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. 1975 Aug;12(2):404–410. doi: 10.1128/iai.12.2.404-410.1975

Plasmid-linked ampicillin resistance in haempohilus influenza type b.

L P Elwell, J De Graaff, D Seibert, S Falkow
PMCID: PMC415297  PMID: 1080132

Abstract

Four ampicillin-resistant, beta-lactamase-producing strains of Haempohilus influenzae type b were examined for the presence of plasmid deoxyribonucleic acid (DNA). Three resistant strains contained a 30 x 10-6-dalton (30Mdal) plasmid and one resitant strain contained a 3-Mdal plasmid. The ampicillin-sensitive Haemophilus strains examined did not contain plasmid DNA. Transformation of a sensitive H. influenzae strain to ampicillin resistance with isolated plasmid DNA preparations revealed that the structural gene for beta-lactamase resided on both plasmid species. DNA-DNA hybridization studies showed that the 30-Mdal Haemophilus plasmid contained the ampicillin translocation DNA segment (TnA) found on some R-factors of enteric origin of the H. influenzae plasmids.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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