Abstract
Three high temperature-sensitive (ts) mutants of foot-and-mouth disease virus were characterized by their relative abilities to grow at 33 or 38.5 C, to kill infant mice, to infect guinea pigs, and to produce foot-and-mouth disease in steers. Mutants ts-24 and ts-42 did not grow at 38.5 C; both may have produced considerable quantities of noninfectious virus particles at 33 C. A third mutant, ts-22, appeared “leaky” because it multiplied to a limited extent during prolonged incubation at the nonpermissive temperature. Mutant ts-42 was pathogenic for infant mice, whereas ts-22 and ts-24 were essentially apathogenic. Mice were protected against the lethal effects of the wild-type (wt) virus if injected 1 week earlier with ts-22, apparently as a result of specific antibody development. One-half of the guinea pigs injected with the mutant viruses showed lesion development, but only at the site of injection. Antibody development was also much less than in those animals injected with the wt virus. The onset of FMD in steers was delayed and the severity of the disease was diminished after intradermalingual injection of the mutant viruses.
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Selected References
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- BACHRACH H. L., CALLIS J. J., HESS W. R., PATTY R. E. A plaque assay for foot-and-mouth disease virus and kinetics of virus reproduction. Virology. 1957 Oct;4(2):224–236. doi: 10.1016/0042-6822(57)90060-0. [DOI] [PubMed] [Google Scholar]
- Campbell C. H. Influence of litter size, age, variation, and selective breeding of mice on susceptibility to foot-and-mouth disease virus. Am J Vet Res. 1968 Mar;29(3):685–691. [PubMed] [Google Scholar]
- Lake J., Mackenzie J. S. Improved technique for the isolation of temperature-sensitive mutants of foot-and-mouth disease virus. J Virol. 1973 Sep;12(3):665–668. doi: 10.1128/jvi.12.3.665-668.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Manor D., Goldblum N. Isolation and partial characterization of temperature-sensitive mutants of the SAT-1 strain of foot-and-mouth disease virus. Isr J Med Sci. 1973 Feb;9(2):145–149. [PubMed] [Google Scholar]
- McVicar J. W., Sutmoller P., Andersen A. A. Foot-and-mouth disease virus: plaque reduction neutralization tests. Arch Gesamte Virusforsch. 1974;44(2):168–172. doi: 10.1007/BF01250230. [DOI] [PubMed] [Google Scholar]
- Mills J., Van Kirk J., Hill D. A., Chanock R. M. Evaluation of influenza virus mutants for possible use in a live virus vaccine. Bull World Health Organ. 1969;41(3):599–606. [PMC free article] [PubMed] [Google Scholar]
- Mirchamsy H., Rapp F. A new overlay for plaquing animal viruses. Proc Soc Exp Biol Med. 1968 Oct;129(1):13–17. doi: 10.3181/00379727-129-33237. [DOI] [PubMed] [Google Scholar]
- PLATT H. The localization of lesions in experimental foot-and-mouth disease. Br J Exp Pathol. 1960 Apr;41:150–159. [PMC free article] [PubMed] [Google Scholar]
- PRUNET P. MODIFICATIONS DU COMPORTEMENT DES VIRUS APHTEUX LORS DES PASSAGES SUCCESSIFS EN CULTURE DE TISSU 'A DIVERSES TEMP'ERATURES. RECHERCHE DE MUTANTS "FROIDS". Ann Inst Pasteur (Paris) 1964 Jan;106:18–28. [PubMed] [Google Scholar]
- Richmond J. Y., Campbell C. H. Enhancement of primary antigen-specific resistance in infant mice with divinyl ether-maleic anhydride. Infect Immun. 1974 Nov;10(5):1029–1033. doi: 10.1128/iai.10.5.1029-1033.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Simpson R. W., Hirst G. K. Temperature-sensitive mutants of influenza A virus: isolation of mutants and preliminary observations on genetic recombination and complementation. Virology. 1968 May;35(1):41–49. doi: 10.1016/0042-6822(68)90303-6. [DOI] [PubMed] [Google Scholar]