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. 1975 Jun;11(6):1216–1221. doi: 10.1128/iai.11.6.1216-1221.1975

Platelet Kinetics in Canine Ehrlichiosis: Evidence for Increased Platelet Destruction as the Cause of Thrombocytopenia

Ronald D Smith *, Miodrag Ristic †,‡, David L Huxsoll †,‡,1, Richard A Baylor †,‡
PMCID: PMC415202  PMID: 1140846

Abstract

A significant (P < 0.025) increase in the mean platelet diameter occurred in five Ehrlichia canis-infected dogs when platelet numbers decreased to 100,000/μl or less. Maximal incorporation of [75Se]selenomethionine into platelets of six uninfected dogs was 0.080 ± 0.019% (mean ± standard error) and occurred 5 to 6 days after dosage, whereas maximal incorporation was 0.036 ± 0.004% within 2 to 3 days after dosage in seven chronically infected dogs that had thrombocytopenia. Analysis of the [75Se]selenomethionine curves yielded a platelet lifespan of 9 days in uninfected dogs versus 4 days in chronically infected dogs. Thus, megakaryocyte maturation and/or platelet release occurred at an accelerated rate in infected dogs, whereas increased destruction of newly produced labeled platelets diminished their number of peripheral blood. [51Cr]sodium chromate-labeled platelet survival was exponential, with a half-life of approximately 1 day in two dogs at 2 to 4 days postinfection and three chronically infected dogs. Platelet survival time was 8 days and rectilinear in four uninfected dogs. Platelet recovery was 39.43 ± 2.86% in infected dogs as compared with 68.2 ± 10.72% in uninfected dogs. Whole-body scans of one dog prior to and 7 days after infection showed that labeled platelets were destroyed primarily in the spleen. It is concluded that the thrombocytopenia in E. canis-infected dogs is the result of increased platelet destruction which begins within a few days after infection.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Abrahamsen A. F. A modification of the technique for 51Cr-labelling of blood platelets giving increased circulating platelet radioactivity. Scand J Haematol. 1968;5(1):53–63. doi: 10.1111/j.1600-0609.1968.tb01718.x. [DOI] [PubMed] [Google Scholar]
  2. Amyx H. L., Huxsoll D. L., Zeiler D. C., Hildebrandt P. K. Therapeutic and prophylactic value of tetracycline in dogs infected with the agent of tropical canine pancytopenia. J Am Vet Med Assoc. 1971 Dec 1;159(11):1428–1432. [PubMed] [Google Scholar]
  3. Branehög I., Kutti J., Weinfeld A. Platelet survival and platelet production in idiopathic thrombocytopenic purpura (ITP). Br J Haematol. 1974 May;27(1):127–143. doi: 10.1111/j.1365-2141.1974.tb06780.x. [DOI] [PubMed] [Google Scholar]
  4. Brodsky I., Siegel N. H., Kahn S. B., Ross E. M., Petkov G. Simultaneous fibrinogen and platelet survival with (75Se)selenomethionine in man. Studies in diseases with normal coagulation and in hepatocellular disease with abnormal coagulation. Br J Haematol. 1970 Mar;18(3):341–355. doi: 10.1111/j.1365-2141.1970.tb01448.x. [DOI] [PubMed] [Google Scholar]
  5. Buhles W. C., Jr, Huxsoll D. L., Ristic M. Tropical canine pancytopenia: Clinical, hematologic, and serologic response of dogs to Ehrlichia canis infection, tetracycline therapy, and challenge inoculation. J Infect Dis. 1974 Oct;130(4):357–367. doi: 10.1093/infdis/130.4.357. [DOI] [PubMed] [Google Scholar]
  6. Chang Y. H. The effect of diisopropylfluorophosphonate (DFP) on platelet survival in the rabbit. Blood. 1967 Jun;29(6):891–895. [PubMed] [Google Scholar]
  7. Cooney D. P., Smith B. A., Fawley D. E. The use of 32di-isopropylfluorophosphate (32DFP) as a platelet label: evidence for reutilization of this isotope in man. Blood. 1968 Jun;31(6):791–805. [PubMed] [Google Scholar]
  8. Fein R. The new national health spending policy. N Engl J Med. 1974 Jan 17;290(3):137–140. doi: 10.1056/NEJM197401172900305. [DOI] [PubMed] [Google Scholar]
  9. Garg S. K., Amorosi E. L., Karpatkin S. Use of the megathrombocyte as an index of megakaryocyte number. N Engl J Med. 1971 Jan 7;284(1):11–17. doi: 10.1056/NEJM197101072840103. [DOI] [PubMed] [Google Scholar]
  10. Harker L. A. Regulation of thrombopoiesis. Am J Physiol. 1970 May;218(5):1376–1380. doi: 10.1152/ajplegacy.1970.218.5.1376. [DOI] [PubMed] [Google Scholar]
  11. Hildebrandt P. K., Huxsoll D. L., Walker J. S., Nims R. M., Taylor R., Andrews M. Pathology of canine ehrlichiosis (tropical canine pancytopenia). Am J Vet Res. 1973 Oct;34(10):1309–1320. [PubMed] [Google Scholar]
  12. Huxsoll D. L., Amyx H. L., Hemelt I. E., Hildebrandt P. K., Nims R. M., Gochenour W. S., Jr Laboratory studies of tropical canine pancytopenia. Exp Parasitol. 1972 Feb;31(1):53–59. doi: 10.1016/0014-4894(72)90046-x. [DOI] [PubMed] [Google Scholar]
  13. Huxsoll D. L., Hildebrandt P. K., Nims R. M., Walker J. S. Tropical canine pancytopenia. J Am Vet Med Assoc. 1970 Dec 1;157(11):1627–1632. [PubMed] [Google Scholar]
  14. Karpatkin S. Biochemical and clinical aspects of megathrombocytes. Ann N Y Acad Sci. 1972 Oct 27;201:262–279. doi: 10.1111/j.1749-6632.1972.tb16304.x. [DOI] [PubMed] [Google Scholar]
  15. Karpatkin S., Garg S. K. The megathrombocyte as an index of platelet production. Br J Haematol. 1974 Mar;26(3):307–311. doi: 10.1111/j.1365-2141.1974.tb00475.x. [DOI] [PubMed] [Google Scholar]
  16. Kraytman M. Platelet size in thrombocytopenias and thrombocytosis of various origin. Blood. 1973 Apr;41(4):587–598. [PubMed] [Google Scholar]
  17. Lombardi M. H. Radioisotopic blood volume and cardiac output in dogs. Am J Vet Res. 1972 Sep;33(9):1825–1834. [PubMed] [Google Scholar]
  18. MIZUNO N. S., PERMAN V., BATES F. W., SAUTTER J. H., SCHULTZE M. O. Life span of thrombocytes and erythrocytes in normal and thrombocytopenic calves. Blood. 1959 Jun;14(6):708–719. [PubMed] [Google Scholar]
  19. Minter F. M., Ingram M. Platelet volume: density relationships in normal and acutely bled dogs. Br J Haematol. 1971 Jan;20(1):55–68. doi: 10.1111/j.1365-2141.1971.tb00786.x. [DOI] [PubMed] [Google Scholar]
  20. Najean Y., Ardaillou N. The use of 75Se-methionin for the in vivo study of platelet kinetics. Scand J Haematol. 1969;6(6):395–401. doi: 10.1111/j.1600-0609.1969.tb01833.x. [DOI] [PubMed] [Google Scholar]
  21. Pirkle H., Carstens P. Pulmonary platelet aggregates associated with sudden death in man. Science. 1974 Sep 20;185(4156):1062–1064. doi: 10.1126/science.185.4156.1062. [DOI] [PubMed] [Google Scholar]
  22. Ries C. A., Price D. C. (51Cr)platelet kinetics in thrombocytopenia. Correlation between splenic sequestration of platelets and response to splenectomy. Ann Intern Med. 1974 Jun;80(6):702–707. doi: 10.7326/0003-4819-80-6-702. [DOI] [PubMed] [Google Scholar]
  23. Rolovic Z., Baldini M., Dameshek W. Megakaryocytopoiesis in experimentally induced immune thrombocytopenia. Blood. 1970 Feb;35(2):173–188. [PubMed] [Google Scholar]
  24. Sahud M. A. Platelet size and number in alcoholic thrombocytopenia. N Engl J Med. 1972 Feb 17;286(7):355–356. doi: 10.1056/NEJM197202172860706. [DOI] [PubMed] [Google Scholar]
  25. Seamer J., Snape T. Ehrlichia canis and tropical canine pancytopaenia. Res Vet Sci. 1972 Jul;13(4):307–314. [PubMed] [Google Scholar]
  26. Simpson C. F. Relationship of Ehrlichia canis-infected mononuclear cells to blood vessels of lungs. Infect Immun. 1974 Sep;10(3):590–596. doi: 10.1128/iai.10.3.590-596.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Smith R. D., Anderson J. J., Ristic M., Huxsoll D. L. The use of Cerenkov radiation in 32 P labeled platelet survival studies. Int J Appl Radiat Isot. 1972 Nov;23(11):513–517. doi: 10.1016/0020-708x(72)90133-0. [DOI] [PubMed] [Google Scholar]
  28. Smith R. D., Hooks J. E., Huxsoll D. L., Ristic M. Canine ehrlichiosis (tropical canine pancytopenia): survival of phosphorus-32-labeled blood platelets in normal and infected dogs. Am J Vet Res. 1974 Feb;35(2):269–273. [PubMed] [Google Scholar]
  29. Vainer H. The platelet populations. Adv Exp Med Biol. 1972;34:191–217. doi: 10.1007/978-1-4684-3231-2_11. [DOI] [PubMed] [Google Scholar]

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