Skip to main content
NCBI home page
Infection and Immunity logoLink to Infection and Immunity
. 1975 Jul;12(1):148–155. doi: 10.1128/iai.12.1.148-155.1975

Effect of human leukocyte interferon on vaccinia-and herpes virus-infected cell cultures and monkey corneas.

D Neumann-Haefelin, R Sundmacher, B Sauter, H E Karges, K F Manthey
PMCID: PMC415259  PMID: 166924

Abstract

Pretreatment of human fibroblast cultures with human leukocyte interferon (HIF, 1,000 IU/ml) resulted in a 24-h delay of virus replication after infection with vaccinia virus and herpes simplex virus type 1 and type 2. Additional HIF treatment 24 h after infection effectively lowered the maximum yield of viral infectivity. Equal results were obtained in simian cells with 3,000 IU of HIF per ml. The spread of two cell-bound herpesviruses, varicella zoster virus and Medical Lake macaque herpesvirus, was inhibited by 2,000 IU of HIF per ml in human fibroblasts and Vero cells, respectively. Varicella zoster virus infectivity was notably reduced by HIF, whereas the latter system showed a low sensitivity. To study the effect of HIF in the infected cornea, keratitis was induced experimentally in both eyes of 12 rhesus monkeys and 12 African green monkeys by inoculation with vaccinia virus and herpes simplex virus, respectively. In each monkey one eye served as a control for the full cycle of disease. In the other eye HIF treatment was initiated prophylactically 15 h before or simultaneously with the challenge virus infection or 6 to 20 h postinfectionally or therapeutically after onset of the disease, and the treatment was continued for 2 to 7 days. Prophylactic and simultaneous administration equally resulted in inhibition of both vaccinia and herpes keratitis. Postinfectional and therapeutic administration of interferon moderated the course of keratitis slightly and shortened the period of virus shedding.

