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. 1975 Jun;11(6):1320–1324. doi: 10.1128/iai.11.6.1320-1324.1975

Simplified Assay for Concanavalin A-Dependent Bacterial Agglutination by Using Cell Surface Mutants

H B Maruyama 1, M Arisawa 1, M Ono-Onitsuka 1
PMCID: PMC415218  PMID: 1095486

Abstract

Two cell surface mutants of Escherichia coli isolated through supersensitivity to sodium dodecyl sulfate were found to be agglutinated by concanavalin A in the intact rod form. By using these strains, the assay method for the concanavalin A-dependent agglutination was greatly simplified since neither spheroplast formation nor protease treatment is needed. This rod cell agglutination was characterized and applied for a semiquantitative determination of the amount of bound concanavalin A.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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