Skip to main content
NCBI home page
Infection and Immunity logoLink to Infection and Immunity
. 1975 Aug;12(2):364–377. doi: 10.1128/iai.12.2.364-377.1975

Evidence for O antigens as the antigenic determinants in "ribosomal" vaccines prepared from Salmonella.

T K Eisenstein
PMCID: PMC415293  PMID: 51007

Abstract

The nature of the protective substance in ribosomal ribonucleic acid and protein extracts of Salmonella has been investigated. The results of experiments in which vaccines were prepared from isogenic strains and strains with defects in lipopolysaccharide synthesis show that O antigens contaminate both ribonucleic acid and protein ribosomal extracts, and are responsible for at least part of their strain-specific protective activity. In addition, it was observed that a ribosomal ribonucleic acid preparation from S. adelaide contains a heat-stable immunogen which is not an O antigen or that gives cross-protection across species lines. The contribution of ribosomes to the immunity induced by "ribosomal vaccines" is discussed.

Full text

PDF
364

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Fogel S., Sypherd P. S. Extraction and isolation of individual ribosomal proteins from Escherichia coli. J Bacteriol. 1968 Aug;96(2):358–364. doi: 10.1128/jb.96.2.358-364.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Gorzynski E. A., Ambrus J. L., Neter E. Effect of common enterobacterial antiserum on experimental Salmonella typhimurium infection of mice. Proc Soc Exp Biol Med. 1971 Sep;137(4):1209–1212. doi: 10.3181/00379727-137-35757. [DOI] [PubMed] [Google Scholar]
  3. Jensen R., Gregory B., Naylor J., Actor P. Isolation of protective somatic antigen from Vibrio cholerae (Ogawa) ribosomal preparations. Infect Immun. 1972 Aug;6(2):156–161. doi: 10.1128/iai.6.2.156-161.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Johnson W. Ribosomal vaccines. I. Immunogenicity of ribosomal fractions isolated from Salmonella typhimurium and Yersinia pestis. Infect Immun. 1972 Jun;5(6):947–952. doi: 10.1128/iai.5.6.947-952.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Johnson W. Ribosomal vaccines. II. Specificity of the immune response to ribosomal ribonucleic acid and protein isolated from Salmonella typhimurium. Infect Immun. 1973 Sep;8(3):395–400. doi: 10.1128/iai.8.3.395-400.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. KUNIN C. M. SEPARATION, CHARACTERIZATION, AND BIOLOGICAL SIGNIFICANCE OF A COMMON ANTIGEN IN ENTEROBACTERIACEAE. J Exp Med. 1963 Oct 1;118:565–586. doi: 10.1084/jem.118.4.565. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Kasai N., Nowotny A. Endotoxic glycolipid from a heptoseless mutant of Salmonella minnesota. J Bacteriol. 1967 Dec;94(6):1824–1836. doi: 10.1128/jb.94.6.1824-1836.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Krishnapillai V., MacPhee D. G., Stocker B. A. Properties of a Salmonella typhimurium mutant with an incomplete deficiency of uridinediphosphogalactose-4-epimerase. J Bacteriol. 1971 Jul;107(1):155–161. doi: 10.1128/jb.107.1.155-161.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Krishnapillai V. Uridinediphosphogalactose-4-epimerase deficiency in Salmonella typhimurium and its correction by plasmoid-borne galactose genes of Escherichia coli K-12: effects on mouse virulence, phagocytosis, and serum sensitivity. Infect Immun. 1971 Sep;4(3):177–188. doi: 10.1128/iai.4.3.177-188.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Kurland C. G. The requirements for specific sRNA binding by ribosomes. J Mol Biol. 1966 Jun;18(1):90–108. doi: 10.1016/s0022-2836(66)80079-7. [DOI] [PubMed] [Google Scholar]
  11. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  12. Lüderitz O., Galanos C., Lehmann V., Nurminen M., Rietschel E. T., Rosenfelder G., Simon M., Westphal O. Lipid A: chemical structure and biological activity. J Infect Dis. 1973 Jul;128(Suppl):17–29. doi: 10.1093/infdis/128.supplement_1.s17. [DOI] [PubMed] [Google Scholar]
  13. MacPhee D. G., Stocker B. A. Suppression of amber and ochre mutants in Salmonella typhimurium by a mutant F'-1-gal factor carrying an ochre suppressor gene. J Bacteriol. 1969 Oct;100(1):240–246. doi: 10.1128/jb.100.1.240-246.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. McCabe W. R. Immunization with R mutants of S. Minnesota. I. Protection against challenge with heterologous gram-negative bacilli. J Immunol. 1972 Mar;108(3):601–610. [PubMed] [Google Scholar]
