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. 1975 Apr;11(4):823–828. doi: 10.1128/iai.11.4.823-828.1975

Preparation and chemical composition of the cell walls of Streptococcus mutans.

H R Cooper, F W Chorpenning, S Rosen
PMCID: PMC415141  PMID: 1120614

Abstract

Purified cell walls from Streptococcus mutans strain BHT were prepared without the use of proteolytic enzymes in order to retain all cell wall constituents for chemical analysis. Of four methods employed, the Ribi cell fractionator produced disrupted cell suspensions which could be most thoroughly purified on sucrose gradients. Results of chemical analyses on purified cell walls prepared in this 8.9% glycerol teichoic acid, 33.6% non-peptidoglycan polysaccharide, and 49.9% peptidoglycan.

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Selected References

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  1. BOAS N. F. Method for the determination of hexosamines in tissues. J Biol Chem. 1953 Oct;204(2):553–563. [PubMed] [Google Scholar]
  2. Bleiweis A. S., Craig R. A., Zinner D. D., Jablon J. M. Chemical composition of purified cell walls of cariogenic streptococci. Infect Immun. 1971 Jan;3(1):189–191. doi: 10.1128/iai.3.1.189-191.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bratthall D. Demonstration of five serological groups of streptococcal strains resembling Streptococcus mutans. Odontol Revy. 1970;21(2):143–152. [PubMed] [Google Scholar]
  4. Bratthall D. Immunodiffusion studies on the serological specificity of streptococci resembling Streptococcus mutans. Odontol Revy. 1969;20(3):231–243. [PubMed] [Google Scholar]
  5. Bratthall D. Immunofluorescent identification of Streptococcus mutans. Odontol Revy. 1972;23(2):181–196. [PubMed] [Google Scholar]
  6. Carlsson J. Presence of various types of non-haemolytic streptococci in dental plaque and in other sites of the oral cavity in man. Odontol Revy. 1967;18(1):55–74. [PubMed] [Google Scholar]
  7. Coykendall A. L. Genetic heterogeneity in Streptococcus mutans. J Bacteriol. 1971 Apr;106(1):192–196. doi: 10.1128/jb.106.1.192-196.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. DISCHE Z. Spectrophotometric method for the determination of free pentose and pentose in nucleotides. J Biol Chem. 1949 Nov;181(1):379–392. [PubMed] [Google Scholar]
  9. Drucker D. B., Shuttleworth C. A., Melville T. H. A quantitative analysis of the cell wall amino acids of cariogenic and non-cariogenic streptococci. Arch Oral Biol. 1968 Aug;13(8):937–940. doi: 10.1016/0003-9969(68)90009-5. [DOI] [PubMed] [Google Scholar]
  10. Edwardsson S. Characteristics of caries-inducing human streptococci resembling Streptococcus mutans. Arch Oral Biol. 1968 Jun;13(6):637–646. doi: 10.1016/0003-9969(68)90142-8. [DOI] [PubMed] [Google Scholar]
  11. FITZGERALD R. J., JORDAN H. V., STANLEY H. R. Experimental caries and gingival pathologic changes in the gnotobiotic rat. J Dent Res. 1960 Sep-Oct;39:923–935. doi: 10.1177/00220345600390052701. [DOI] [PubMed] [Google Scholar]
  12. FITZGERALD R. J., KEYES P. H. Demonstration of the etiologic role of streptococci in experimental caries in the hamster. J Am Dent Assoc. 1960 Jul;61:9–19. doi: 10.14219/jada.archive.1960.0138. [DOI] [PubMed] [Google Scholar]
  13. HAGEN J. H., HAGEN P. B. An enzymic method for the estimation of glycerol in blood and its use to determine the effect of noradrenaline on the concentration of glycerol in blood. Can J Biochem Physiol. 1962 Aug;40:1129–1139. [PubMed] [Google Scholar]
  14. JANCZURA E., PERKINS H. R., ROGERS H. J. Teichuronic acid: a mucopolysaccharide present in wall preparations from vegetative cells of Bacillus subtilis. Biochem J. 1961 Jul;80:82–93. doi: 10.1042/bj0800082. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Jablon J. M., Zinner D. D. Differentiation of cariogenic streptococci by fluorescent antibody. J Bacteriol. 1966 Dec;92(6):1590–1596. doi: 10.1128/jb.92.6.1590-1596.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Linzer R., Slade H. D. Purification and characterization of Streptococcus mutans group d cell wall polysaccharide antigen. Infect Immun. 1974 Aug;10(2):361–368. doi: 10.1128/iai.10.2.361-368.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Mukasa H., Slade H. D. Extraction, purification, and chemical and immunological properties of the Streptococcus mutans group "a" polysaccharide cell wall antigen. Infect Immun. 1973 Aug;8(2):190–198. doi: 10.1128/iai.8.2.190-198.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Mukasa H., Slade H. D. Structure and immunological specificity of the Streptococcus mutans group b cell wall antigen. Infect Immun. 1973 Apr;7(4):578–585. doi: 10.1128/iai.7.4.578-585.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. ORLAND F. J., BLAYNEY J. R., HARRISON R. W., REYNIERS J. A., TREXLER P. C., ERVIN R. F., GORDON H. A., WAGNER M. Experimental caries in germfree rats inoculated with enterococci. J Am Dent Assoc. 1955 Mar;50(3):259–272. doi: 10.14219/jada.archive.1955.0061. [DOI] [PubMed] [Google Scholar]
  20. SALTON M. R. Studies of the bacterial cell wall. IV. The composition of the cell walls of some Gram-positive and Gram-negative bacteria. Biochim Biophys Acta. 1953 Apr;10(4):512–523. doi: 10.1016/0006-3002(53)90296-0. [DOI] [PubMed] [Google Scholar]
  21. STIENECKER C. D., RHEINS M. S. A micro-modification of the anthrone test for serum samples of limited quantity. Am J Med Technol. 1959 Nov-Dec;25:377–380. [PubMed] [Google Scholar]
  22. Schleifer K. H., Kandler O. Peptidoglycan types of bacterial cell walls and their taxonomic implications. Bacteriol Rev. 1972 Dec;36(4):407–477. doi: 10.1128/br.36.4.407-477.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Simonson L. G., Shklair I. L. Gel electrophoresis of intracellular components of some cariogenic streptococci. J Dent Res. 1972 Mar-Apr;51(2):488–491. doi: 10.1177/00220345720510024001. [DOI] [PubMed] [Google Scholar]
  24. Slade H. D., Slamp W. C. Peptidoglycan composition and taxonomy of group D, E, and H streptococci, and Streptococcus mutans. J Bacteriol. 1972 Feb;109(2):691–695. doi: 10.1128/jb.109.2.691-695.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Soprey P., Slade H. D. Chemical structure and immunological specificity of the streptococcal group e cell wall polysaccharide antigen. Infect Immun. 1971 May;3(5):653–658. doi: 10.1128/iai.3.5.653-658.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Vaught R. M., Bleiweis A. S. Antigens of Streptococcus mutans. II. Characterization of an antigen resembling a glycerol teichoic acid in walls of strain BHT. Infect Immun. 1974 Jan;9(1):60–67. doi: 10.1128/iai.9.1.60-67.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. ZINNER D. D., JABLON J. M., ARAN A. P., SASLAW M. S. EXPERIMENTAL CARIES INDUCED IN ANIMALS BY STREPTOCOCCI OF HUMAN ORIGIN. Proc Soc Exp Biol Med. 1965 Mar;118:766–770. doi: 10.3181/00379727-118-29964. [DOI] [PubMed] [Google Scholar]

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