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. 1975 Jul;12(1):180–186. doi: 10.1128/iai.12.1.180-186.1975

Interaction of hepatitis B surface antigen (Australia antigen) with membrane vesicles of Pseudomonas aeruginosa.

L K Weng, M E Bayer, W T London
PMCID: PMC415264  PMID: 49303

Abstract

A lysogenic strain of Pseudomonas aeruginosa was cultured from the dialysis fluid of a patient on chronic hemodialysis treatment whose blood contained hepatitis B surface antigen (HB8Ag). When this bacterium was incubated for 4 to 7 days with serum containing HB8Ag or with purified HB8Ag, a loss of the HB8Ag-specific immunological reactivity was observed. Bacteriophages can be induced from the isolated P. aeruginosa with mitomycin C; the phages, after purification on CsCl gradients, also lyse P. aeruginosa strain 25102 (ATCC). Subsequent to gradient centrifugation of the lysate, a fraction was found with a density around 1.40 g/ml that inactivated HB8Ag after a 4-h incubation at 37 C as determined by counterelectrophoresis and hemagglutination inhibition. The activity was not found in appreciable amounts in other gradient fractions. The electron microscope shows that the active fraction contains envelope vesicles of 45 to 60 nm in diameter. In spite of their loss in HB8Ag activity, the HB8Ag particles (22nm) appeared morphologically intact. These findings suggest that an enzyme(s) is present in the vesicle fraction which inactivates antigenic determinants on HB8Ag particles. Thus, the presence of these bacteria in environments such as feces, dialysis tanks, and contaminated drinking water may prevent the detection of HB8Ag.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bartell P. F., Orr T. E. Origin of polysaccharide depolymerase associated with bacteriophage infection. J Virol. 1969 Mar;3(3):290–296. doi: 10.1128/jvi.3.3.290-296.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bradley D. E. Ultrastructure of bacteriophage and bacteriocins. Bacteriol Rev. 1967 Dec;31(4):230–314. doi: 10.1128/br.31.4.230-314.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Cheng K. J., Ingram J. M., Costerton J. W. Alkaline phosphatase localization and spheroplast formation of Pseudomonas aeruginosa. Can J Microbiol. 1970 Dec;16(12):1319–1324. doi: 10.1139/m70-218. [DOI] [PubMed] [Google Scholar]
  4. Finkelstein A. E., Strawich E. S., Sonnino S. Characterization and partial purification of a lipase from Pseudomonas aeruginosa. Biochim Biophys Acta. 1970 Jun 10;206(3):380–391. doi: 10.1016/0005-2744(70)90154-3. [DOI] [PubMed] [Google Scholar]
  5. Fishman L. S., Armstrong D. Pseudomonas aeruginosa bacteremia in patients with neoplastic disease. Cancer. 1972 Sep;30(3):764–773. doi: 10.1002/1097-0142(197209)30:3<764::aid-cncr2820300326>3.0.co;2-g. [DOI] [PubMed] [Google Scholar]
  6. Lache M., Hearn W. R., Zyskind J. W., Tipper D. J., Strominger J. L. Specificity of a bacteriolytic enzyme from Pseudomonas aeruginosa. J Bacteriol. 1969 Oct;100(1):254–259. doi: 10.1128/jb.100.1.254-259.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Lewis R. J. Infections in heroin addicts. JAMA. 1973 Feb 26;223(9):1036–1037. [PubMed] [Google Scholar]
  8. Lewis R., Gorbach S., Altner P. Spinal Pseudomonas chondro-osteomyelitis in heroin users. N Engl J Med. 1972 Jun 15;286(24):1303–1303. doi: 10.1056/NEJM197206152862407. [DOI] [PubMed] [Google Scholar]
  9. Mazzur S., Corbett J., Blumberg B. S. Loss of immunologic reactivities of Australia antigen after incubation with bacteria. Proc Soc Exp Biol Med. 1973 Jan;142(1):327–332. doi: 10.3181/00379727-142-37016. [DOI] [PubMed] [Google Scholar]
  10. Millman I., Hutanen H., Merino F., Bayer M. E., Blumberg B. S. Australia antigen: physical and chemical properties. Res Commun Chem Pathol Pharmacol. 1971 Jul-Sep;2(4):667–686. [PubMed] [Google Scholar]
  11. Millman I., Loeb L. A., Bayer M. E., Blumberg B. S. Australia antigen (a hepatitis-associated antigen): purification and physical properties. J Exp Med. 1970 Jun 1;131(6):1190–1199. doi: 10.1084/jem.131.6.1190. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Moody M. R., Young V. M., Kenton D. M., Vermeulen G. D. Pseudomonas aeruginosa in a center for cancer research. I. Distribution of intraspecies types from human and environmental sources. J Infect Dis. 1972 Feb;125(2):95–101. doi: 10.1093/infdis/125.2.95. [DOI] [PubMed] [Google Scholar]
  13. Neurath A. R., Prince A. M., Lippin A. Hepatitis B antigen: antigenic sites related to human serum proteins revealed by affinity chromatography. Proc Natl Acad Sci U S A. 1974 Jul;71(7):2663–2667. doi: 10.1073/pnas.71.7.2663. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Schimpff S. C., Greene W. H., Young V. M., Wiernik P. H. Pseudomonas septicemia: incidence, epidemiology, prevention and therapy in patients with advanced cancer. Eur J Cancer. 1973 Jun;9(6):449–455. doi: 10.1016/0014-2964(73)90110-2. [DOI] [PubMed] [Google Scholar]
  15. Thacker W. L., Eagon R. G. Characterization of a membrane-associated ATPase from Pseudomonas aeruginosa. Proc Soc Exp Biol Med. 1969 Dec;132(3):1127–1132. doi: 10.3181/00379727-132-34380. [DOI] [PubMed] [Google Scholar]

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