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. 1975 Jul;12(1):137–142. doi: 10.1128/iai.12.1.137-142.1975

Biochemical and immunological properties of ribonucleic acid-rich extracts from Francisella tularensis.

I I Andron LA, H T Eigelsbach
PMCID: PMC415257  PMID: 237834

Abstract

Ribonucleic acid (RNA)-rich extracts derived from the attenuated strain of Francisella tularensis (strain LVS) protected Swiss mice against lethal challenge with F. tularensis strain 425 but not against strain SCHU S4. No killed preparation, including an RNA-rich extract from SCHU S4 itself, offered protection against strain SCHU S4 in contrast to the high level of protection offered against this strain by vaccination with live strain LVS. The protective activity observed against strain 425 was sensitive to ribonuclease but not to Pronase. Protective activity is not a general property of bacterial RNA, since RNA-rich extracts from Staphylococcus aureus offered no protection against tularemia, although disc gel electrophoresis showed similar kinds and amounts of RNA in preparations form F. tularensis and S. aureus. Furthermore, inability to localize activity to a specific region in sucrose gradients suggests a structural rather than an informational role for the RNA in such extracts. RNA-rich extracts from F. tularensis but not from S. aureus were efficient inducers of F. tularensis opsonins in mouse serum, suggesting one mechanism by which such extracts confer protection.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ALEXANDER M. M., WRIGHT G. G., BALDWIN A. C. Observations on the agglutination of polysaccharide-treated erythrocytes by tularemia antisera. J Exp Med. 1950 Jun 1;91(6):561–566. doi: 10.1084/jem.91.6.561. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Araujo F. G., Remington J. S. Protection against Toxoplasma gondii in mice immunized with Toxoplasma cell fractions, RNA and synthetic polyribonucleotides. Immunology. 1974 Oct;27(4):711–721. [PMC free article] [PubMed] [Google Scholar]
  3. Archer S. J., Wust C. J. Preliminary characterization of "immunogenic" ribonucleic acid derived from rat peritoneal exudate cells. Infect Immun. 1973 Aug;8(2):215–221. doi: 10.1128/iai.8.2.215-221.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Bell C., Dray S. Conversion of non-immune rabbit spleen cells by ribonucleic acid of lymphoid cells from an immunized rabbit to produce IgM and IgG antibody of foreign heavy-chain allotype. J Immunol. 1971 Jul;107(1):83–95. [PubMed] [Google Scholar]
  5. Bourque D. P., Naylor A. W. A simple electrophoretic procedure for separation of RNA on mixed agarose-acrylamide gel columns. J Chromatogr. 1971 Mar 24;56(1):79–86. doi: 10.1016/s0021-9673(00)97779-3. [DOI] [PubMed] [Google Scholar]
  6. DeChatelet L. R., Mullikin D., Shirley P. S., McCall C. E. Phagocytosis of live versus heat-killed bacteria by human polymorphonuclear leukocytes. Infect Immun. 1974 Jul;10(1):25–29. doi: 10.1128/iai.10.1.25-29.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Duke L. J., Miller C., Harshman S. Induction of antibody synthesis by purified immunogenic RNA in vitro. Nat New Biol. 1972 Feb 9;235(58):180–182. doi: 10.1038/newbio235180a0. [DOI] [PubMed] [Google Scholar]
  8. EIGELSBACH H. T., DOWNS C. M. Prophylactic effectiveness of live and killed tularemia vaccines. I. Production of vaccine and evaluation in the white mouse and guinea pig. J Immunol. 1961 Oct;87:415–425. [PubMed] [Google Scholar]
  9. Fishman M., Adler F. L. The formation of immunogenic RNA-antigen complexes in a cell-free system. Cell Immunol. 1973 Aug;8(2):221–234. doi: 10.1016/0008-8749(73)90112-3. [DOI] [PubMed] [Google Scholar]
  10. Guentzel M. N., Berry L. J. Protection of suckling mice from experimental cholera by maternal immunization: comparison of the efficacy of whole-cell, ribosomal-derived, and enterotoxin immunogens. Infect Immun. 1974 Jul;10(1):167–172. doi: 10.1128/iai.10.1.167-172.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Jensen R., Gregory B., Naylor J., Actor P. Isolation of protective somatic antigen from Vibrio cholerae (Ogawa) ribosomal preparations. Infect Immun. 1972 Aug;6(2):156–161. doi: 10.1128/iai.6.2.156-161.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  13. Margolis J. M., Bigley N. J. Cytophilic macroglobulin reactive with bacterial protein in mice immunized with ribonucleic acid-protein fractions of virulent Salmonella typhimurium. Infect Immun. 1972 Sep;6(3):390–397. doi: 10.1128/iai.6.3.390-397.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Meiss H. K., Fishman M. Formation of antibody in rabbit spleen cell cultures stimulated with immunogenic RNA. J Immunol. 1972 May;108(5):1172–1178. [PubMed] [Google Scholar]
  15. Metzger J. F., Johnson A. D., Collins W. S., 2nd, McGann V. Staphylococcus aureus enterotoxin B release (excretion) under controlled conditions of fermentation. Appl Microbiol. 1973 May;25(5):770–773. doi: 10.1128/am.25.5.770-773.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Neiburger R. G., Youmans G. P., Youmans A. S. Relationship between tuberculin hypersensitivity and cellular immunity to infection in mice vaccinated with viable attenuated Mycobacterial cells or with Mycobacterial ribonucleic acid preparations. Infect Immun. 1973 Jul;8(1):42–47. doi: 10.1128/iai.8.1.42-47.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Proctor R. A., White J. D., Ayala E., Canonico P. G. Phagocytosis of Francisella tularensis by Rhesus monkey peripheral leukocytes. Infect Immun. 1975 Jan;11(1):146–151. doi: 10.1128/iai.11.1.146-151.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. SASLAW S., EIGELSBACH H. T., PRIOR J. A., WILSON H. E., CARHART S. Tularemia vaccine study. II. Respiratory challenge. Arch Intern Med. 1961 May;107:702–714. doi: 10.1001/archinte.1961.03620050068007. [DOI] [PubMed] [Google Scholar]
  19. Smith R. A., Bigley N. J. Detection of delayed hypersensitivity in mice injected with ribonucleic acid-protein fractions of Salmonella typhimurium. Infect Immun. 1972 Sep;6(3):384–389. doi: 10.1128/iai.6.3.384-389.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Smith R. A., Bigley N. J. Ribonucleic acid-protein fractions of virulent Salmonella typhimurium as protective immunogens. Infect Immun. 1972 Sep;6(3):377–383. doi: 10.1128/iai.6.3.377-383.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Thomas D. W., Weiss E. Response of mice to injection of ribosomal fraction from group B Neisseria meningitidis. Infect Immun. 1972 Sep;6(3):355–363. doi: 10.1128/iai.6.3.355-363.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Thompson H. C., Snyder I. S. Protection against pneumococcal infection by a ribosomal preparation. Infect Immun. 1971 Jan;3(1):16–23. doi: 10.1128/iai.3.1.16-23.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Veltman L. L., Kern D. H., Pilch Y. H. Immune cytolysis of human tumor cells mediated by xenogeneic "immune" RNA. Cell Immunol. 1974 Sep;13(3):367–377. doi: 10.1016/0008-8749(74)90256-1. [DOI] [PubMed] [Google Scholar]
  24. Venneman M. R., Bigley N. J., Berry L. J. Immunogenicity of Ribonucleic Acid Preparations Obtained from Salmonella typhimurium. Infect Immun. 1970 Jun;1(6):574–582. doi: 10.1128/iai.1.6.574-582.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Vyas G. N., Ibrahim A. B., Rao K. R., Likhite V. In vitro transfer of cell-mediated immunity to hepatitis B antigen with RNA. Nature. 1974 Feb 8;247(5440):377–378. doi: 10.1038/247377a0. [DOI] [PubMed] [Google Scholar]
  26. Winston S. H., Berry L. J. Antibacterial immunity induced by ribosomal vaccines. J Reticuloendothel Soc. 1970 Jul;8(1):13–24. [PubMed] [Google Scholar]
  27. Winston S., Berry L. J. Immunity induced by ribosomal extracts from Staphylococcus aureus. J Reticuloendothel Soc. 1970 Jul;8(1):66–73. [PubMed] [Google Scholar]
  28. Youmans A. S., Youmans G. P. The effect of metabolic inhibitors and hydroxylamine on the immune response in mice to mycobacterial ribonucleic acid vaccines. J Immunol. 1974 Jan;112(1):271–284. [PubMed] [Google Scholar]

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