  15. McCabe W. R., Kreger B. E., Johns M. Type-specific and cross-reactive antibodies in gram-negative bacteremia. N Engl J Med. 1972 Aug 10;287(6):261–267. doi: 10.1056/NEJM197208102870601. [DOI] [PubMed] [Google Scholar]
  16. Morgenroth A., Duguid J. P. Demonstration of different mutational sites controlling rhamnose fermentation in FIRN and non-FIRN rha-strains of Salmonella typhimurium: an essay in bacterial archaeology. Genet Res. 1968 Apr;11(2):151–169. doi: 10.1017/s0016672300011320. [DOI] [PubMed] [Google Scholar]
  17. Mullan N. A., Newsome P. M., Cunnington P. G., Palmer G. H., Wilson M. E. Protection against gram-negative infections with antiserum to lipid A from Salmonella minnesota R595. Infect Immun. 1974 Dec;10(6):1195–1201. doi: 10.1128/iai.10.6.1195-1201.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. NETER E., BERTRAM L. F., ZAK D. A., MURDOCK M. R., ARBESMAN C. E. Studies on hemagglutination and hemolysis by escherichia coli antisera. J Exp Med. 1952 Jul;96(1):1–15. doi: 10.1084/jem.96.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. O'NEILL G. J., TODD J. P. Extraction of nucleic acid-free lipopolysaccharides from gram-negative bacteria. Nature. 1961 Apr 22;190:344–345. doi: 10.1038/190344b0. [DOI] [PubMed] [Google Scholar]
  20. Pace N. R. Structure and synthesis of the ribosomal ribonucleic acid of prokaryotes. Bacteriol Rev. 1973 Dec;37(4):562–603. doi: 10.1128/br.37.4.562-603.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Rudbach J. A., Anacker R. L., Haskins W. T., Milner K. C., Ribi E. Physical structure of a native protoplasmic polysaccharide from Escherichia coli. J Immunol. 1967 Jan;98(1):1–7. [PubMed] [Google Scholar]
  22. Sanderson K. E. Linkage map of Salmonella typhimurium, edition IV. Bacteriol Rev. 1972 Dec;36(4):558–586. doi: 10.1128/br.36.4.558-586.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Smith R. A., Bigley N. J. Ribonucleic acid-protein fractions of virulent Salmonella typhimurium as protective immunogens. Infect Immun. 1972 Sep;6(3):377–383. doi: 10.1128/iai.6.3.377-383.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Tripodi D., Nowotny A. Relation of structure to function in bacterial O-antigens. V. Nature of active sites in endotoxic lipopolysaccharides of Serratia marcescens. Ann N Y Acad Sci. 1966 Jun 30;133(2):604–621. doi: 10.1111/j.1749-6632.1966.tb52392.x. [DOI] [PubMed] [Google Scholar]
  25. Venneman M. R., Bigley N. J. Isolation and partial characterization of an immunogenic moiety obtained from Salmonella typhimurium. J Bacteriol. 1969 Oct;100(1):140–148. doi: 10.1128/jb.100.1.140-148.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Venneman M. R. Purification of immunogenically active ribonucleic acid preparations of Salmonella typhimurium: molecular-sieve and anion-exchange chromatography. Infect Immun. 1972 Mar;5(3):269–282. doi: 10.1128/iai.5.3.269-282.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Wilkinson R. G., Gemski P., Jr, Stocker B. A. Non-smooth mutants of Salmonella typhimurium: differentiation by phage sensitivity and genetic mapping. J Gen Microbiol. 1972 May;70(3):527–554. doi: 10.1099/00221287-70-3-527. [DOI] [PubMed] [Google Scholar]
  28. YOUMANS A. S., YOUMANS G. P. IMMUNOGENIC ACTIVITY OF A RIBOSOMAL FRACTION OBTAINED FROM MYCOBACTERIUM TUBERCULOSIS. J Bacteriol. 1965 May;89:1291–1298. doi: 10.1128/jb.89.5.1291-1298.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Youmans A. S., Youmans G. P. Preparation of highly immunogenic ribosomal fractions of Mycobacterium tuberculosis by use of sodium dodecyl sulfate. J Bacteriol. 1966 Jun;91(6):2139–2145. doi: 10.1128/jb.91.6.2139-2145.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Ziegler E. J., Douglas H., Sherman J. E., Davis C. E., Braude A. I. Treatment of E. coli and klebsiella bacteremia in agranulocytic animals with antiserum to a UDP-gal epimerase-deficient mutant. J Immunol. 1973 Aug;111(2):433–438. [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES