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. 1975 Jun;11(6):1244–1251. doi: 10.1128/iai.11.6.1244-1251.1975

Adsorption of Typhus Rickettsiae to Ghosts of Sheep Erythrocytes

Herbert H Winkler 1, Louise E Ramm 1
PMCID: PMC415206  PMID: 806524

Abstract

Rickettsia prowazeki will adsorb to ghosts derived from sheep erythrocytes by hypotonic lysis. Adsorption to ghosts, as to intact erythrocytes, is dependent on the metabolism of the rickettsiae. KCN and 0 C inhibit adsorption. Fluoride, while inhibiting hemolysis, has no effect on adsorption to ghosts or intact erythrocytes. The adsorption of rickettsiae to ghosts does not lead to lysis, in that fluorescent albumin can be retained by the ghost after adsorption. Both inside-out and right-side-out vesicles formed from the ghost are equally capable of adsorption, indicating that the receptor is stable during the process of vesiculation and is not localized to one aspect of the cell membrane. Adsorption to ghosts is slower and displays less affinity than to the intact erythrocyte. The ghost system will be useful in characterizing the membrane receptor for rickettsia and in establishing assays for adsorption to other cells.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BOVARNICK M. R., MILLER J. C., SNYDER J. C. The influence of certain salts, amino acids, sugars, and proteins on the stability of rickettsiae. J Bacteriol. 1950 Apr;59(4):509–522. doi: 10.1128/jb.59.4.509-522.1950. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. COHN Z. A., BOZEMAN F. M., CAMPBELL J. M., HUMPHRIES J. W., SAWYER T. K. Study on growth of Rickettsia. V. Penetration of Rickettsia tsutsugamushi into mammalian cells in vitro. J Exp Med. 1959 Mar 1;109(3):271–292. doi: 10.1084/jem.109.3.271. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. DODGE J. T., MITCHELL C., HANAHAN D. J. The preparation and chemical characteristics of hemoglobin-free ghosts of human erythrocytes. Arch Biochem Biophys. 1963 Jan;100:119–130. doi: 10.1016/0003-9861(63)90042-0. [DOI] [PubMed] [Google Scholar]
  4. GIMENEZ D. F. STAINING RICKETTSIAE IN YOLK-SAC CULTURES. Stain Technol. 1964 May;39:135–140. doi: 10.3109/10520296409061219. [DOI] [PubMed] [Google Scholar]
  5. Kant J. A., Steck T. L. Cation-impermeable inside-out and right-side-out vesicles from human erythrocyte membranes. Nat New Biol. 1972 Nov 1;240(96):26–28. doi: 10.1038/newbio240026a0. [DOI] [PubMed] [Google Scholar]
  6. Ramm L. E., Winkler H. H. Rickettsial hemolysis: adsorption of rickettsiae to erythrocytes. Infect Immun. 1973 Jan;7(1):93–99. doi: 10.1128/iai.7.1.93-99.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Ramm L. E., Winkler H. H. Rickettsial hemolysis: effect of metabolic inhibitors upon hemolysis and adsorption. Infect Immun. 1973 Apr;7(4):550–555. doi: 10.1128/iai.7.4.550-555.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. SNYDER J. C., BOVARNICK M. R., MILLER J. C., CHANG R. S. M. Observations on the hemolytic properties of typhus rickettsiae. J Bacteriol. 1954 Jun;67(6):724–730. doi: 10.1128/jb.67.6.724-730.1954. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. WISSEMAN C. L., Jr, JACKSON E. B., HAHN F. E., LEY A. C., SMADEL J. E. Metabolic studies of rickettsiae. I. The effects of antimicrobial substances and enzyme inhibitors on the oxidation of glutamate by purified rickettsiae. J Immunol. 1951 Aug;67(2):123–136. [PubMed] [Google Scholar]
  10. Winkler H. H. Inhibitory and restorative effects of adenine nucleotides on rickettsial adsorption and hemolysis. Infect Immun. 1974 Jan;9(1):119–126. doi: 10.1128/iai.9.1.119-126.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]

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