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. 1975 Jul;12(1):55–61. doi: 10.1128/iai.12.1.55-61.1975

Demonstration by immunoelectro-osmophoresis of precipitating antibodies to a purified rubella virus antigen.

P C Grauballe, B F Vestergaard, A Hornsleth, J Leerhoy, T Johnsson
PMCID: PMC415244  PMID: 1140852

Abstract

The nonionic detergent Triton X-100 was used to extract antigens of rubella virus from infected tissue culture cells. Three virus-specific antigens were demonstrated by crossed immunoelectrophoresis by using a pool of human gamma globulin as antiserum. The most dominant of these antigens were purified by ion-exchange chromatography on diethylaminoethyl-cellulose. This antigen was of glucoprotein nature and had slow electrophoretic motility and low binding capacity to diethylaminoethyl-cellulose. Thus, it seems likely that the antigen is identical with the precipitating antigen of rubella virus designated b-antigen or tro-osmophoresis with precipiting antibody in sera obtained from patients recovering from acute postnatal rubella. The precipitin reaction that could be correlated to the hemaglutination-inhibition titers of the same sera appeared 12 days after onset of the disease and remained positive for several years.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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