Full text

PDF
148

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Armstrong R. W., Merigan T. C. Varicella zoster virus: interferon production and comparative interferon sensitivity in human cell cultures. J Gen Virol. 1971 Jul;12(1):53–54. doi: 10.1099/0022-1317-12-1-53. [DOI] [PubMed] [Google Scholar]
  2. Billiau A., Joniau M., De Somer P. Mass production of human interferon in diploid cells stimulated by poly-I:C. J Gen Virol. 1973 Apr;19(1):1–8. doi: 10.1099/0022-1317-19-1-1. [DOI] [PubMed] [Google Scholar]
  3. Blakely G. A., Lourie B., Morton W. G., Evans H. H., Kaufmann A. F. A varicella-like disease in macaque monkeys. J Infect Dis. 1973 Jun;127(6):617–625. doi: 10.1093/infdis/127.6.617. [DOI] [PubMed] [Google Scholar]
  4. Frayssinet C., Gresser I., Tovey M., Lindahl P. Letter: Inhibitory effect of potent interferon preparations on the regeneration of mouse liver after partial hepatectomy. Nature. 1973 Sep 21;245(5421):146–147. doi: 10.1038/245146b0. [DOI] [PubMed] [Google Scholar]
  5. Gallagher J. G., Khoobyarian N. Sensitivity of adenovirus types 1, 3, 4, 5, 8, 11, and 18 to human interferon. Proc Soc Exp Biol Med. 1971 Mar;136(3):920–924. doi: 10.3181/00379727-136-35395. [DOI] [PubMed] [Google Scholar]
  6. Glasgow L. A., Hanshaw J. B., Merigan T. C., Petralli J. K. Interferon and cytomegalovirus in vivo and in vitro. Proc Soc Exp Biol Med. 1967 Jul;125(3):843–849. doi: 10.3181/00379727-125-32220. [DOI] [PubMed] [Google Scholar]
  7. Goore M. Y., Dickson J. H., Lipkin S., DiCuollo C. J. The production of human leukocyte interferon in a serum-free medium. Proc Soc Exp Biol Med. 1973 Jan;142(1):46–49. doi: 10.3181/00379727-142-36954. [DOI] [PubMed] [Google Scholar]
  8. HO M., ENDERS J. F. Further studies on an inhibitor of viral activity appearing in infected cell cultures and its role in chronic viral infections. Virology. 1959 Nov;9:446–477. doi: 10.1016/0042-6822(59)90135-7. [DOI] [PubMed] [Google Scholar]
  9. Hallum J. V., Younger J. S. Quantitative aspects of inhibition of virus replication by interferon in chick embryo cell cultures. J Bacteriol. 1966 Oct;92(4):1047–1050. doi: 10.1128/jb.92.4.1047-1050.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Ho M., Tan Y. H., Armstrong J. A. Accentuation of production of human interferon by metabolic inhibitors. Proc Soc Exp Biol Med. 1972 Jan;139(1):259–262. doi: 10.3181/00379727-139-36122. [DOI] [PubMed] [Google Scholar]
  11. ISAACS A., BURKE D. C., FADEEVA L. Effect of interferon on the growth of viruses on the chick chorion. Br J Exp Pathol. 1958 Oct;39(5):447–451. [PMC free article] [PubMed] [Google Scholar]
  12. JONES B. R., GALBRAITH J. E., AL-HUSSAINI M. K. Vaccinial keratitis treated with interferon. Lancet. 1962 Apr 28;1(7235):875–879. doi: 10.1016/s0140-6736(62)91908-6. [DOI] [PubMed] [Google Scholar]
  13. Jordan G. W., Fried R. P., Merigan T. C. Administration of human leukocyte interferon in herpes zoster. I. Safety, circulating, antiviral activity, and host responses to infection. J Infect Dis. 1974 Jul;130(1):56–62. doi: 10.1093/infdis/130.1.56. [DOI] [PubMed] [Google Scholar]
  14. Kaufman H. E., Ellison E. D., Centifanto Y. M. Difference in interferon response and protection from ocular virus infection in rabbits and monkeys. Am J Ophthalmol. 1972 Jul;74(1):89–92. doi: 10.1016/0002-9394(72)91131-2. [DOI] [PubMed] [Google Scholar]
  15. Kaufman H. E., Sugar J., Varnell E. D. Effect of exogenous interferon on herpetic keratitis in rabbits and monkeys. Invest Ophthalmol. 1973 May;12(5):378–380. [PubMed] [Google Scholar]
  16. McNeill T. A., Gresser I. Inhibition of haemopoietic colony growth by interferon preparations from different sources. Nat New Biol. 1973 Aug 8;244(136):173–174. doi: 10.1038/newbio244173a0. [DOI] [PubMed] [Google Scholar]
  17. Merigan T. C., Reed S. E., Hall T. S., Tyrrell D. A. Inhibition of respiratory virus infection by locally applied interferon. Lancet. 1973 Mar 17;1(7803):563–567. doi: 10.1016/s0140-6736(73)90714-9. [DOI] [PubMed] [Google Scholar]
  18. Myers M. W., Friedman R. M. Potentiation of human interferon production by superinduction. J Natl Cancer Inst. 1971 Oct;47(4):757–764. [PubMed] [Google Scholar]
  19. Strander H., Cantell K., Carlström G., Jakobsson P. A. Clinical and laboratory investigations on man: systemic administration of potent interferon to man. J Natl Cancer Inst. 1973 Sep;51(3):733–742. doi: 10.1093/jnci/51.3.733. [DOI] [PubMed] [Google Scholar]
  20. Strander H., Cantell K. Production of interferon by human leukocytes in vitro. Ann Med Exp Biol Fenn. 1966;44(2):265–273. [PubMed] [Google Scholar]
  21. TOMMILA V., PENTTINEN K. Effect of ultraviolet irradiated influenza Lee viruses on experimental herpes simplex virus infection in rabbits' eyes. Acta Ophthalmol (Copenh) 1962;40:520–525. doi: 10.1111/j.1755-3768.1962.tb07824.x. [DOI] [PubMed] [Google Scholar]
  22. Youngner J. S., Thacore H. R., Kelly M. E. Sensitivity of ribonucleic acid and deoxyribonucleic acid viruses to different species of interferon in cell cultures. J Virol. 1972 Aug;10(2):171–178. doi: 10.1128/jvi.10.2.171-178.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES