab Technol. 1974 Apr;31(2):159–162. [PubMed] [Google Scholar]
  16. Ross C. A., McDaid R. Specific IgM antibody in serum of patients with herpes zoster infections. Br Med J. 1972 Dec 2;4(5839):522–523. doi: 10.1136/bmj.4.5839.522. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Schmidt N. J., Magoffin R. L., Lennette E. H. Association of group B coxsackie viruses with cases of pericarditis, myocarditis, or pleurodynia by demonstration of immunoglobulin M antibody. Infect Immun. 1973 Sep;8(3):341–348. doi: 10.1128/iai.8.3.341-348.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Schmitz H., Haas R. Determination of different cytomegalovirus immunoglobulins (IgG, IgA, IgM) by immunofluorescence. Arch Gesamte Virusforsch. 1972;37(1):131–140. doi: 10.1007/BF01241158. [DOI] [PubMed] [Google Scholar]
  19. Schmitz H., Scherer M. IgM antibodies to Epstein-Barr virus in infectious mononucleosis. Arch Gesamte Virusforsch. 1972;37(4):332–339. doi: 10.1007/BF01241456. [DOI] [PubMed] [Google Scholar]
  20. Skaug K., Tjotta E. Diagnosis of recent Herpes simplex infections. A modified immunofluorescent test for the detection of specific Herpes simplex IgM antibodies after staphylococcal adsorption of IgG. Acta Pathol Microbiol Scand B Microbiol Immunol. 1974 Jun;82(3):323–328. [PubMed] [Google Scholar]
  21. Urquhart G. E. Serum IgM and IgA responses in influenza A infections. J Clin Pathol. 1974 Mar;27(3):198–201. doi: 10.1136/jcp.27.3.198. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Webb A. J. A 30-minute preparative method for isolation of IgG from human serum. Vox Sang. 1972;23(4):279–290. doi: 10.1111/j.1423-0410.1972.tb03462.x. [DOI] [PubMed] [Google Scholar]
  23. Weeke B. Rocket immunoelectrophoresis. Scand J Immunol Suppl. 1973;1:37–46. doi: 10.1111/j.1365-3083.1973.tb03777.x. [DOI] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES