Skip to main content
Infection and Immunity logoLink to Infection and Immunity
. 1975 Jul;12(1):156–161. doi: 10.1128/iai.12.1.156-161.1975

Chemical composition and immunological specificity of cell wall polysaccharide group antigens of streptococcal groups P and U.

G E Wessman
PMCID: PMC415260  PMID: 49302

Abstract

The group antigens of streptococcal groups P and U were extracted with formamide and purified on diethylaminoethyl-Sephadex A-25 and Sephadex G-200. The antigens were shown to be polysaccharides located in the cell walls of the organisms. In a precipitin test, the P and U group antigens did not cross-react with homologous sera of each other, nor with specific antiserum of the group antigen of group E streptococci. The polysaccharide comprising the group P antigen contained rhamnose, glucose, galactose, glucosamine, and galactosamine; the group U antigen was similar in composition but lacked galactosamine and contained more galactose.

Full text

PDF
156

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BLEIWEIS A. S., KARAKAWA W. W., KRAUSE R. M. IMPROVED TECHNIQUE FOR THE PREPARATION OF STREPTOCOCCAL CELL WALLS. J Bacteriol. 1964 Oct;88:1198–1200. doi: 10.1128/jb.88.4.1198-1200.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bleiweis A. S., Coleman S. E. Improved separation of components of streptococcal cell walls by thin-layer chromatography. Anal Biochem. 1969 May;29(2):343–347. doi: 10.1016/0003-2697(69)90319-4. [DOI] [PubMed] [Google Scholar]
  3. Ellis R. P., Armstrong C. H. Humoral antibody responses of swine infected experimentally with group E streptococcus. I. Whole cell agglutinin response. Can J Comp Med. 1972 Jul;36(3):195–201. [PMC free article] [PubMed] [Google Scholar]
  4. Ellis R. P., Armstrong C. H. Humoral antibody responses of swine infected experimentally with group E streptococcus. II. Antistreptokinase and antistreptodornase responses. Can J Comp Med. 1972 Jul;36(3):210–220. [PMC free article] [PubMed] [Google Scholar]
  5. KUNTER E. GAMMA-HAEMOLYTISCHE STREPTOKOKKEN DER GRUPPE P IN MILCH. Zentralbl Bakteriol Orig. 1963 Jul;189:348–350. [PubMed] [Google Scholar]
  6. Karakawa W. W., Wagner J. E., Pazur J. H. Immunochemistry of the cell-wall carbohydrate of group L hemolytic streptococci. J Immunol. 1971 Aug;107(2):554–562. [PubMed] [Google Scholar]
  7. Krantz G. E., Dunne H. W. An attempt to classify Streptococcic isolates from domestic animals. Am J Vet Res. 1965 Jul;26(113):951–959. [PubMed] [Google Scholar]
  8. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  9. LUEDERITZ O., SIMMONS D. A., WESTPHAL O., STROMINGER J. L. A SPECIFIC MICRODETERMINATION OF GLUCOSAMINE AND THE ANALYSIS OF OTHER HEXOSAMINES IN THE PRESENCE OF GLUCOSAMINE. Anal Biochem. 1964 Nov;9:263–271. doi: 10.1016/0003-2697(64)90184-8. [DOI] [PubMed] [Google Scholar]
  10. Matsuno T., Slade H. D. Composition and properties of a group A streptococcal teichoic acid. J Bacteriol. 1970 Jun;102(3):747–752. doi: 10.1128/jb.102.3.747-752.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Mukasa H., Slade H. D. Chemical composition and immunological specificity of the streptococcal group O cell wall polysaccharide antigen. Infect Immun. 1972 May;5(5):707–714. doi: 10.1128/iai.5.5.707-714.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Payne J. B., Armstrong C. H. Somatic antigens of Streptococcus group E. I. Comparison of extraction techniques. Appl Microbiol. 1970 May;19(5):818–822. doi: 10.1128/am.19.5.818-822.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. SLADE H. D., SLAMP W. C. Studies on Streptococcus pyogenes. V. Biochemical and microscopic aspects of cell lysis and digestion by enzymes from Streptomyces albus. J Bacteriol. 1960 Jan;79:103–112. doi: 10.1128/jb.79.1.103-112.1960. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Shuman R. D., Nord N., Brown R. W., Wessman G. E. Biochemical and serological characteristics of Lancefield groups E, P, and U Streptococci and Streptococcus uberis. Cornell Vet. 1972 Oct;62(4):540–568. [PubMed] [Google Scholar]
  15. Shuman R. D., Wood R. L., Nord N. Swine abscesses caused by Lancefield's group E streptococci. V. Specificity of the precipitin test for their detection with relation to streptococcal antigens of groups P and U and Corynebacterium pyogenes. Cornell Vet. 1969 Jan;59(1):102–120. [PubMed] [Google Scholar]
  16. Slade H. D. Extraction of Cell-Wall Polysaccharide Antigen from Streptococci. J Bacteriol. 1965 Sep;90(3):667–672. doi: 10.1128/jb.90.3.667-672.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Soprey P., Slade H. D. Chemical structure and immunological specificity of the streptococcal group e cell wall polysaccharide antigen. Infect Immun. 1971 May;3(5):653–658. doi: 10.1128/iai.3.5.653-658.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Wessman G. E., Shuman R. D., Nord N. Swine abscesses caused by Lancefield's group E streptococci. VI. Experimental application of hemagglutination tests for their detection. Cornell Vet. 1970 Apr;60(2):286–296. [PubMed] [Google Scholar]
  19. Wessman G. E., Shuman R. D., Wood R. L., Nord N. Swine abscesses caused by Lancefield's group E streptococci. IX. Comparison of the precipitin, hemagglutination and agglutination tests for their detection. Cornell Vet. 1971 Jul;61(3):400–414. [PubMed] [Google Scholar]
  20. de Moor C. E., Thal E. Beta haemolytic streptococci of the Lancefield groups E, P and U: streptococcus infrequens. Antonie Van Leeuwenhoek. 1968;34(4):377–387. doi: 10.1007/BF02046460. [DOI] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES